##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526524_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67513 Sequences flagged as poor quality 0 Sequence length 56 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.53037192836935 39.0 38.0 40.0 35.0 40.0 2 38.22041680861464 39.0 38.0 40.0 35.0 40.0 3 38.2613126360849 39.0 38.0 40.0 35.0 40.0 4 38.31722779316576 39.0 38.0 40.0 35.0 40.0 5 38.232029386932886 39.0 38.0 40.0 35.0 40.0 6 38.25968332024944 39.0 38.0 40.0 35.0 40.0 7 38.20286463347799 39.0 38.0 40.0 35.0 40.0 8 38.42508850147379 39.0 38.0 40.0 36.0 40.0 9 38.30743708619081 39.0 38.0 40.0 35.0 40.0 10 37.51927776872602 39.0 38.0 40.0 33.0 40.0 11 37.524802630604476 39.0 38.0 40.0 33.0 40.0 12 37.51321967621051 39.0 38.0 40.0 33.0 40.0 13 37.502658747204244 39.0 38.0 40.0 33.0 40.0 14 37.50314754195488 39.0 38.0 40.0 33.0 40.0 15 37.49206819427369 39.0 38.0 40.0 33.0 40.0 16 37.4315465169671 39.0 38.0 40.0 33.0 40.0 17 37.432598166279085 39.0 38.0 40.0 33.0 40.0 18 37.29172159435961 39.0 38.0 40.0 33.0 40.0 19 37.1210137306889 39.0 37.0 40.0 33.0 40.0 20 37.24801149408262 39.0 37.0 40.0 33.0 40.0 21 37.11258572423089 39.0 37.0 40.0 33.0 40.0 22 37.00833913468517 39.0 37.0 40.0 33.0 40.0 23 36.74784115651801 39.0 36.0 40.0 31.0 40.0 24 36.70387925288463 39.0 36.0 40.0 31.0 40.0 25 36.67717328514508 39.0 36.0 40.0 31.0 40.0 26 36.33312102854265 39.0 36.0 40.0 31.0 40.0 27 36.275295128345654 39.0 36.0 40.0 31.0 40.0 28 36.0906047724142 39.0 36.0 40.0 30.0 40.0 29 36.04453957015686 39.0 36.0 40.0 30.0 40.0 30 35.73091108379127 38.0 35.0 40.0 29.0 40.0 31 35.42852487669042 38.0 35.0 40.0 29.0 40.0 32 35.062565728082 38.0 35.0 40.0 27.0 40.0 33 35.15524417519589 38.0 35.0 40.0 27.0 40.0 34 35.27022943729356 38.0 35.0 40.0 28.0 40.0 35 35.03369721386992 38.0 35.0 40.0 27.0 40.0 36 34.68293513841779 39.0 35.0 40.0 25.0 40.0 37 34.174647845600106 38.0 35.0 40.0 21.0 40.0 38 34.3351058314695 38.0 34.0 40.0 23.0 40.0 39 34.30299349754862 38.0 34.0 40.0 23.0 40.0 40 33.851554515426656 38.0 34.0 40.0 19.0 40.0 41 33.65694014486099 38.0 33.0 40.0 17.0 40.0 42 33.58308770162783 38.0 33.0 40.0 17.0 40.0 43 33.45671204064402 38.0 33.0 40.0 16.0 40.0 44 32.98021121858012 38.0 33.0 40.0 9.0 40.0 45 33.0550560632767 38.0 33.0 40.0 7.0 40.0 46 32.651874453808894 38.0 33.0 40.0 4.0 40.0 47 32.425829099580824 38.0 32.0 40.0 4.0 40.0 48 32.0910787552027 38.0 32.0 39.0 4.0 40.0 49 31.97249418630486 37.0 32.0 39.0 4.0 40.0 50 31.693866366477568 37.0 31.0 39.0 4.0 40.0 51 31.066090975071468 37.0 31.0 39.0 4.0 40.0 52 30.827944247774504 36.0 31.0 39.0 4.0 40.0 53 30.44360345414957 36.0 30.0 39.0 4.0 40.0 54 30.115622176469717 36.0 30.0 39.0 4.0 40.0 55 29.454179195117977 36.0 28.0 39.0 4.0 40.0 56 17.32167138180795 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.23402492076770898 1 2 0.3891038181770554 1 3 -0.06328308851427522 1 4 -0.45051218062936726 1 5 -0.004162113379223342 1 6 -0.23713015530012171 1 7 -0.41945336339289696 1 8 -0.7806941125563895 1 9 -0.788058364577644 1 10 0.4935094997221512 1 11 0.03647007336019925 1 12 -0.5906538394621563 1 13 -0.9027990412708249 1 14 -0.7942901749236029 1 15 -0.565274304010579 1 16 -0.5106226887951735 1 17 -0.22713677234160912 1 18 -0.23175137940876311 1 19 -0.6876121476442378 1 20 -0.14641617219579928 1 21 -0.20813295035299006 1 22 -0.6421140541051358 1 23 -0.4898069819320483 1 24 0.13541547678141796 1 25 0.41702665114566173 1 26 -0.24905533266629476 1 27 -0.0818543759503001 1 28 -0.62945386824412 1 29 0.13232529214354827 1 30 -0.16937740396046053 1 31 0.5176045804311826 1 32 0.7571052411593158 1 33 0.07032116664254318 1 34 0.48978526849721504 1 35 0.87081489309994 1 36 1.2450245887280076 1 37 1.5212415728438984 1 38 1.4111227995036373 1 39 1.0338071934977862 1 40 1.7532677015252105 1 41 1.8000603731872786 1 42 1.4773265772151376 1 43 2.0530935252307714 1 44 2.4753720564603725 1 45 1.461105958438381 1 46 0.5683505234350292 1 47 0.18158132504476754 1 48 0.09062342966437598 1 49 -0.49253442430305583 1 50 0.8996033699549493 1 51 1.7031286186001715 1 52 1.431013279425347 1 53 1.7631765819980565 1 54 2.0820785740826615 1 55 1.6003038867413473 1 56 1.1537355718336642 2 1 -0.6673082790885445 2 2 0.1634666367677653 2 3 -0.498065697209924 2 4 -0.36030728307814286 2 5 0.008831389869150996 2 6 -0.42628557758897756 2 7 -0.03664476769074554 2 8 -0.25445723099716844 2 9 -0.14113182784601008 2 10 -0.6356759075742033 2 11 -0.7678777527267542 2 12 -1.2843070128754448 2 13 -1.4745131841993597 2 14 -1.1995875262479387 2 15 -1.7454342240505625 2 16 -0.9716421790500505 2 17 -0.8073466673940786 2 18 -0.9121611745112119 2 19 -1.1821148962699226 2 20 -1.0089848878379826 2 21 -0.6871434451056189 2 22 -0.5783959131756049 2 23 -1.1797120294083143 2 24 -1.3271032638482652 2 25 -0.9535380664955184 2 26 -1.158600560052605 2 27 -1.5061422320222633 2 28 -2.15294212411618 2 29 -1.4603783560323578 2 30 -2.1069086383432634 2 31 -0.88619352051834 2 32 -1.2161581271565254 2 33 -1.1483195130176256 2 34 -1.5739328724323158 2 35 -1.4240376806132033 2 36 -0.32993793001262617 2 37 -0.10769395938998372 2 38 -0.27578374722299515 2 39 -0.16409385597747672 2 40 -0.3989062115182662 2 41 0.2585811128174669 2 42 -1.0389153018453285 2 43 -0.5373612473829184 2 44 -0.20828611445416811 2 45 -0.045140918123337315 2 46 -0.9146579723170944 2 47 -1.5702927379237472 2 48 -1.8506559306554635 2 49 -1.6227193318492823 2 50 -1.7997969298951269 2 51 -3.268135749215922 2 52 -3.446048189840024 2 53 -3.1463686453882502 2 54 -2.8619494119243356 2 55 -2.4854032596854374 2 56 -1.9517117045281562 3 1 0.3435612861288462 3 2 0.8962032096577914 3 3 -0.1662242649848622 3 4 0.19553244411708448 3 5 0.6388399150183375 3 6 0.2167238000751297 3 7 0.7291267583109544 3 8 0.6138292140156238 3 9 0.40362520540207214 3 10 0.8449293780182998 3 11 0.5658818380660833 3 12 0.8510297305175598 3 13 0.8345234739421556 3 14 0.5403954234536812 3 15 0.47174395156751103 3 16 0.24191280816222616 3 17 0.4381776292811068 3 18 0.15262995933363044 3 19 1.1851059050940975 3 20 -0.03434720667856084 3 21 0.12807394619873236 3 22 -0.38146496688404596 3 23 0.07966563470080956 3 24 0.5897765315481536 3 25 -0.34057983972654426 3 26 0.8290379736217375 3 27 0.1377196605608617 3 28 1.4344406550824544 3 29 1.0481467931577484 3 30 1.0927929814350819 3 31 0.45928815041089877 3 32 0.9863141661086061 3 33 1.1306659942287496 3 34 0.7732538283349442 3 35 1.4651353799153526 3 36 0.9833613027239494 3 37 1.4370630738580985 3 38 1.8563560652237214 3 39 1.3512514125647286 3 40 1.5915029956428555 3 41 1.9917439431669948 3 42 2.011809335835828 3 43 1.8720590424721522 3 44 1.9490028881033723 3 45 2.233418331663529 3 46 2.5800138094390874 3 47 2.191216213482903 3 48 2.9161812389950086 3 49 2.498337786650289 3 50 3.0953437350665105 3 51 2.153432878235055 3 52 3.4695528331778824 3 53 3.6500934176978497 3 54 3.1403950041029454 3 55 3.142900235625728 3 56 0.8586037259918768 4 1 -0.21199426942671096 4 2 0.5040463469126877 4 3 0.3256058003746105 4 4 0.23148015653538323 4 5 0.2793627908353358 4 6 0.36296563014048644 4 7 0.6825581308599737 4 8 0.3131756192443831 4 9 0.3709454668399843 4 10 0.9331646721359448 4 11 -0.2829743710842436 4 12 -0.3344277858223066 4 13 -0.07233927115588301 4 14 -0.31009477262475116 4 15 -0.08299461052398982 4 16 0.4788409127373896 4 17 0.038667825359539165 4 18 -0.054069387071599806 4 19 0.13853727764311685 4 20 -0.1454583177896751 4 21 0.11418505730984663 4 22 0.9469664056649734 4 23 0.23816236672695368 4 24 -0.3138182396936742 4 25 0.5458580687701868 4 26 0.6680902612034387 4 27 -0.3998620387855496 4 28 0.09947333482101328 4 29 -0.10953294540433944 4 30 0.5772701056180836 4 31 1.1904973007376967 4 32 1.5435037085922687 4 33 1.7139993275620853 4 34 1.5395936976159916 4 35 1.7510830923336513 4 36 2.4212697994559775 4 37 2.4738277797404535 4 38 2.127597895289078 4 39 1.695205660930739 4 40 2.600489923747432 4 41 1.389619760160464 4 42 1.9423648913913851 4 43 1.6776145980277093 4 44 2.4571728227438996 4 45 1.7211634297027487 4 46 2.1976608682626164 4 47 2.0776541219796343 4 48 2.5738609775570964 4 49 1.8259521657352593 4 50 2.55286007493579 4 51 2.995753139672967 4 52 3.626415578275921 4 53 3.98587773142334 4 54 2.9647414093317046 4 55 2.42884794804403 4 56 2.182950131220636 5 1 0.2602279527955105 5 2 0.7616221044884455 5 3 0.7094441842129982 5 4 0.19107611613134878 5 5 0.865221376693917 5 6 0.2581676503425072 5 7 0.5575581308599737 5 8 0.42933723540599544 5 9 0.4517535476480603 5 10 0.2740737630450383 5 11 0.20313674002686355 5 12 -0.3811449575394761 5 13 0.31402436520775723 5 14 0.46642037889039756 5 15 -0.028701681231062537 5 16 -0.6903510064545273 5 17 -0.6242109625192498 5 18 -0.1815946395968524 5 19 -0.47131120720536757 5 20 0.048986126654774864 5 21 0.3629224310472168 5 22 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6 7 1.017875591177436 6 8 0.7496835557523198 6 9 0.8947549906495098 6 10 0.7784027673740397 6 11 0.20313674002686355 6 12 1.2270801506856301 6 13 1.0506766018599905 6 14 1.22760364007366 6 15 0.3277196751902949 6 16 0.7764599603564406 6 17 0.1954138571055708 6 18 0.49156553356332466 6 19 1.0849658490716934 6 20 0.8426369203055657 6 21 0.560613628738416 6 22 0.42712513582370804 6 23 0.07149570006028227 6 24 0.6107849349094963 6 25 0.8573660052781236 6 26 0.09467756279073569 6 27 0.3620427231192167 6 28 0.8256638110114878 6 29 0.8011813403099453 6 30 0.6784605818085652 6 31 1.1131163483567477 6 32 1.0950910101795728 6 33 0.753681867244623 6 34 -0.11913646111416654 6 35 -0.871932780682215 6 36 -0.856507978321801 6 37 -0.5321246012119261 6 38 -1.1025608348696494 6 39 -0.731381640656565 6 40 -7.00552443042568E-4 6 41 0.08604833158903347 6 42 0.8451426691691637 6 43 0.33634475675786746 6 44 0.429395044966121 6 45 0.6477507312900457 6 46 -0.4412280206262693 6 47 0.3931303124558241 6 48 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0.123418238506801 7 33 0.4652813788441321 7 34 0.0481407061630037 7 35 0.7583480495986095 7 36 0.011868090054264258 7 37 0.9411354720481455 7 38 -0.33821407052288777 7 39 0.9616586523837327 7 40 1.2686523169098294 7 41 1.7112009567416564 7 42 0.7887324127589039 7 43 1.1143667347798427 7 44 1.6191386347097065 7 45 1.382060865600181 7 46 2.016891637493387 7 47 2.016329335654845 7 48 2.050570379266503 7 49 2.3761658409489357 7 50 3.2383301604058765 7 51 2.7421206610404845 7 52 2.954620706481048 7 53 2.324980295525908 7 54 2.4247841443744385 7 55 2.5438052130012956 7 56 2.2237620970326013 8 1 0.4144568085169027 8 2 0.919469232484829 8 3 0.7874631801424385 8 4 0.5291584318256 8 5 1.0786994409182498 8 6 0.43116629349040636 8 7 0.6968616134470409 8 8 0.015497343954166354 8 9 0.5765839411351763 8 10 0.8473437766135561 8 11 0.35736559574825577 8 12 0.1396601080417028 8 13 0.5856209278490923 8 14 0.06366144628071879 8 15 0.02376326675627638 8 16 0.6707977949927795 8 17 0.04923996466302327 8 18 0.6820417240395145 8 19 0.4289601632152582 8 20 -0.17986959473826403 8 21 -0.40550814335350793 8 22 -0.40253608189721035 8 23 0.3766366619176651 8 24 -0.7943323358794174 8 25 0.007093557990749844 8 26 -1.138916372295732 8 27 -1.8857658530475732 8 28 -1.6770606618622352 8 29 -2.323670590512137 8 30 -1.3881113205842368 8 31 -2.990887442214209 8 32 -2.4768113826183438 8 33 -2.970080274427964 8 34 -2.7796434499793676 8 35 -2.6191490801373263 8 36 -1.8283156068458482 8 37 -1.0019184889162602 8 38 -1.9926342771818994 8 39 -1.7448855496828628 8 40 -1.3586725969822524 8 41 -0.5815659778163251 8 42 -0.9807279775970059 8 43 -0.24361259931889379 8 44 -0.36662485553139135 8 45 -1.6140355752723785 8 46 -1.7015928630806663 8 47 -1.3678268067102515 8 48 -1.0851357056933182 8 49 -1.6157974196710434 8 50 -1.3559293114655375 8 51 -1.5765934606587102 8 52 -1.6472162625201001 8 53 -0.7386247561388473 8 54 -0.9765935823764238 8 55 -1.2697424333821736 8 56 -0.784504263472563 9 1 0.3364184289859864 9 2 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-0.9270010564554454 120 54 -0.7031878835975931 120 55 0.10814087733695743 120 56 0.18926326253376757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 115.0 5 13.0 6 28.0 7 27.0 8 98.0 9 120.0 10 86.0 11 99.0 12 149.0 13 135.0 14 194.0 15 205.0 16 209.0 17 275.0 18 334.0 19 341.0 20 398.0 21 442.0 22 554.0 23 495.0 24 652.0 25 766.0 26 766.0 27 867.0 28 997.0 29 1175.0 30 1257.0 31 1737.0 32 2169.0 33 2536.0 34 3385.0 35 4397.0 36 5999.0 37 9401.0 38 13102.0 39 13990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 98.90621294759308 0.7217808868864122 0.26825942613232157 0.10374673938819065 8 98.52324737457971 1.1849569712499815 0.17478115325937227 0.11701450091093568 9 93.8500733192126 3.8318546057796277 0.9864766785656096 1.3315953964421667 10 38.441485343563464 39.131722779316576 8.472442344437367 13.954349532682594 11 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18.564107121696882 24 21.526224578969977 34.44966154666509 25.541747515293352 18.482366359071587 25 23.54484025843163 23.58188607669966 28.217058858396065 24.656214806472647 26 24.65613327799846 23.314756625363135 25.782593229382815 26.246516867255586 27 20.670067926319224 24.122741968973394 25.18316376471984 30.024026339987543 28 21.34308214810091 28.67693950494609 27.653610571432807 22.326367775520193 29 25.643840493709945 25.67944457631142 27.036850225492525 21.639864704486115 30 19.18410383592973 33.604110667240896 29.95084426095609 17.260941235873297 31 23.53046009445451 29.649211393946594 31.040781774437875 15.779546737161018 32 17.634469331034587 29.624124964701338 36.79829971909695 15.943105985167131 33 19.5001932625695 30.157880652930157 32.01171468498201 18.33021139951833 34 17.559249457313626 31.843052127627942 34.53418180736864 16.06351660768979 35 16.590814444642753 35.78291391516449 34.616871913855675 13.009399726337081 36 17.925216123320485 32.26003243709732 34.520213668219085 15.294537771363101 37 15.407845906516219 31.75022912691377 38.325044698529084 14.516880268040927 38 16.2509864649563 31.747048050149644 38.26888428952188 13.733081195372176 39 15.833358148953277 31.52080045263691 36.950954408743044 15.694886989666776 40 15.430745133139403 32.71425374804207 38.40978593272171 13.445215186096815 41 13.792127298191245 33.71822759915373 38.390595667332164 14.099049435322863 42 14.795439302481556 32.63879573738729 36.89395633057605 15.671808629555109 43 15.017298973991888 32.98287998091148 36.652946790742064 15.34687425435457 44 14.838353548464097 31.992122812513983 38.1640782347939 15.005445404228022 45 15.68425606826896 33.231884706619525 37.215235196705905 13.868624028405616 46 15.14260555497515 33.15672432577646 36.17151470829664 15.529155410951748 47 15.89674723962996 33.572068039391226 37.11578633243808 13.415398388540734 48 17.10196031556299 34.02163519005498 35.60542672722926 13.270977767152761 49 15.800164289448137 34.80098573668882 36.45881562243298 12.940034351430066 50 14.930545182972368 35.13517550410754 36.48095593726662 13.453323375653472 51 14.855521541103561 35.0571193907265 37.02680504741282 13.060554020757111 52 15.560568935636168 34.766628817307115 36.41158190402199 13.261220343034722 53 14.310051107325384 35.172898173884455 36.14274186317583 14.374308855614334 54 16.676381353779014 34.02980167092618 35.46159709456127 13.832219880733534 55 15.879231746506239 35.3725431582094 34.50863164188028 14.239593453404082 56 16.307466969570157 35.911998565194 33.426496084175284 14.35403838106056 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 4.5 4 17.5 5 26.0 6 35.0 7 44.0 8 327.5 9 611.0 10 489.5 11 368.0 12 308.5 13 231.0 14 213.0 15 377.5 16 542.0 17 699.0 18 856.0 19 929.0 20 1002.0 21 1214.5 22 1677.5 23 1928.0 24 2236.5 25 2545.0 26 2963.0 27 3381.0 28 3739.0 29 4541.0 30 4985.0 31 4019.5 32 3054.0 33 3220.0 34 3386.0 35 2672.0 36 1958.0 37 1933.0 38 1839.0 39 1770.0 40 1764.0 41 1758.0 42 1978.5 43 2199.0 44 2103.5 45 2008.0 46 1998.0 47 2124.0 48 2260.0 49 2366.5 50 2473.0 51 2706.0 52 2939.0 53 3014.5 54 2896.0 55 2702.0 56 2820.5 57 2939.0 58 2654.0 59 2369.0 60 2165.0 61 1961.0 62 1661.5 63 1114.0 64 866.0 65 825.5 66 785.0 67 706.5 68 628.0 69 470.5 70 313.0 71 263.5 72 164.5 73 115.0 74 109.0 75 103.0 76 67.5 77 32.0 78 32.5 79 23.0 80 13.0 81 7.5 82 2.0 83 1.0 84 0.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.060729044776561554 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0014811962140624769 24 0.0 25 0.042954690207811834 26 0.06665382963281145 27 0.1288640706234355 28 0.12738287440937301 29 0.15552560247656005 30 0.2606905336749959 31 0.26513412231718336 32 0.3406751292343697 33 0.3658554648734318 34 0.37770503458593163 35 0.4102913512953061 36 0.4532460415031179 37 1.4160235806437278 38 0.5243434597781168 39 0.5198998711359293 40 0.7080117903218639 41 0.5850725045546784 42 0.6191400174781153 43 0.6769066698265519 44 0.7168989676062388 45 0.7168989676062388 46 0.7554100691718633 47 0.7302297335328011 48 0.866499785226549 49 0.8265074874468622 50 0.8339134685171745 51 0.8116955253062373 52 0.860575000370299 53 0.8813117473671738 54 0.894642513293736 55 0.9005672981499859 56 0.8961237095077986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 67513.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.45121680268985 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14485113520637 36.665531082902554 2 11.918169292517822 11.787359471509191 3 5.032049362008027 7.4652289188748835 4 2.6987359971245435 5.338231155481166 5 1.734259869406338 4.28806303971087 6 1.033367279698077 3.0660761631093267 7 0.7278499970047325 2.519514760120273 8 0.5091954711555742 2.0144268511249686 9 0.43131851674354527 1.91963029342497 >10 1.6264302402204518 13.71883933464666 >50 0.09584855927634338 3.3830521529186974 >100 0.04193374468340023 3.8733280997733774 >500 0.0 0.0 >1k 0.005990534954771461 3.9607186764030633 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 1442 2.1358849406780918 No Hit TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1232 1.8248337357249715 No Hit TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 480 0.7109741827499889 No Hit TACTTCGGGGTGGGGGCATGAGATGTATGTGAGAGGTTTATTACTGTGTGTGTGTG 313 0.4636144150015553 No Hit TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.3584494838031194 No Hit TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 204 0.3021640276687453 No Hit TACTTCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG 183 0.2710589071734333 No Hit TACTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 165 0.24439737532030867 No Hit TACTTCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 160 0.2369913942499963 No Hit TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 153 0.22662302075155896 No Hit TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.1984802926843719 No Hit TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.18959311539999704 No Hit TACTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121 0.1792247419015597 No Hit TACTTCGGGGCTTACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA 116 0.17181876083124734 No Hit TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.16145038733280997 No Hit TACTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.15848799490468501 No Hit TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 98 0.14515722897812275 No Hit TACTTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.14367603276406027 No Hit TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 92 0.13627005169374787 No Hit TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.1347888554796854 No Hit TACTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 90 0.1333076592656229 No Hit TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.1333076592656229 No Hit TACTTCGGGCAGTCTGTGCTGCATGTCTGTCACCCATACGCAGTCTCAATCATGGC 89 0.13182646305156043 No Hit TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 87 0.1288640706234355 No Hit TACTTCGGGATCACTGACGTGTCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCA 85 0.12590167819531053 No Hit TACTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.12293928576718557 No Hit TACTTCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG 79 0.11701450091093568 No Hit TACTTCGGGGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCTA 79 0.11701450091093568 No Hit TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.11257091226874824 No Hit TACTTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 75 0.11108971605468577 No Hit TACTTCGGGAGACTCTAATGATAAACCCAAGTCTATAGATATACCACTTGAAAGGG 74 0.1096085198406233 No Hit TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 73 0.10812732362656081 No Hit TACTTCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 73 0.10812732362656081 No Hit TACTTCGGGATGGCATTCTGGGAGGAATGGAGGTGGAGCAAGCCGTTTATTGACAC 70 0.10368373498437337 No Hit TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 69 0.1022025387703109 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0029623924281249538 0.0 0.0 0.0 0.0 8 0.0029623924281249538 0.0 0.0 0.0 0.0 9 0.0059247848562499076 0.0 0.0 0.0 0.0 10 0.031105120495312014 0.0 0.0 0.0 0.0 11 0.039992297779686874 0.0 0.0 0.0 0.0 12 0.04295469020781183 0.0 0.0 0.0 0.0 13 0.04591708263593678 0.0 0.0 0.0 0.0 14 0.05332306370624917 0.0 0.0 0.0 0.0 15 0.05480425992031164 0.0 0.0 0.0 0.0 16 0.05480425992031164 0.0 0.0 0.0 0.0 17 0.05628545613437412 0.0 0.0 0.0 0.0 18 0.06517263341874899 0.0 0.0 0.0 0.0 19 0.0770222031312488 0.0 0.0 0.0 0.0 20 0.09183416527187356 0.0 0.0 0.0 0.0 21 0.09775895012812347 0.0 0.0 0.0 0.0 22 0.13034526683749798 0.0 0.0 0.0 0.0 23 0.1466384251921852 0.0 0.0 0.0 0.0 24 0.16589397597499742 0.0 0.0 0.0 0.0 25 0.17626234947343475 0.0 0.0 0.0 0.0 26 0.18959311539999704 0.0 0.0 0.0 0.0 27 0.2058862737546843 0.0 0.0 0.0 0.0 28 0.24143498289218374 0.0 0.0 0.0 0.0 29 0.2488409639624961 0.0 0.0 0.0 0.0 30 0.26513412231718336 0.0 0.0 0.0 0.0 31 0.27846488824374566 0.0 0.0 0.0 0.0 32 0.29031445795624544 0.0 0.0 0.0 0.0 33 0.2992016352406203 0.0 0.0 0.0 0.0 34 0.3154947935953076 0.0 0.0 0.0 0.0 35 0.38659221187030646 0.0 0.0 0.0 0.0 36 0.3925169967265564 0.0 0.0 0.0 0.0 37 0.39992297779686875 0.0 0.0 0.0 0.0 38 0.4073289588671811 0.0 0.0 0.0 0.0 39 0.4221409210078059 0.0 0.0 0.0 0.0 40 0.4354716869343682 0.0 0.0 0.0 0.0 41 0.44435886421874304 0.0 0.0 0.0 0.0 42 0.4606520225734303 0.0 0.0 0.0 0.0 43 0.47842637714218 0.0 0.0 0.0 0.0 44 0.49620073171092977 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATAGGA 15 0.007984397 50.64027 35 CCATACG 15 0.007984397 50.64027 34 TTGTGCC 30 9.460964E-7 50.64027 36 CCAATAG 15 0.008032159 50.564007 33 TAAGAGA 15 0.008032159 50.564007 33 CCCATAC 15 0.008032159 50.564007 33 GCCAATA 15 0.00805612 50.525963 32 GGTAAAG 15 0.00805612 50.525963 32 ACAGCCC 30 1.012093E-6 50.148617 43 ACTGTGT 35 5.0697054E-8 50.148617 43 TCAGGAG 15 0.008298662 50.148617 45 ACGCAGT 15 0.008298662 50.148617 38 TTATTAC 35 5.0697054E-8 50.148617 38 TCAATCA 15 0.008298662 50.148617 46 TGATAAC 15 0.008298662 50.148617 48 ATTACTG 35 5.0697054E-8 50.148617 40 TATTACT 35 5.0697054E-8 50.148617 39 ATAACAC 15 0.008323211 50.111195 50 AACTCCT 15 0.008323211 50.111195 42 TCATGGC 15 0.008323211 50.111195 50 >>END_MODULE