FastQCFastQC Report
Fri 17 Jun 2016
SRR1526523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482663
Sequences flagged as poor quality0
Sequence length64
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43820.9078798250539196No Hit
ACAATAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG17220.35677066607550195No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.34454681630868744No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14380.29793044007931No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC11470.23763992682264853No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG9970.20656234266973025No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9220.19102355059327109No Hit
ACAATAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.17548475851681195No Hit
ACAATAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8110.16802613832011154No Hit
ACAATAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC8070.1671974027427004No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.16636866716528922No Hit
ACAATAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.16408964432740855No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.16098188591211673No Hit
ACAATAGGGCTGAGCTATTCCTTCATACTAAGAGTGACCTTGTTTCCACTTGGATGACAGTGGA7130.14772211667353827No Hit
ACAATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG7050.14606464551871595No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.13881320921636836No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.134255163540607No Hit
ACAATAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG6330.1311474051253152No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA6270.12990430175919845No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5720.11850918756979506No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5710.11830200367544227No Hit
ACAATAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA5200.10773562506345005No Hit
ACAATAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG5100.10566378611992218No Hit
ACAATAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4850.10048418876110246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA150.004624542358.1923953
CGTAAGC150.00462645158.18632552
AATCGCC150.004628360358.18026451
CGCCCGT150.004643655458.13180544
TTGCGTG150.004645569758.1257543
CACATCG150.00466090758.07738538
CGGTACA150.004674358358.0351332
TAAACGT150.004676282458.029131
CTAGACT150.00471875857.89672521
CGATATC150.00471875857.89672521
GCGTACG202.0352984E-457.84873615
GACGATA150.004734275457.84873219
AATACGC150.004736217757.84274310
TAGGGAT32550.055.4437375
AGGGATT8550.054.7983866
TAGGGTG17200.054.6479385
TAGGGGC48450.054.4999245
ATAGGGT36300.054.337124
ATAGGGG208100.054.28494
AATAAGG1600.054.2275733
Produced by FastQC (version 0.11.5)