FastQCFastQC Report
Fri 17 Jun 2016
SRR1526522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470548
Sequences flagged as poor quality0
Sequence length64
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.7495515866606595No Hit
ACAATAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG13410.28498686637707527No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.27074814896673666No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11710.2488587774254699No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8900.18914117157016924No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT8730.18552836267500872No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG7300.1551382643215995No Hit
ACAATAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.15386315529977815No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.14174961959247515No Hit
ACAATAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC6640.1411120650815645No Hit
ACAATAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.14089954691126091No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.13154874741790423No Hit
ACAATAGGGCTGAGCTATTCCTTCATACTAAGAGTGACCTTGTTTCCACTTGGATGACAGTGGA6170.13112371107729712No Hit
ACAATAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.1285734930336544No Hit
ACAATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG5690.12092283890272619No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.11135952123906594No Hit
ACAATAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG5190.11029693038754813No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.10902182136572677No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA5030.10689663966269117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT150.004641280558.1390756
CACGTAT150.00465112558.1079628
AGCGTTA150.004682731358.00865627
AAGCGTA150.004682731358.00865627
TACTAAG1000.057.9900726
CTCGATA150.004712507657.9158624
CGATTAA150.00473044157.86032513
GATATAC150.00476244857.761869
TAGGCGT150.00476244857.761865
TAGGGGC43000.055.343925
TAGGGTG18950.055.1709565
CATACTA1050.055.1579624
TAGGGGT39800.055.004385
ATAGGGT39700.054.7791944
TAGGGAT31800.054.7647825
ATAGGGG197250.054.716374
AATAGGT1850.054.6396033
TAGGGAG45450.054.3304635
AATAGGG484300.054.2732473
CAATAGG494650.054.2528272
Produced by FastQC (version 0.11.5)