FastQCFastQC Report
Fri 17 Jun 2016
SRR1526521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413753
Sequences flagged as poor quality0
Sequence length56
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT45791.1066989242374072No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.4118399141516798No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA16120.3896044258289366No Hit
ACAATAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA12640.30549627434725546No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC9820.23733966883623803No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.19746080390957893No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG7880.19045179128610548No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.18005911739612765No Hit
ACAATAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6780.16386588133499938No Hit
ACAATAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.1631408110636056No Hit
ACAATAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6640.16048222006849497No Hit
ACAATAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG6540.15806531916384897No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.15057292635944636No Hit
ACAATAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC5780.1396968722885393No Hit
ACAATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG5560.1343796902983181No Hit
ACAATAGGGCTGAGCTATTCCTTCATACTAAGAGTGACCTTGTTTCCACTTGGATG5530.13365462002692427No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5510.13317123984599508No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC5380.13002926866995526No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5080.12277856595601724No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.11939490468951283No Hit
ACAATAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC4540.1097273010709288No Hit
ACAATAGGGGTGTAAGGGTGTGTAAATGGTATATATGTGTGGTGTGTGTGTATGTG4540.1097273010709288No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4510.10900223079953499No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT4480.1082771605281412No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4310.10416842899024298No Hit
ACAATAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA4280.10344335871884916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGT150.00800644750.6519836
TTCCACG402.401066E-950.6146135
ATCCGTG150.00828658650.2132539
TCCGACG204.1037187E-450.1887644
CGCTTAG301.0193835E-650.1887644
ACGCTTA301.020242E-650.1826443
TACGCTT301.0228177E-650.1642942
TTAGCGA301.0541771E-649.94515230
CGGTATT204.2034438E-449.94515230
ATTAGCG252.10021E-549.93909529
TATTCCG150.00847193549.93303728
GCCGTAT150.00847193549.93303728
AAACGTA150.00848006449.92092527
ATGCAGG150.00848006449.92092527
ATTCGGT204.2135213E-449.9209227
CTATGCA150.00851263349.8725325
TAGACCT150.00851263349.8725325
TAAGGTG150.00853303649.84233524
CCGAACA150.00853303649.84233524
GTACTCT150.00856575849.79409422
Produced by FastQC (version 0.11.5)