##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413753
Sequences flagged as poor quality	0
Sequence length	56
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3349655470776	39.0	38.0	40.0	35.0	40.0
2	38.12489093734668	39.0	38.0	40.0	35.0	40.0
3	37.880564007995105	39.0	38.0	40.0	33.0	40.0
4	38.1178287529033	39.0	38.0	40.0	35.0	40.0
5	37.83496917242896	39.0	38.0	40.0	34.0	40.0
6	38.22245639306543	39.0	38.0	40.0	35.0	40.0
7	38.12978757857949	39.0	38.0	40.0	35.0	40.0
8	38.42234859928508	39.0	38.0	40.0	36.0	40.0
9	38.38639961522938	39.0	38.0	40.0	36.0	40.0
10	37.59897813429752	39.0	38.0	40.0	33.0	40.0
11	37.9213467938601	39.0	38.0	40.0	35.0	40.0
12	37.8363443890437	39.0	38.0	40.0	34.0	40.0
13	37.81775358728517	39.0	38.0	40.0	34.0	40.0
14	37.80650533047495	39.0	38.0	40.0	34.0	40.0
15	37.80095612599788	39.0	38.0	40.0	34.0	40.0
16	37.699753234417635	39.0	38.0	40.0	34.0	40.0
17	37.649834563133076	39.0	38.0	40.0	33.0	40.0
18	37.569888315009194	39.0	38.0	40.0	33.0	40.0
19	37.45751209054678	39.0	38.0	40.0	33.0	40.0
20	37.467356127931396	39.0	38.0	40.0	33.0	40.0
21	37.37326134191172	39.0	38.0	40.0	33.0	40.0
22	37.281955659536	39.0	37.0	40.0	33.0	40.0
23	37.090087564319774	39.0	37.0	40.0	33.0	40.0
24	36.986796470357916	39.0	36.0	40.0	33.0	40.0
25	36.91251060415272	39.0	36.0	40.0	32.0	40.0
26	36.56516810754242	39.0	36.0	40.0	32.0	40.0
27	36.44398469618347	39.0	36.0	40.0	31.0	40.0
28	36.259159450203384	38.0	36.0	40.0	31.0	40.0
29	36.19475145799547	38.0	36.0	40.0	30.0	40.0
30	36.00191901931829	38.0	35.0	40.0	30.0	40.0
31	35.498306960916295	38.0	35.0	40.0	29.0	40.0
32	35.28932479039427	38.0	35.0	40.0	28.0	40.0
33	35.2151090143153	38.0	35.0	40.0	28.0	40.0
34	35.35450135708986	38.0	35.0	40.0	28.0	40.0
35	35.18383673351009	38.0	35.0	40.0	28.0	40.0
36	34.701764095970304	38.0	35.0	40.0	26.0	40.0
37	34.231742126341075	38.0	34.0	40.0	23.0	40.0
38	34.27979495012725	38.0	34.0	40.0	23.0	40.0
39	34.21381597233132	38.0	34.0	40.0	23.0	40.0
40	33.82375958603321	38.0	33.0	40.0	20.0	40.0
41	33.383680601711646	38.0	33.0	40.0	17.0	40.0
42	33.246332957102425	38.0	33.0	40.0	16.0	40.0
43	33.089343158841146	38.0	32.0	39.0	15.0	40.0
44	32.58257946165949	38.0	32.0	39.0	9.0	40.0
45	32.55674762479064	37.0	32.0	39.0	4.0	40.0
46	32.01566876856482	37.0	31.0	39.0	4.0	40.0
47	31.777961730791077	37.0	31.0	39.0	4.0	40.0
48	31.39824242966214	36.0	31.0	39.0	4.0	40.0
49	31.255514763639177	36.0	30.0	39.0	4.0	40.0
50	30.920358281390104	36.0	30.0	39.0	4.0	40.0
51	30.240525144228563	36.0	30.0	39.0	4.0	40.0
52	29.996838693616724	36.0	29.0	39.0	4.0	40.0
53	29.615630581530528	35.0	28.0	39.0	4.0	40.0
54	29.17955156820615	35.0	27.0	39.0	4.0	40.0
55	28.639787506072462	35.0	24.0	39.0	4.0	40.0
56	17.00399997099719	20.0	4.0	24.0	4.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.9558307650393658
1	2	-0.7085093925353121
1	3	-0.9768675531837445
1	4	-0.802367432926161
1	5	-0.43400552155172534
1	6	-0.8296771190743897
1	7	-1.4747516408492274
1	8	-1.8471392945342942
1	9	-2.0098979708796563
1	10	-0.5662333753562336
1	11	-0.5675056259710303
1	12	-0.5462556843167761
1	13	-0.9519888654436386
1	14	-0.884904230679588
1	15	-0.7515562069382185
1	16	-0.8144358115495862
1	17	-0.7100432698399572
1	18	-1.1630426980581134
1	19	-0.7685910183863882
1	20	-1.0521795974589594
1	21	-0.7516624566942767
1	22	-0.8117685581614325
1	23	-0.8252850924026518
1	24	-0.6608580966406592
1	25	-0.9065208589197127
1	26	-0.7988999397311503
1	27	-0.9462273654015334
1	28	-0.8501698101896267
1	29	-0.8173255799464343
1	30	-0.605091553537541
1	31	-0.4809644457095814
1	32	-0.583726312129123
1	33	-0.45846760027097133
1	34	-0.9089528158574893
1	35	-0.598154683230085
1	36	-0.5178173345150441
1	37	-0.6713438406047487
1	38	-0.2537086656611791
1	39	-0.8633750279653043
1	40	-0.42098573382907034
1	41	-0.4539074556682081
1	42	-0.6563657809091978
1	43	-0.4075860478354727
1	44	0.01864250911820875
1	45	-0.5510301700499625
1	46	-0.03363568866328848
1	47	-0.24635213011253754
1	48	0.432090523176349
1	49	0.4604284367119753
1	50	0.269041256897264
1	51	-0.06795462191878343
1	52	0.4252621630614044
1	53	0.12829790248447992
1	54	0.20954137206137702
1	55	0.585532100199714
1	56	0.732389718899114
2	1	-0.17315796714892429
2	2	0.3576675556220792
2	3	-0.24064656933015982
2	4	-0.22021942622338742
2	5	0.049321966335213574
2	6	-0.14860467450870374
2	7	0.20380726176563257
2	8	-0.06288822611659128
2	9	-0.12223446075404354
2	10	0.0764864459030079
2	11	-0.3045904369033181
2	12	-0.35823365347879843
2	13	-0.3139973608257307
2	14	-0.32152010691200417
2	15	-0.7385013766510156
2	16	-0.6902395604292124
2	17	-0.6550895787653062
2	18	-0.42434714189636225
2	19	-0.6629962766901727
2	20	-0.811613498450086
2	21	-0.736009650230514
2	22	-0.6290139240214287
2	23	-0.4585287955578963
2	24	-0.9882355695892286
2	25	-0.472821210945483
2	26	-0.38830279490696995
2	27	-0.49742698090106074
2	28	-0.7353587480070658
2	29	-0.6935709847760734
2	30	-0.7264754954467918
2	31	-0.3593401910040015
2	32	-0.7439305130566041
2	33	-0.35063210412246804
2	34	-0.5538549371050365
2	35	-0.42372656273595055
2	36	-0.14875533381358963
2	37	-0.1798652024638585
2	38	-0.03621909004058921
2	39	-0.27271864928429324
2	40	0.12143921713413164
2	41	0.20612138186735507
2	42	0.050666940600748944
2	43	-0.08384574153408408
2	44	0.3295358232265144
2	45	0.23390936205452562
2	46	0.06607463699302585
2	47	0.20742337876604466
2	48	-0.039039466561643366
2	49	0.18937508727119123
2	50	-0.22980913864460817
2	51	-0.1764006424687743
2	52	0.18320222775101058
2	53	-0.023826592533900737
2	54	0.24993340666641828
2	55	0.3711471061101079
2	56	-0.3209727117673964
3	1	0.0446200492439317
3	2	0.5104110324823807
3	3	-0.022725383890453088
3	4	-0.0285697608810338
3	5	0.16199600620779364
3	6	0.12398919069763537
3	7	0.40465553836823176
3	8	0.1633546601713931
3	9	0.10944884931431886
3	10	0.11730752038670289
3	11	-0.15143534903331357
3	12	-0.12635348597100915
3	13	-0.26082795605104536
3	14	-0.22437990862287194
3	15	-0.23730233588362637
3	16	-0.4565518837049183
3	17	-0.25212240026930033
3	18	-0.2676128278550607
3	19	-0.37640763715403835
3	20	-0.41440887408987237
3	21	-0.3318792232288317
3	22	-0.3591760629669878
3	23	-0.3700503246033122
3	24	-0.3851538558557408
3	25	-0.303112202033212
3	26	-0.38150226518149566
3	27	-0.29720506887844067
3	28	-0.2392601045337912
3	29	-0.29019219527036455
3	30	-0.33209845976190877
3	31	-0.24691669696341023
3	32	-0.22927358188512414
3	33	-0.2873334892830002
3	34	-0.04368993477854133
3	35	0.030405653878815997
3	36	-0.2826362887511351
3	37	0.16830402334952055
3	38	-0.03704231205998809
3	39	-0.026306823521025535
3	40	-0.20378716892120252
3	41	0.09326838068620447
3	42	-0.17312417575989514
3	43	-0.0292088540291644
3	44	0.1267189722297033
3	45	0.24507654770899023
3	46	0.16955006560536034
3	47	0.07892915921631172
3	48	0.15638628239143415
3	49	-0.0038852298482474623
3	50	0.07299339544752215
3	51	0.6489152242408416
3	52	0.5631549819655497
3	53	0.8411292040228808
3	54	0.57759366651824
3	55	0.993431368252633
3	56	-0.40257906847307723
4	1	-0.2796551840347874
4	2	0.3993157502922884
4	3	-0.25785015536040845
4	4	-0.025857079540955397
4	5	0.18314542330777783
4	6	-0.07907607985819709
4	7	0.35125073745648905
4	8	0.1413504813362394
4	9	0.0898486878593232
4	10	0.14101528684680176
4	11	-0.04737404362219877
4	12	-0.15486928719142412
4	13	-0.10585490478908355
4	14	-0.1404945416689074
4	15	-0.03553107962131463
4	16	-0.3188046557447137
4	17	-0.3605228086554604
4	18	-0.36300779900685143
4	19	-0.24847236161209452
4	20	-0.42114012275430923
4	21	-0.320820743280116
4	22	-0.08016165073439652
4	23	-0.5630187221624965
4	24	-0.3049071621216157
4	25	-0.1308183539433685
4	26	-0.042482391971155664
4	27	-0.16525122601213837
4	28	0.19050697289338103
4	29	-0.46808948140185436
4	30	0.022354611444498573
4	31	-0.14931359132910416
4	32	-0.09042822252904159
4	33	-0.12921443996799553
4	34	0.21998625298412122
4	35	-0.33779483523484544
4	36	-0.2475435708462541
4	37	-0.24416006478591612
4	38	-0.3633950912227988
4	39	-0.10629827590367569
4	40	-0.03652098187084363
4	41	0.10445151338796421
4	42	0.1659075691412113
4	43	0.3050267278023995
4	44	0.5258808308616096
4	45	0.07422511004723731
4	46	0.8012724105017206
4	47	0.6410596528412178
4	48	0.685050479746419
4	49	0.9131079083144904
4	50	0.8762201209977754
4	51	0.5368523992533092
4	52	0.8934135473904377
4	53	1.2806904263321641
4	54	0.4687896206460245
4	55	1.0300555342663564
4	56	1.1587204067981922
5	1	-0.20336227555612396
5	2	0.3162881581929753
5	3	-0.03302983068834919
5	4	0.12219287773903886
5	5	0.3380054524578995
5	6	0.1449024093612863
5	7	0.47469899598792153
5	8	0.1654118976301575
5	9	0.1931808484865556
5	10	0.0656270373619563
5	11	0.0947453713649864
5	12	-0.2583270949011265
5	13	-0.160084185585184
5	14	0.027369960517347636
5	15	-0.012613051777918827
5	16	-0.07917054004887802
5	17	0.12404772961664179
5	18	-0.1327846497883769
5	19	-0.42155121772105986
5	20	-0.22533170942566016
5	21	-0.20795196909305247
5	22	-0.1720222327316847
5	23	-0.2025498072506977
5	24	-0.11579573828888101
5	25	-0.26641668535010865
5	26	-0.25593165567924814
5	27	-0.3319346456231358
5	28	-0.2180973393144896
5	29	-0.4229570493769259
5	30	-0.5313218221887155
5	31	-0.30960157444214076
5	32	-0.40651858791076023
5	33	-0.4491370413651907
5	34	-0.44351829544026344
5	35	0.08930472751330853
5	36	-0.5303751534969052
5	37	-2.7562598788282315
5	38	-0.48617742021711763
5	39	-0.15072708426674808
5	40	-0.44388138796221455
5	41	-0.17383164487709735
5	42	-0.47529462214390605
5	43	-0.28541404216291966
5	44	0.3211766040937505
5	45	0.1883503550590504
5	46	0.5176492512314788
5	47	0.23088475210240844
5	48	0.3692616673626432
5	49	0.24127295832202833
5	50	0.383532781197804
5	51	0.555536120417301
5	52	0.2575639218187433
5	53	0.435311726025585
5	54	0.16193431582620477
5	55	0.38435517742864533
5	56	0.5821133806133396
6	1	-0.07809577809419466
6	2	0.42310287900516386
6	3	-0.003270947439617089
6	4	-0.3515253963726366
6	5	0.12666027479292552
6	6	-0.07882855510572284
6	7	0.4623893513178743
6	8	0.11833067935604191
6	9	0.14106155914645058
6	10	0.20131231654977455
6	11	-0.424378994117248
6	12	-0.0311316634290435
6	13	-0.21422124142274868
6	14	-0.374355927807521
6	15	-0.21850137665101244
6	16	-0.3778888141605563
6	17	-0.2451020165762543
6	18	-0.796299878214775
6	19	-0.521665430919029
6	20	-0.40599160790281985
6	21	-0.3078504462504128
6	22	-0.5133547200413275
6	23	-0.4579939696872444
6	24	-0.5058972611315156
6	25	-0.26324409651762437
6	26	0.28241859812785464
6	27	-0.03792449333887049
6	28	0.3120168738834792
6	29	0.3961926968159659
6	30	0.3397060965930123
6	31	0.21603294332435752
6	32	0.33603217351056003
6	33	0.6607360550815073
6	34	0.1820654609049157
6	35	0.26198239248792987
6	36	0.28352078558939553
6	37	-0.20742739151858558
6	38	0.6167286711534388
6	39	0.674196773601274
6	40	0.6209790181291552
6	41	0.48130794903153173
6	42	0.5467739057748773
6	43	0.3658930644360652
6	44	0.5437273655150747
6	45	0.6326904565818907
6	46	0.8285248857492427
6	47	0.9319507419501285
6	48	1.1596296876672092
6	49	0.8634049380174602
6	50	-0.0818986908834134
6	51	0.6607137853919234
6	52	0.7983887949151907
6	53	0.12111121841137162
6	54	0.4813886305470163
6	55	0.24064959367229832
6	56	-0.3854627615186388
7	1	-0.20574691159545466
7	2	0.3234996044459635
7	3	-0.2371815267847026
7	4	-0.17061229813586465
7	5	-0.08584602243629291
7	6	-0.01566734603770925
7	7	0.31988305408865614
7	8	0.038418840565114465
7	9	0.05861193698020628
7	10	-0.003297255238642549
7	11	-0.005569170439663651
7	12	-0.20432435864314868
7	13	-0.10927161925650353
7	14	-0.0566229303264123
7	15	-0.2397608224948442
7	16	-0.09363818443762995
7	17	-0.34781612236970716
7	18	-0.5262432031518003
7	19	-0.3737498137905675
7	20	-0.19417800588770717
7	21	-0.3602937711874432
7	22	-0.2255902364141278
7	23	-0.4659977480247761
7	24	-0.17504083795771663
7	25	-0.21570001591309307
7	26	0.08958484497924246
7	27	0.5265087560314043
7	28	0.07667052627642335
7	29	0.18366750789909503
7	30	0.13544287241165165
7	31	0.1655291649868289
7	32	0.16907373522340663
7	33	0.45256225155506513
7	34	0.6058941762701551
7	35	-0.015139773758924946
7	36	0.4136530274029937
7	37	0.8905071172975312
7	38	0.6198206107000388
7	39	0.7006199474048032
7	40	0.8331263732928846
7	41	1.3589842714496712
7	42	1.375873401996536
7	43	1.4998792105317804
7	44	1.4635195569508426
7	45	0.8882698016700488
7	46	0.937485842928087
7	47	1.1760061575672545
7	48	1.016802231747814
7	49	0.8632538045162015
7	50	0.9739955156657061
7	51	0.976280536021644
7	52	0.7932439586431492
7	53	0.7707270874290053
7	54	0.5583848522094854
7	55	0.2817327170979915
7	56	0.04681053822947234
8	1	0.25440422190580136
8	2	0.680602879005157
8	3	0.17172905256038717
8	4	0.3259746036273583
8	5	0.7416602747929204
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114	3	0.0929025219481403
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115	3	0.08922905256038405
115	4	0.10097460362736399
115	5	0.5141602747929213
115	6	-0.1563285551057234
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115	13	-0.14672124142274612
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115	53	0.25611121841136963
115	54	-0.056111369452985116
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116	1	-0.018929111427532064
116	2	0.4472197944280438
116	3	-0.011790847937128035
116	4	0.16784027526914969
116	5	0.4125060459371994
116	6	-0.26303253520522674
116	7	-2.365844479527901
116	8	-0.030549917658881043
116	9	-0.2018364508037962
116	10	0.041660575256237564
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116	12	-0.3607212156678514
116	13	-0.4358879080894127
116	14	-0.4085847835289158
116	15	-0.23850137665101556
116	16	0.2998101908145685
116	17	0.2056069386476267
116	18	0.030876738700648332
116	19	0.0061579521655517055
116	20	0.20828699906235215
116	21	-0.019591739782754303
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116	30	0.25362400704077004
116	31	-0.10312128553136546
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116	34	0.22475202806909067
116	35	0.4767709497018586
116	36	-0.1089543387887133
116	37	0.4246124094764383
116	38	0.4463679746360256
116	39	-0.08117636072708478
116	40	0.18114070967144613
116	41	-0.3112915534560301
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116	43	-0.02663181118582969
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116	46	0.03871145291342515
116	47	0.010905965830723119
116	48	0.2942938667716888
116	49	-0.09669456447010205
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116	53	0.446322661197442
116	54	0.1255552972136833
116	55	0.15721675785140476
116	56	0.09445017380474141
117	1	-0.05073572720360886
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117	3	-0.018569929627908266
117	4	0.030796486579014015
117	5	0.23560429514915882
117	6	-0.414261125080273
117	7	-3.1856386385040096
117	8	-0.39827237407907035
117	9	-0.33996261388153926
117	10	0.07696117151160564
117	11	-0.18784591523684213
117	12	-0.10433140897612247
117	13	-0.04148587246600499
117	14	-0.3014297191561255
117	15	-0.40516804331767986
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117	17	-0.12079540080016926
117	18	-0.04525025989416065
117	19	-0.281582733718011
117	20	-0.003421633348111186
117	21	-0.05741787627586348
117	22	0.10810202296121219
117	23	-0.13674078902566578
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117	25	-0.01038150109777547
117	26	-0.2399605367321911
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117	28	-0.37333299889005644
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117	30	-0.18925700773269227
117	31	0.07239426647957714
117	32	0.10449909463015672
117	33	0.27777167849117745
117	34	-0.1157971345149349
117	35	0.2856274306558717
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117	37	0.9776107764203417
117	38	0.581607806013487
117	39	0.541397791412983
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117	41	0.050487338344510135
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117	44	0.8398278744209264
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117	46	1.0276788297696022
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117	53	0.7863211420754936
117	54	0.7680489358905263
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117	56	-0.1621485121547721
118	1	0.09121220195567759
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118	3	0.2119098505653696
118	4	0.43498956622087803
118	5	0.7138298508527754
118	6	0.37996196858505016
118	7	0.6631998251333329
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118	11	0.2254402078777673
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118	13	0.011327387006176082
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118	15	0.1767105934237989
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118	17	0.23019100088010447
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118	20	0.3620133796283511
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118	23	0.39834642931524655
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118	34	-0.10576496303523442
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118	37	0.3088818354140841
118	38	0.09368004272451458
118	39	0.08858580103269986
118	40	-0.19799230356660757
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118	42	-0.05601287726751991
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118	44	0.42389569469212773
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118	47	0.1273871509276816
118	48	0.5151596128542444
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118	50	-0.15603834175623277
118	51	0.1974718901300747
118	52	-0.1433880130648575
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118	54	0.01125770785374769
118	55	0.208804207138634
118	56	-0.16984555453609573
119	1	0.02138676554670127
119	2	0.41773504858122124
119	3	0.01739613485464986
119	4	0.06840602507374882
119	5	0.5317849630722264
119	6	0.1679918937720828
119	7	0.4886362341108921
119	8	0.27188429531614133
119	9	0.26220245690205957
119	10	0.5813871295173527
119	11	0.19302125525931046
119	12	0.2862062418078608
119	13	0.08863412017326056
119	14	-0.15086465598707122
119	15	0.21910460838638812
119	16	-0.06687260468424938
119	17	-0.12392371233685395
119	18	0.08964151829395917
119	19	-0.025175405981372023
119	20	-0.3013282662569381
119	21	0.08102735923586835
119	22	-0.2034981115625243
119	23	-0.04803761058499845
119	24	-0.2401865379395005
119	25	-0.045351328437824634
119	26	-0.315623795887106
119	27	-0.08869756067053913
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119	29	0.08311913073118404
119	30	0.019749737490762698
119	31	-0.007533141463667903
119	32	0.040340153560435965
119	33	0.384464234632631
119	34	0.2857562339722435
119	35	-0.3747445401804015
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119	37	0.3886823341671999
119	38	-0.2155468899438162
119	39	-0.3353543485932349
119	40	-0.16806711653418205
119	41	-0.041915242988416423
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119	44	-0.10478260954727148
119	45	0.05375030695595484
119	46	-0.20078309430062902
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119	48	0.048825448265716886
119	49	-0.15150778018702837
119	50	-0.013893703352241715
119	51	0.1276464537210984
119	52	-0.20323838712969788
119	53	-0.017623195553717608
119	54	0.6272178076043708
119	55	0.3584301423007261
119	56	-0.17483308570816547
120	1	-0.31059577809419636
120	2	0.10223553206638769
120	3	-0.2285260494804291
120	4	-0.32382131473998754
120	5	-0.048441766023401556
120	6	-0.5043387591873554
120	7	-0.06873309766172042
120	8	-0.32707748390926383
120	9	-0.4855200735066134
120	10	-0.5876672752869609
120	11	-0.5203483818723527
120	12	-0.5158765613882323
120	13	-0.7079967516268297
120	14	-0.8538457237258896
120	15	-0.7461544378755036
120	16	-0.8797765692625958
120	17	-0.9357652818823752
120	18	-0.9241060006637483
120	19	-0.8832470635720924
120	20	-1.1706854854538449
120	21	-0.7709116707402117
120	22	-0.809324107796435
120	23	-0.7772286635647987
120	24	-0.8359993019478438
120	25	-0.4214073618237464
120	26	-0.5861528304435737
120	27	-0.23465918721642254
120	28	-0.5727280240757082
120	29	-0.6769195480819903
120	30	-0.585242882998827
120	31	-0.3574364444307463
120	32	-0.24156986730576335
120	33	-0.6191619041021639
120	34	-0.23028147787059794
120	35	-0.7313339340426808
120	36	-0.3514281940024446
120	37	-0.36130494253899315
120	38	-0.5033223492547236
120	39	-0.35223179782729375
120	40	-0.2865209818708436
120	41	0.048909989847857105
120	42	0.02570247720344554
120	43	0.06022979912994231
120	44	0.38408450837221864
120	45	0.43177208923495414
120	46	0.009698355137000902
120	47	-0.31284517641721976
120	48	0.017946014197825377
120	49	0.19462942781337844
120	50	-0.02618440516912912
120	51	0.4109178670245761
120	52	0.14843981532335349
120	53	0.13657040208483906
120	54	0.07878658973069008
120	55	-0.05230959000116897
120	56	-0.6857178635594572
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	367.0
5	37.0
6	74.0
7	113.0
8	264.0
9	383.0
10	318.0
11	483.0
12	528.0
13	617.0
14	930.0
15	1068.0
16	1295.0
17	1534.0
18	1884.0
19	2297.0
20	2251.0
21	2845.0
22	3113.0
23	3265.0
24	4348.0
25	5444.0
26	5883.0
27	5825.0
28	7019.0
29	7697.0
30	8931.0
31	11656.0
32	14080.0
33	17172.0
34	22670.0
35	29369.0
36	39890.0
37	60801.0
38	78209.0
39	71093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.47161854002277	0.3097742106319539	0.1516225059065067	0.06698474343875974
8	99.4825415163153	0.42537455921769746	0.042054075740840545	0.050029848726172374
9	97.72448779827579	1.2959422650711898	0.390571186190795	0.5889987504622324
10	41.05227031586478	37.33120968307178	7.837284563495612	13.779235437567824
11	27.491281029986492	33.82887858214925	19.54861958704831	19.131220800815946
12	38.248906956565875	19.324814563278093	22.713793011772722	19.712485468383314
13	24.80006187266316	18.396241235713095	28.520639125275228	28.283057766348524
14	24.86846016826464	25.243804878756166	27.09104224017711	22.796692712802084
15	30.006791491542057	21.54570480455731	27.172733490754148	21.27477021314649
16	26.369113939959348	24.27752789707869	26.213948901881075	23.139409261080885
17	30.214161589160682	23.058443080775245	21.2931386600218	25.43425667004227
18	27.995204868605185	24.16973411673148	24.394747590954026	23.440313423709313
19	24.103269341853714	24.614443883186347	25.965491488883462	25.316795286076477
20	22.73457835955268	27.459861318226093	23.529980447271683	26.275579874949546
21	23.754027161132367	22.71620991267737	28.108557521033077	25.42120540515718
22	24.758974557284176	23.852878408132387	24.96561958463141	26.422527449952028
23	25.378851963746225	27.403987915407857	25.198791540785496	22.018368580060425
24	22.879703009781245	29.980350500663445	24.803807120707866	22.336139368847448
25	24.68921457916716	25.09424518013585	25.523214903191644	24.69332533750535
26	24.73881499395405	23.69286577992745	27.919709794437725	23.64860943168077
27	21.685560462161998	23.430403484362714	29.00151231020507	25.88252374327022
28	22.286207889432767	25.500197199524788	30.12289280814543	22.09070210289702
29	24.44460581415051	23.214242490257302	30.84743301140077	21.493718684191418
30	21.179292134014872	25.705564572785196	33.29053513893263	19.824608154267302
31	22.778695633215275	23.419202612415756	34.619111526703136	19.18299022766583
32	21.424398096406396	22.55220215136139	38.06969014480597	17.953709607426248
33	22.57095328625747	22.682499806006053	36.196845658415455	18.54970124932102
34	19.628339703913543	24.10165117677875	40.13125982265857	16.13874929664914
35	18.51301980990641	27.681226923618084	38.705248193916816	15.100505072558695
36	20.12006911011027	24.53093453952477	38.23041233110732	17.11858401925765
37	18.94495806036858	24.832425151533705	39.43255984816017	16.79005693993755
38	19.429530282830783	24.424418590532806	40.377998110737956	15.768053015898456
39	19.492138738992534	23.144455831710243	39.4755759339042	17.887829495393014
40	18.763794760728583	23.94149926387385	39.94451677272561	17.350189202671956
41	17.115251897860592	25.29865181426725	40.160042379883166	17.426053907988997
42	16.971812498176902	23.277052320437157	40.72141141695917	19.02972376442677
43	18.177196963616417	23.341773137101814	41.25531744120716	17.225712458074604
44	18.290929351568224	23.034644178809607	40.65770169364512	18.016724775977053
45	17.872202715953854	24.63264546363683	39.244340951727466	18.250810868681853
46	18.10142327531018	24.64771075890977	38.88070728044147	18.370158685338584
47	17.744318085026126	25.109939183614866	40.186513315600294	16.95922941575872
48	18.861008753972435	26.083767132635355	38.054921916125636	17.000302197266574
49	18.649605426332037	26.112761089846824	38.350757596060845	16.886875887760297
50	16.870023681671555	26.449162370506084	38.97778990556568	17.70302404225668
51	16.64385063210971	27.415536014420383	39.26533992643656	16.675273427033346
52	18.45005410358643	27.127099560347435	37.625632427057646	16.79721390900849
53	18.690841546145727	26.68191003649146	37.515021950940074	17.11222646642274
54	18.79711346238237	27.579842995757957	36.54200594860793	17.081037593251743
55	19.044472619105672	27.233627541814993	35.97990929926367	17.74199053981567
56	18.15677325609744	27.32024614064344	35.277395824190826	19.24558477906829
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.5
2	15.0
3	14.5
4	27.0
5	40.0
6	354.0
7	668.0
8	730.0
9	792.0
10	866.5
11	941.0
12	1245.0
13	2664.0
14	3779.0
15	5172.5
16	6566.0
17	7300.5
18	8035.0
19	9367.5
20	10700.0
21	12582.0
22	15580.5
23	16697.0
24	18004.5
25	19312.0
26	19918.0
27	20524.0
28	19799.5
29	16390.5
30	13706.0
31	14057.5
32	14409.0
33	11742.5
34	9076.0
35	8523.5
36	7971.0
37	7975.5
38	8286.5
39	8593.0
40	9336.0
41	10079.0
42	10665.0
43	11251.0
44	11139.0
45	11027.0
46	11369.0
47	12681.0
48	13651.0
49	15224.5
50	16798.0
51	16872.5
52	16947.0
53	16752.0
54	17922.5
55	19288.0
56	20375.5
57	21463.0
58	20181.5
59	18900.0
60	17148.5
61	15397.0
62	13762.5
63	10899.5
64	9671.0
65	9098.0
66	8525.0
67	6766.5
68	5008.0
69	4344.5
70	3681.0
71	3296.5
72	2217.5
73	1523.0
74	1260.0
75	997.0
76	808.5
77	620.0
78	498.5
79	276.5
80	176.0
81	132.0
82	88.0
83	66.0
84	44.0
85	32.0
86	20.0
87	13.5
88	3.5
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0546219604449998
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	7.250702713938026E-4
24	4.8338018092920174E-4
25	0.04930477845477857
26	0.06114759288754402
27	0.11552786324207923
28	0.11262758215650401
29	0.15057292635944636
30	0.2327475571174106
31	0.2308140363936938
32	0.3069464148900431
33	0.3308737338460386
34	0.34827542035948983
35	0.3709942888631623
36	0.40048047989984364
37	1.3118938110418537
38	0.4717790565869009
39	0.4802382097531619
40	0.6810826749292452
41	0.5406607323693122
42	0.5715970639487811
43	0.6298443757507498
44	0.663680988415794
45	0.6704483109488028
46	0.7105688659659265
47	0.6873666172813249
48	0.8277885598412579
49	0.7999941994378288
50	0.7997525093473642
51	0.7789671615674086
52	0.8287553202031164
53	0.8507491184353949
54	0.8635586932300189
55	0.8732262968486029
56	0.8654922139537357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	413753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.77969323962326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.71590781987874	28.429933348165076
2	12.451162052319908	10.155091379408878
3	5.697817735042249	6.970657781109238
4	3.213958887809586	5.242570301185428
5	2.1386301451897003	4.3606340636923475
6	1.4559237012450459	3.562327315024193
7	1.0037170810936433	2.8651892266458185
8	0.7426293520306487	2.422735773324004
9	0.561528547081709	2.0609065705755096
>10	2.801628672372912	19.830135499710465
>50	0.1311113518994986	3.6844847167274457
>100	0.07439806944994805	5.673201258281805
>500	0.009147303620895253	2.4644467833942354
>1k	0.002439280965572067	2.2776859827555804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	4579	1.1066989242374072	No Hit
ACAATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1704	0.4118399141516798	No Hit
ACAATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1612	0.3896044258289366	No Hit
ACAATAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	1264	0.30549627434725546	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	982	0.23733966883623803	No Hit
ACAATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.19746080390957893	No Hit
ACAATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	788	0.19045179128610548	No Hit
ACAATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	745	0.18005911739612765	No Hit
ACAATAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	678	0.16386588133499938	No Hit
ACAATAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	675	0.1631408110636056	No Hit
ACAATAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	664	0.16048222006849497	No Hit
ACAATAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	654	0.15806531916384897	No Hit
ACAATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	623	0.15057292635944636	No Hit
ACAATAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC	578	0.1396968722885393	No Hit
ACAATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	556	0.1343796902983181	No Hit
ACAATAGGGCTGAGCTATTCCTTCATACTAAGAGTGACCTTGTTTCCACTTGGATG	553	0.13365462002692427	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	551	0.13317123984599508	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	538	0.13002926866995526	No Hit
ACAATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	508	0.12277856595601724	No Hit
ACAATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	494	0.11939490468951283	No Hit
ACAATAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	454	0.1097273010709288	No Hit
ACAATAGGGGTGTAAGGGTGTGTAAATGGTATATATGTGTGGTGTGTGTGTATGTG	454	0.1097273010709288	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	451	0.10900223079953499	No Hit
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT	448	0.1082771605281412	No Hit
ACAATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	431	0.10416842899024298	No Hit
ACAATAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	428	0.10344335871884916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.007975772985331829	0.0	0.0	0.0	0.0
7	0.008700843256725632	0.0	0.0	0.0	0.0
8	0.010392673889977838	0.0	0.0	0.0	0.0
9	0.010634363980442438	0.0	0.0	0.0	0.0
10	0.023202248684601683	0.0	0.0	0.0	0.0
11	0.03649520366015473	0.0	0.0	0.0	0.0
12	0.03963717483619454	0.0	0.0	0.0	0.0
13	0.040362245107588346	0.0	0.0	0.0	0.0
14	0.048338018092920175	0.0	0.0	0.0	0.0
15	0.05220505954035379	0.0	0.0	0.0	0.0
16	0.056797171259181205	0.0	0.0	0.0	0.0
17	0.06066421270661482	0.0	0.0	0.0	0.0
18	0.06525632442544223	0.0	0.0	0.0	0.0
19	0.07009012623473425	0.0	0.0	0.0	0.0
20	0.08072449021517669	0.0	0.0	0.0	0.0
21	0.0850749118435395	0.0	0.0	0.0	0.0
22	0.09643434609537574	0.0	0.0	0.0	0.0
23	0.10465180917117217	0.0	0.0	0.0	0.0
24	0.11359434251836241	0.0	0.0	0.0	0.0
25	0.11842814432765442	0.0	0.0	0.0	0.0
26	0.12447039658926945	0.0	0.0	0.0	0.0
27	0.13099602903181368	0.0	0.0	0.0	0.0
28	0.15298982726409235	0.0	0.0	0.0	0.0
29	0.15806531916384897	0.0	0.0	0.0	0.0
30	0.1650743317873224	0.0	0.0	0.0	0.0
31	0.1694247534156852	0.0	0.0	0.0	0.0
32	0.17522531558683563	0.0	0.0	0.0	0.0
33	0.17981742730566305	0.0	0.0	0.0	0.0
34	0.18682643992913647	0.0	0.0	0.0	0.0
35	0.21413742015163637	0.0	0.0	0.0	0.0
36	0.22017967241325137	0.0	0.0	0.0	0.0
37	0.22525516431300802	0.0	0.0	0.0	0.0
38	0.23202248684601684	0.0	0.0	0.0	0.0
39	0.23878980937902566	0.0	0.0	0.0	0.0
40	0.2503909337213265	0.0	0.0	0.0	0.0
41	0.25836670670665834	0.0	0.0	0.0	0.0
42	0.2687593805966362	0.0	0.0	0.0	0.0
43	0.2777019139438264	0.0	0.0	0.0	0.0
44	0.28543599683869364	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCGT	15	0.008006447	50.65198	36
TTCCACG	40	2.401066E-9	50.61461	35
ATCCGTG	15	0.008286586	50.21325	39
TCCGACG	20	4.1037187E-4	50.18876	44
CGCTTAG	30	1.0193835E-6	50.18876	44
ACGCTTA	30	1.020242E-6	50.18264	43
TACGCTT	30	1.0228177E-6	50.16429	42
TTAGCGA	30	1.0541771E-6	49.945152	30
CGGTATT	20	4.2034438E-4	49.945152	30
ATTAGCG	25	2.10021E-5	49.939095	29
TATTCCG	15	0.008471935	49.933037	28
GCCGTAT	15	0.008471935	49.933037	28
AAACGTA	15	0.008480064	49.920925	27
ATGCAGG	15	0.008480064	49.920925	27
ATTCGGT	20	4.2135213E-4	49.92092	27
CTATGCA	15	0.008512633	49.87253	25
TAGACCT	15	0.008512633	49.87253	25
TAAGGTG	15	0.008533036	49.842335	24
CCGAACA	15	0.008533036	49.842335	24
GTACTCT	15	0.008565758	49.794094	22
>>END_MODULE