FastQCFastQC Report
Fri 17 Jun 2016
SRR1526520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68253
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4710.690079556942552No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.646125445035383No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG3100.4541924897074121No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.42049433724524926No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2680.3926567330373757No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2350.3443072099394898No Hit
AGCGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.26958521969730265No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1790.2622595343794412No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1610.23588706723513983No Hit
AGCGAGGGGCATTCAACCCAGGACTGAAAGAGGCTCTCTGTCCCTTTTGACAGAAAGGTCAAGT1500.21977055953584457No Hit
AGCGAGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1400.2051191889001216No Hit
AGCGAGGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCACATTATTGACAGTCTTGCATTTTG1340.19632836651868785No Hit
AGCGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1290.18900268120082633No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC1220.17874672175582026No Hit
AGCGAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1140.16702562524724188No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1090.1596999399293804No Hit
AGCGAGGGGAGACTAAGGATTTTGGATCCCAGGAGGCCTCAAAAGAAGAAAAAGAAGATGTGAT1090.1596999399293804No Hit
AGCGAGGGGGGGATCCTGGCTGCACAACTGTTAGATCTGGTTGTGAGGCATCCGGTTGATATCT1040.1523742546115189No Hit
AGCGAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG940.13772288397579593No Hit
AGCGAGGGGATGCGAGGGGTTAATTTTGCCCAGAGCTTTGCTATCTTTATTTCCATAGTGTTGG940.13772288397579593No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC930.13625774691222364No Hit
AGCGAGGGGGGTTTAATATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACCAACATAGA910.13332747278507903No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC890.13039719865793445No Hit
AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.13039719865793445No Hit
AGCGAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGAC870.12746692453078987No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.12600178746721757No Hit
AGCGAGGGGAGCCTTCTGTGGGCCTCAATGGAAAACCTGTGATTTCAACTTGGTGAGACAGCAG820.12014123921292837No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.11721096508578378No Hit
AGCGAGGGGGGAAAGGGAGACTGAGGAGCCCTGCGCGCACAAGTGATCAAGCCAGCGCTCCGCC790.11574582802221148No Hit
AGCGAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG790.11574582802221148No Hit
AGCGAGGGGAACTCACCCTGGACCTCCAAGACATGGAGGACAATGAGGGCTTTAGCCACATCCT760.11135041683149459No Hit
AGCGAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT750.10988527976792228No Hit
AGCGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.1069550056407777No Hit
AGCGAGGGGACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTA710.1040247315136331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCA150.00461142658.1798150
TAATACC150.00461142658.1798155
TTGTGGA150.00461142658.1798153
CATGCAT150.00461142658.1798158
ACTTCGG150.00461142658.1798151
ATTAAGC150.00461142658.1798151
AAGAACT150.00461142658.1798153
CCTAATA150.00461142658.1798153
CTACGCA150.00461142658.1798157
TTGACCC150.00461142658.1798157
CTTGACC150.00461142658.1798156
CGGTCTA150.00461142658.1798153
GCCAGTT150.00461142658.1798152
AACTCAA150.00461142658.1798156
CTTTAGC150.00461142658.1798150
TCAAGTA150.00461142658.1798158
CTGGCAT150.00461142658.1798151
AAAGAAC150.00461142658.1798152
TTGATAT258.426363E-658.17980656
GGTCAAG201.9665383E-458.17980657
Produced by FastQC (version 0.11.5)