FastQCFastQC Report
Fri 17 Jun 2016
SRR1526519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66060
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4310.652437178322737No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.646382076899788No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.37390251286709053No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2440.3693611867998789No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2320.3511958825310324No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG2160.32697547683923706No Hit
AGCGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.2149561005146836No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1150.174084165909779No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1090.16500151377535574No Hit
AGCGAGGGGCATTCAACCCAGGACTGAAAGAGGCTCTCTGTCCCTTTTGACAGAAAGGTCAAGT1090.16500151377535574No Hit
AGCGAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1070.16197396306388132No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1070.16197396306388132No Hit
AGCGAGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1050.15894641235240692No Hit
AGCGAGGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCACATTATTGACAGTCTTGCATTTTG1040.15743263699666968No Hit
AGCGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA970.14683620950650922No Hit
AGCGAGGGGGGGATCCTGGCTGCACAACTGTTAGATCTGGTTGTGAGGCATCCGGTTGATATCT910.137753557372086No Hit
AGCGAGGGGATGCGAGGGGTTAATTTTGCCCAGAGCTTTGCTATCTTTATTTCCATAGTGTTGG910.137753557372086No Hit
AGCGAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG900.13623978201634876No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG900.13623978201634876No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.11656070239176505No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC730.11050560096881623No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1059642749016046No Hit
AGCGAGGGGAGACTAAGGATTTTGGATCCCAGGAGGCCTCAAAAGAAGAAAAAGAAGATGTGAT680.1029367241901302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGG201.9601967E-458.2149434
CGCGCAC201.9601967E-458.2149434
GTCTATG150.004599739358.2149434
GTAGGAT201.9601967E-458.2149433
CCCGTCT150.004599739358.2149436
TAGGATT150.004599739358.2149434
AAGGCTA303.613386E-758.2149436
CACATTA150.0046136558.170639
TATGGTT150.0046136558.170637
GACAAAG201.967604E-458.170638
CTCTTGT150.0046136558.170639
GTGAGCA150.0046136558.170638
AGGCTAG201.967604E-458.170637
ACCCGTC150.004627592458.1263335
CCAACAC150.004627592458.1263328
AAAGGCT201.9750337E-458.1263335
GTACCTT201.9824857E-458.0821344
ACAGTCT150.004641566458.0821349
CGAACGT150.004641566458.0821349
GCAAGCC201.9824857E-458.0821349
Produced by FastQC (version 0.11.5)