SRR1526518_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526518_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60607
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	515	0.8497368290791494	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	326	0.5378916626792285	No Hit
AGCGAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	301	0.4966423020443183	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	281	0.4636428135363902	No Hit
AGCGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	281	0.4636428135363902	No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.4438431204316333	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	249	0.41084363192370515	No Hit
AGCGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	208	0.3431946804824525	No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	132	0.21779662415232562	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC	117	0.19304700777137954	No Hit
AGCGAGAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	111	0.1831471612190011	No Hit
AGCGAGGGGGATGCGTGCTAGGACCACCGTGGCAGCTTGAGGGCGGCCTAGAGGCC	108	0.1781972379428119	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	104	0.17159734024122625	No Hit
AGCGAGGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCACATTATTGACAGTCTT	104	0.17159734024122625	No Hit
AGCGAGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAG	101	0.16664741696503704	No Hit
AGCGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	99	0.16334746811424425	No Hit
AGCGAGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT	97	0.1600475192634514	No Hit
AGCGAGGGGAGACTAAGGATTTTGGATCCCAGGAGGCCTCAAAAGAAGAAAAAGAA	86	0.14189780058409096	No Hit
AGCGAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC	85	0.14024782615869455	No Hit
AGCGAGGGGGGGATCCTGGCTGCACAACTGTTAGATCTGGTTGTGAGGCATCCGGT	83	0.13694787730790173	No Hit
AGCGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81	0.1336479284571089	No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.1286980051809197	No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGGGCGCCTGCCCAGCAAGCGC	78	0.1286980051809197	No Hit
AGCGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.12209810747933407	No Hit
AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.12209810747933407	No Hit
AGCGAGGGGCATTCAACCCAGGACTGAAAGAGGCTCTCTGTCCCTTTTGACAGAAA	72	0.11879815862854126	No Hit
AGCGAGGGGTGATAAATTCATAGAAAATCAATTGAGAACCTTTATTGATAAAAATT	72	0.11879815862854126	No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	71	0.11714818420314485	No Hit
AGCGAGGGGGTTAATATGGGTAAATCTGGTTGTTTAAGGCTACCAAATCTTTGCAG	71	0.11714818420314485	No Hit
AGCGAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA	70	0.11549820977774844	No Hit
AGCGAGGGGAGCCTTCTGTGGGCCTCAATGGAAAACCTGTGATTTCAACTTGGTGA	69	0.11384823535235204	No Hit
AGCGAGGGGGGTTTAATATTTCAACAGACCGACGTGACAAATTGGAACGAGCAACC	68	0.11219826092695563	No Hit
AGCGAGGGGAACTCACCCTGGACCTCCAAGACATGGAGGACAATGAGGGCTTTAGC	68	0.11219826092695563	No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	67	0.11054828650155922	No Hit
AGCGAGGGGAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGGGGCC	66	0.10889831207616281	No Hit
AGCGAGGGGGGAAAGGGAGACTGAGGAGCCCTGCGCGCACAAGTGATCAAGCCAGC	65	0.10724833765076641	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTACG	15	0.00793873	50.707317	37
TTGAGGG	20	3.8736928E-4	50.707317	37
GAAGGAC	15	0.00793873	50.707317	37
TCAGTAC	15	0.0079651885	50.664703	36
CGCACAA	20	3.8898227E-4	50.664703	36
AGCTTGA	20	3.9060062E-4	50.622166	34
TGACCTG	15	0.007991713	50.622166	35
TAGCTGT	15	0.007991713	50.622166	34
ACTCAGT	15	0.007991713	50.622166	34
AAGACGC	20	3.9060062E-4	50.622166	34
CGGGAAG	20	3.9060062E-4	50.622166	34
TAGGGCG	25	1.917933E-5	50.622166	34
AGCTGAC	15	0.007991713	50.622166	35
CGCGCAC	20	3.9060062E-4	50.622166	34
TAAAAGG	40	2.4447218E-9	50.368423	31
CGAAGTG	15	0.008152238	50.368423	32
AGCATAT	60	0.0	50.368423	31
GGCTACT	15	0.008152238	50.368423	32
GTAAAGT	15	0.008152238	50.368423	33
CCGAAGT	15	0.008152238	50.368423	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content