FastQCFastQC Report
Fri 17 Jun 2016
SRR1526517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109370
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG14341.3111456523726799No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.8265520709518149No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6210.5677973850233153No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA4870.44527749839992686No Hit
TTGGACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4280.39133217518515134No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.324586266800768No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.3181859742159642No Hit
TTGGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2720.24869708329523635No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.20023772515314983No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2060.18835146749565693No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.18469415744719758No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1870.170979244765475No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.16092164213221175No Hit
TTGGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1750.1600073146200969No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.15452134954740787No Hit
TTGGACGGGAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGTGTTGGATGAGTT1550.14172076437780012No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.1389777818414556No Hit
TTGGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.12434854164761817No Hit
TTGGACGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT1320.12069123159915883No Hit
TTGGACGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1310.11977690408704399No Hit
TTGGACGGGATTGACCAGATAGCAGCCACTATGTATTTAGGGTTTGGTTTGGTTTTTGAGCCAA1280.11703392155069947No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.11611959403858463No Hit
TTGGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.11429093901435496No Hit
TTGGACGGGGCAAAAAATATTGTCGTTGGCTTCGCAAGAATGAATGGGAGGACCGTTGGAATTG1250.11429093901435496No Hit
TTGGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1220.11154795647801041No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1200.10971930145378074No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1190.1088049739416659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGC201.9643389E-458.22467457
TTGAGCG201.9643389E-458.22467454
CCACGTC150.0046048158.22467457
TCCCACG201.9643389E-458.22467456
GCCACGT150.0046048158.22467456
CATTTCG150.0046048158.22467456
ATTTCGC150.0046048158.22467457
TCCGAAC150.004613214658.1978852
TATCTCA201.9688174E-458.1978848
ATTAAGC650.058.17111651
GACCGTT150.00462163158.17111251
CCAACCA150.004638497358.1176540
AGTAGGC150.004638497358.1176539
TTAGGGT258.560848E-658.06427837
TAGAGAG201.9913318E-458.06427835
GCAAACT303.701516E-758.06427837
GATTTAT201.9913318E-458.06427835
GTTGCCG303.701516E-758.06427837
ATCCGAC150.004672367658.0110127
TCGCGAC150.004672367658.0110134
Produced by FastQC (version 0.11.5)