FastQCFastQC Report
Fri 17 Jun 2016
SRR1526516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106213
Sequences flagged as poor quality0
Sequence length64
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG12041.1335712200954684No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.7023622343780893No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4910.46227862879308557No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA3870.3643621778878292No Hit
TTGGACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC3350.315403952435201No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.2833928050238671No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.26927023998945515No Hit
TTGGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2490.2344345795712389No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.17135378908419874No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.15158219803602196No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.14969918936476703No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1540.14499166768662972No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1500.14122565034411985No Hit
TTGGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1410.13275211132347264No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1410.13275211132347264No Hit
TTGGACGGGGCAAAAAATATTGTCGTTGGCTTCGCAAGAATGAATGGGAGGACCGTTGGAATTG1320.12427857230282546No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.11768804195343319No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.11392202461092332No Hit
TTGGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.10638998992590361No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.10356547691902122No Hit
TTGGACGGGAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGTGTTGGATGAGTT1100.10356547691902122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA150.004548319658.40505633
GCAATTC201.9387889E-458.37720537
CAATTCC201.952551E-458.29380438
CTTCGTT150.00461748558.18298356
CGTGGCC150.00461748558.18298344
GATCGAA150.00461748558.18298349
TTAGACT150.00461748558.18298348
TACCTTT150.00461748558.18298344
TTAGTTC150.00461748558.18298358
GCAAGGC501.8189894E-1258.1553555
ACGTGCT150.004626185658.15534631
TTGGTAG150.004626185658.15534652
GTACTGG150.004626185658.15534631
GGTAGCC150.004626185658.15534654
TGGTAGC150.004626185658.15534653
GTAGCCA201.9756604E-458.15534255
AACGTCT150.004634898658.1277351
CCCGTCG150.004634898658.1277342
ACCCGTC150.004634898658.1277341
CTACTTA150.004643623358.10014343
Produced by FastQC (version 0.11.5)