FastQCFastQC Report
Fri 17 Jun 2016
SRR1526515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95624
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA10631.111645611980256No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9110.95268970133021No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6160.6441897431607128No Hit
TTGGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT4200.4392202794277587No Hit
TTGGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.36601689952313227No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.3461474106918765No Hit
TTGGACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG3210.3356897849912156No Hit
TTGGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2430.2541203045260604No Hit
TTGGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.24784572910566383No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2130.22274742742407766No Hit
TTGGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.19346607546222708No Hit
TTGGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.17777963691123566No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1680.17568811177110347No Hit
TTGGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.15268133522964947No Hit
TTGGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1250.13072032125826152No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.12549150840793108No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.12444574583786497No Hit
TTGGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.12235422069773279No Hit
TTGGACGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC1130.11817117041746843No Hit
TTGGACGGGAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGTGTTG1050.10980506985693968No Hit
TTGGACGGGGCAAAAAATATTGTCGTTGGCTTCGCAAGAATGAATGGGAGGACCGT1030.1077135447168075No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1000.10457625700660922No Hit
TTGGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC960.10039320672634484No Hit
TTGGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA960.10039320672634484No Hit
TTGGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.10039320672634484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCCT203.930619E-450.58842537
CCTTACC251.9341685E-550.5884237
ATATAAT505.456968E-1250.5884237
TGGGACC150.00802721150.5884237
TTAGGCA150.00807792150.50795435
AGAACTA150.00807792150.50795436
CACAGTA203.961649E-450.50795435
CAAATAT451.2187229E-1050.50795434
GCCTTAC251.952493E-550.5079536
AATATAA505.456968E-1250.5079536
GTACCAC204.003324E-450.4010631
TATGCGG150.00814590450.4010632
TGTTAGG150.00814590450.4010633
CACAAAT451.2369128E-1050.4010632
TGTGTTA150.00814590450.4010631
TAATTAT550.050.16219340
TAGGGCA204.098368E-450.1621940
TGAATTT150.00830042350.1621940
TGTTTCA150.00833505450.10941749
GCCTTCG150.00833505450.10941750
Produced by FastQC (version 0.11.5)