FastQCFastQC Report
Fri 17 Jun 2016
SRR1526514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107954
Sequences flagged as poor quality0
Sequence length64
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11861.0986160772180742No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.5567186023676751No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.33903329195768567No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2930.2714118976601145No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG2750.2547381292031791No Hit
TCACTTGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA2560.23713804027641402No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC2550.23621171980658429Illumina Single End Adapter 2 (96% over 31bp)
TCACTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2250.20842210571169204No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT2220.2056431443022028No Hit
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.18989569631509717No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1930.17877985067714025No Hit
TCACTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1770.16395872315986437No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1590.14728495470292902No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1560.1445059932934398No Hit
TCACTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1390.12875854530633418No Hit
TCACTTGGGGAGTCTTAAGGACTGCTCTGTGTAACACTGTGACTGCCCTGTGTGCTTAGACACG1390.12875854530633418No Hit
TCACTTGGGAAGCAGTGGTATCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAG1350.12505326342701523No Hit
TCACTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA1310.12134798154769624Illumina Single End Adapter 2 (97% over 34bp)
TCACTTGGGGTCTCCATCTGTCTGTCTTAGCTCCCTGTCTTAACTCTATATGCTGTCTCCATAG1300.1204216610778665No Hit
TCACTTGGGTGCTGGTTGATCTAGGACATTCCTCAGCCTGTGCACACAAACGCCTGTTCTTTTT1290.11949534060803677No Hit
TCACTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.11301109731922857No Hit
TCACTTGGGAGTTCCTTCATTCTACCTTGAAGGAATCAGCACACAGTTAGAAGCAGAATTCTTT1120.10374789262093113No Hit
TCACTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.10096893121144192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAT201.9732225E-458.17093758
CTACTAG258.467423E-658.17093755
TGGACGT258.467423E-658.17093754
CTATACC303.654368E-758.17093758
GTGTGAA150.004621529558.17093755
GTAGACC150.004621529558.17093758
AACGAAT150.004621529558.17093753
GCCTACC150.004621529558.17093755
ACGTCTT258.467423E-658.17093757
TGCTATA303.654368E-758.17093756
CCCGGTC150.004621529558.17093755
TAAGCGG201.9732225E-458.17093757
TTACAGG150.004621529558.17093756
GATAAAC150.004630062758.14385652
CGACCGT150.004630062758.14385650
GCGCCCA150.004630062758.14385652
AAGAACG150.004630062758.14385650
TATGCTG150.004630062758.14385649
TGACGCC150.004630062758.14385651
CCCAAGT258.4908515E-658.14385652
Produced by FastQC (version 0.11.5)