FastQCFastQC Report
Fri 17 Jun 2016
SRR1526513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106440
Sequences flagged as poor quality0
Sequence length64
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.8455467869222096No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.40680195415257425No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2870.26963547538519356No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.262119503945885No Hit
TCACTTGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA2340.21984216459977451No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC2190.20574971815107101Illumina Single End Adapter 2 (96% over 31bp)
TCACTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT2050.19259676813228108No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG2020.1897782788425404No Hit
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.16629086809470123No Hit
TCACTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1600.1503194287861706No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1530.14374295377677565No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1450.1362269823374671No Hit
TCACTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1330.12495302517850432No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1290.12119503945885006No Hit
TCACTTGGGTGCTGGTTGATCTAGGACATTCCTCAGCCTGTGCACACAAACGCCTGTTCTTTTT1150.10804208944006012No Hit
TCACTTGGGTTGCCTAGGTTTGGGTAGTTAGAAATTGTTTGTGGCCCACAGGCCTTGCTATACC1140.10710259301014656No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGTGGTATCAA1130.10616309658023299No Hit
TCACTTGGGAAGCAGTGGTATCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAG1090.10240511086057874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG150.004593507458.25961334
AAAGCGA150.004593507458.25961333
GTTGATG150.004610805358.2043637
AAGTAGT150.004610805358.2043654
CGAACTG150.004610805358.2043655
ATTAATC150.004610805358.2043657
CGCCTGA150.004610805358.2043657
AAGTTCG150.004610805358.2043657
AACTGAC150.004610805358.2043657
TCAAGGA150.004610805358.2043637
TCGAACT150.004610805358.2043654
CGCCCCT150.004610805358.2043658
AGCGCAA201.9720866E-458.1767852
GCAGGTG201.9720866E-458.1767839
CCTACCG150.004619472758.17677739
CCGACGA150.004619472758.17677731
CGCTTGT150.004619472758.17677731
AACACTC150.004619472758.17677739
CGCGATT201.9767151E-458.1492230
ACTCTAT150.004636843758.12168543
Produced by FastQC (version 0.11.5)