##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526513_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 106440 Sequences flagged as poor quality 0 Sequence length 64 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.35561818865088 39.0 38.0 40.0 35.0 40.0 2 38.119212701991735 39.0 38.0 40.0 35.0 40.0 3 38.00813603908305 39.0 38.0 40.0 35.0 40.0 4 38.08568207440812 39.0 38.0 40.0 35.0 40.0 5 38.17409808342728 39.0 38.0 40.0 35.0 40.0 6 38.257741450582486 39.0 38.0 40.0 35.0 40.0 7 38.0511086057873 39.0 38.0 40.0 35.0 40.0 8 37.99735062006764 39.0 38.0 40.0 35.0 40.0 9 38.0880308154829 39.0 38.0 40.0 35.0 40.0 10 37.48182074408117 39.0 38.0 40.0 33.0 40.0 11 37.67462420142803 39.0 38.0 40.0 34.0 40.0 12 37.54920142803457 39.0 38.0 40.0 33.0 40.0 13 37.53166102968809 39.0 38.0 40.0 33.0 40.0 14 37.485569334836526 39.0 38.0 40.0 33.0 40.0 15 37.212795941375425 39.0 38.0 40.0 33.0 40.0 16 36.96255167230365 39.0 37.0 40.0 32.0 40.0 17 36.757703870725294 39.0 37.0 40.0 32.0 40.0 18 37.01048478015784 39.0 37.0 40.0 32.0 40.0 19 36.762561067267946 39.0 36.0 40.0 32.0 40.0 20 36.878523111612175 39.0 36.0 40.0 32.0 40.0 21 36.7977546035325 39.0 37.0 40.0 32.0 40.0 22 36.71315295001879 39.0 36.0 40.0 31.0 40.0 23 36.55738444193912 39.0 36.0 40.0 31.0 40.0 24 36.48555993987223 39.0 36.0 40.0 31.0 40.0 25 36.39211762495302 39.0 36.0 40.0 31.0 40.0 26 36.38823750469748 39.0 36.0 40.0 31.0 40.0 27 35.898327696354755 39.0 36.0 40.0 29.0 40.0 28 35.729622322435176 39.0 36.0 40.0 29.0 40.0 29 35.6402574220218 39.0 36.0 40.0 28.0 40.0 30 35.49096204434423 39.0 35.0 40.0 28.0 40.0 31 35.13248778654641 39.0 35.0 40.0 26.0 40.0 32 35.08709131905299 39.0 35.0 40.0 26.0 40.0 33 35.128344607290494 39.0 35.0 40.0 26.0 40.0 34 34.54633596392334 39.0 35.0 40.0 23.0 40.0 35 34.555026305900036 39.0 35.0 40.0 24.0 40.0 36 34.13707252912439 38.0 35.0 40.0 20.0 40.0 37 33.9945509207065 38.0 34.0 40.0 20.0 40.0 38 34.00127771514468 38.0 34.0 40.0 21.0 40.0 39 33.555345734686206 38.0 33.0 40.0 15.0 40.0 40 33.710165351371664 38.0 33.0 40.0 19.0 40.0 41 33.5402292371289 38.0 33.0 40.0 16.0 40.0 42 33.29342352499061 38.0 33.0 40.0 11.0 40.0 43 33.25287485907553 38.0 33.0 40.0 14.0 40.0 44 33.09301954152574 38.0 33.0 39.0 11.0 40.0 45 32.94098083427283 38.0 33.0 39.0 9.0 40.0 46 32.73186771890267 38.0 33.0 39.0 4.0 40.0 47 32.567888012025556 38.0 32.0 39.0 4.0 40.0 48 32.42403231867719 38.0 32.0 39.0 4.0 40.0 49 32.16239195791056 37.0 32.0 39.0 4.0 40.0 50 31.730561818865088 37.0 31.0 39.0 4.0 40.0 51 31.378344607290494 37.0 31.0 39.0 4.0 40.0 52 31.268855693348364 37.0 31.0 39.0 4.0 40.0 53 30.972886133032695 36.0 30.0 39.0 4.0 40.0 54 30.66389515219842 36.0 29.0 39.0 4.0 40.0 55 30.375056369785796 36.0 29.0 39.0 4.0 40.0 56 29.714740698985345 36.0 28.0 39.0 4.0 40.0 57 29.336790680195417 35.0 27.0 39.0 4.0 40.0 58 29.08085306275836 35.0 26.0 39.0 4.0 40.0 59 28.726456219466368 35.0 24.0 39.0 4.0 40.0 60 28.394184517098836 35.0 23.0 39.0 4.0 40.0 61 28.021335963923338 35.0 18.0 39.0 4.0 40.0 62 27.762664411875235 35.0 15.0 39.0 4.0 40.0 63 27.439524614806462 35.0 4.0 39.0 4.0 40.0 64 27.299201428034575 34.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.36741841817948284 2 2 -0.35704917655105106 2 3 -0.6345830534660593 2 4 0.034749384926492155 2 5 -0.594151305420084 2 6 -0.35063841807556884 2 7 -0.12041451501598033 2 8 0.2959026695371634 2 9 -0.05573824101796987 2 10 -0.5313799007642217 2 11 0.03227364978548053 2 12 0.3416280634617337 2 13 0.3021487585056164 2 14 0.1310812602134277 2 15 0.8756644410648562 2 16 0.34647296849114895 2 17 0.8167440572516185 2 18 0.31053679185497174 2 19 0.5005151125540408 2 20 0.6954372584405064 2 21 -0.2024102962799148 2 22 -0.42771049435625486 2 23 -0.23938371597471786 2 24 -0.11750312486599768 2 25 -0.17958392121686728 2 26 0.4088798063970671 2 27 0.8702596362558879 2 28 0.48600026296603005 2 29 0.49542453209135573 2 30 0.47071535380224105 2 31 0.9178494967785653 2 32 1.0299413292742798 2 33 0.5146726148153675 2 34 0.8051378502990829 2 35 1.1067521249556336 2 36 -0.2896466743112214 2 37 -0.6749803675957722 2 38 -0.07530219039941954 2 39 -3.8318488699724362 2 40 -0.5239153000212156 2 41 -2.5471443621202745 2 42 -2.47236786653599 2 43 -2.847551792362008 2 44 -2.62192766923679 2 45 -2.3375158269278167 2 46 -1.4461609010695362 2 47 -1.5766087198262788 2 48 -1.4337853994154486 2 49 -1.5688017461866117 2 50 -1.0550434205000379 2 51 -0.9732918479980057 2 52 -0.9487595803330144 2 53 -0.8082047548040379 2 54 -1.4715061879144429 2 55 -1.5104170545974505 2 56 -1.1305942315613393 2 57 -0.6956959881268325 2 58 -0.7463676555409826 2 59 -1.7437327879526663 2 60 -1.1875146590494374 2 61 -1.540368619386168 2 62 -1.0953488877146285 2 63 -1.2949297939946476 2 64 -1.9081577270440064 3 1 0.09426382481117201 3 2 -0.3810231708623064 3 3 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-4.132070280781349 3 44 -3.863211700118544 3 45 -4.035037159719366 3 46 1.323688753542406 3 47 1.407381528040439 3 48 1.6617042389429457 3 49 1.691498944589494 3 50 2.2878253320395814 3 51 2.0029779610227187 3 52 2.128363540349632 3 53 2.0596538392634116 3 54 1.793426766169265 3 55 2.0288759157398104 3 56 0.8519331963135102 3 57 0.8721219720519535 3 58 0.5576550994366691 3 59 1.0002737134695217 3 60 0.07611801059704959 3 61 0.8888065126739697 3 62 1.0538587514564384 3 63 0.9593570812593164 3 64 1.3758731547113783 4 1 -0.2251107258717866 4 2 0.050141458900455405 4 3 -0.275051280890807 4 4 0.17513400031110393 4 5 0.03695237350967773 4 6 -0.03349794984814025 4 7 -0.35645130431363725 4 8 0.14105317120940697 4 9 -0.2523101473724836 4 10 -0.39233307802174266 4 11 -0.34113772145197885 4 12 0.24806619055203782 4 13 0.29512534713438043 4 14 -0.05963780333172508 4 15 0.01111594608157418 4 16 0.3882789885580422 4 17 0.3486002445425882 4 18 -0.2859515024594117 4 19 0.13563216941022915 4 20 0.13013625509602633 4 21 -0.2736477544738918 4 22 -0.24401484218234515 4 23 -0.3551027795198678 4 24 -0.3234395797154974 4 25 -0.31369763359145253 4 26 0.524598869942217 4 27 0.45111247906525165 4 28 1.068943406778743 4 29 0.6506920906197848 4 30 0.9537421096550887 4 31 -0.0099097005458475 4 32 -0.5213964633678572 4 33 -0.059324040703025105 4 34 0.02085691384423427 4 35 -4.0093849987901855 4 36 -0.3026901525720902 4 37 -0.018375016425203228 4 38 -0.4321583776903921 4 39 0.002599290562677936 4 40 0.22324189061423994 4 41 1.1486750358730333 4 42 1.295274273932236 4 43 1.4194214517851478 4 44 1.8167847053451496 4 45 1.5783704606975988 4 46 1.0455615069572204 4 47 0.820214022648635 4 48 1.2121176106179945 4 49 0.49775343775987224 4 50 1.2629331681287255 4 51 0.7767917640421267 4 52 0.769718680536549 4 53 0.8534173187745573 4 54 0.5330924742929142 4 55 1.084231774833988 4 56 0.3338706513818046 4 57 0.19050802525109134 4 58 -0.12997969567476275 4 59 0.632103332448672 4 60 0.07485992289036503 4 61 -0.4825090876135931 4 62 -0.8410010616276722 4 63 0.24629094179130817 4 64 -0.13014769359919143 5 1 -0.03778878854985379 5 2 0.4960104047694003 5 3 0.17580341996389137 5 4 0.40697160714871217 5 5 0.03837687493417974 5 6 0.0793937880435962 5 7 0.2357851629228307 5 8 0.422392202548437 5 9 0.2573052372429032 5 10 0.1885073778187092 5 11 -1.5021063824206422 5 12 -1.6062785387926937 5 13 -1.41427636226733 5 14 -1.3570024756963974 5 15 -0.9689410339754048 5 16 -19.022689672410618 5 17 -2.7756875047451643 5 18 -2.3016210181289267 5 19 -2.9932852095071496 5 20 -2.168652918693148 5 21 -2.1383201191462575 5 22 -2.2856815088490094 5 23 -2.4811711555882425 5 24 -1.731915363191277 5 25 -1.9233842432780577 5 26 0.9562228015661489 5 27 1.8339472369000092 5 28 1.32891491675025 5 29 1.891076706004398 5 30 1.6050953860083652 5 31 1.8383808977447487 5 32 1.4016804597090626 5 33 1.1233682669892815 5 34 2.3506289936163114 5 35 -2.231251095656283 5 36 1.484346884464948 5 37 1.2711548981047116 5 38 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-30.87663173301072 120 34 -30.325296932309612 120 35 -30.249769614174802 120 36 -29.80269015257209 120 37 -29.70106732411751 120 38 -29.70138914692116 120 39 -29.26663147866809 120 40 -29.3152196478473 120 41 -29.21670957951158 120 42 -29.002802649144684 120 43 -28.917117009753312 120 44 -28.76975375619331 120 45 -28.63316800084086 120 46 -28.36790003150432 120 47 -28.23747828504367 120 48 -28.085959312458925 120 49 -27.925323485317048 120 50 -27.515912985717428 120 51 -27.15590054365018 120 52 -27.10528131946345 120 53 -26.790813450456213 120 54 -26.51498444878401 120 55 -26.22346053285832 120 56 -25.521898579387425 120 57 -25.16526120551814 120 58 -24.93767200336707 120 59 -24.569819744474405 120 60 -24.213601615571175 120 61 -23.905586010690516 120 62 -23.591001061627672 120 63 -23.321016750516385 120 64 -23.082070770522268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 120.0 5 23.0 6 67.0 7 61.0 8 222.0 9 173.0 10 275.0 11 291.0 12 311.0 13 443.0 14 503.0 15 549.0 16 649.0 17 735.0 18 788.0 19 870.0 20 860.0 21 1125.0 22 1147.0 23 824.0 24 937.0 25 1094.0 26 1216.0 27 1474.0 28 1789.0 29 2100.0 30 2486.0 31 3110.0 32 3678.0 33 4625.0 34 5863.0 35 7139.0 36 9432.0 37 13275.0 38 18866.0 39 19218.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.57909769067437 0.21702775324602117 0.11743926041451361 0.08643529566508201 8 99.24419752390085 0.41550335128834237 0.23595326057324423 0.10434586423757015 9 96.75497933107854 1.8573844419391206 0.5232995114618565 0.864336715520481 10 39.293498684705 39.88726042841037 8.201803833145433 12.617437053739195 11 26.164975573092825 35.5815482901165 20.974257797820368 17.279218338970313 12 42.593949642991355 17.53382187147689 20.627583615182264 19.244644870349493 13 22.467117624953026 16.7314919203307 27.71138669673055 33.09000375798572 14 24.76042841037204 28.6978579481398 25.203870725291246 21.33784291619692 15 35.130712807974426 20.481474515704345 24.262742147827723 20.12507052849351 16 23.715418734024958 23.291610284167792 30.518906931288527 22.47406405051872 17 34.33736073471846 21.626580849141824 20.664897621198435 23.371160794941282 18 30.96674182638106 22.87955655768508 23.130402104472005 23.023299511461857 19 23.72322435174746 22.715144682450205 30.90567455843668 22.65595640736565 20 22.01827102013196 30.133085208368577 23.0652831820147 24.783360589484765 21 22.77057497181511 21.731491920330704 32.01615933859451 23.481773769259675 22 23.61424276587749 23.458286358511838 23.41694851559564 29.510522360015035 23 25.426531379180762 30.621007140172868 21.470311912814733 22.48214956783164 24 23.11876955455545 32.15825918653049 23.775520750143283 20.947450508770775 25 25.022326254735518 20.845671526739803 25.65028154582287 28.481720672701805 26 28.82354599532012 21.3648708334508 26.41970435942977 23.39187881179931 27 22.925708690699228 20.395981810821244 26.607793479386537 30.07051601909299 28 23.355226015280966 28.81459595769506 26.329557002521735 21.50062102450224 29 29.597328064728572 22.86009972716154 27.12766958321573 20.414902624894157 30 20.840943755060536 28.471764550812512 30.701225827103773 19.98606586702318 31 26.229940480675058 23.555337904015673 30.287990657726212 19.926730957583064 32 21.710204388903527 22.457371927579327 38.19776414348628 17.634659540030864 33 24.211121050896935 23.692264230895994 33.90084278920853 18.195771928998543 34 20.330463157696187 25.27232088123509 35.27392415568738 19.123291805381342 35 18.18447278687289 31.10434995572804 35.50140351538215 15.20977374201692 36 21.882896473694135 26.38789364510654 34.64454082594758 17.084669055251744 37 19.34854157432643 25.665085487415006 38.10013296994502 16.886239968313546 38 21.105129679736777 25.84826857988668 36.92879163563341 16.11781010474314 39 19.45366665720799 24.52919421507146 37.00235521125961 19.01478391646094 40 19.03657179501688 24.58929817612554 40.01584337690262 16.35828665195496 41 17.00756646602638 26.426967564201746 39.55695605411627 17.008509915655605 42 18.52890626475532 24.66239824730391 36.83778117740382 19.97091431053695 43 18.344301454967756 25.823081208161412 37.584621340156545 18.247995996714284 44 17.396064366948234 23.983766693407578 39.124156481525176 19.496012458119015 45 17.98432779456193 25.54475075528701 39.36367069486405 17.10725075528701 46 17.415613607600914 26.07714247936382 38.35685007838915 18.150393834646113 47 18.909015356137733 25.734280263679803 38.20996165687626 17.146742723306197 48 21.255723929566162 26.21441722135675 36.796487749610534 15.733371099466552 49 20.654288574504655 26.75707836730068 36.74423436526075 15.84439869293391 50 16.735998184774797 28.836554097492723 37.5118178724048 16.915629845327686 51 16.96567752175269 28.853366588253078 37.906829540194046 16.274126349800184 52 18.63477874684633 27.140953802832872 36.99742036681817 17.22684708350263 53 16.787017258652956 27.619515699136123 36.94637152416779 18.647095518043137 54 17.74289575399038 29.175841310942474 35.88553824054736 17.195724694519786 55 18.731626161510174 27.829925606632074 35.01781848774447 18.42062974411328 56 18.37392685601906 28.90208388487576 34.753791460232215 17.97019779887296 57 19.09263653466189 28.4498813564129 35.426691498312515 17.03079061061269 58 18.96509317945322 29.453221076530127 33.53325134802762 18.048434395989027 59 18.261881337974874 30.077001664900866 34.84467231723929 16.81644467988497 60 18.885103161082718 30.616127200454287 33.36267272383115 17.13609691463184 61 19.54086022522054 28.63855979879542 34.568800052948575 17.251779923035464 62 17.627811087363582 29.148304363450663 32.845983620510296 20.377900928675455 63 18.737469264233024 31.66635142803102 30.72347266881029 18.872706638925667 64 19.626053204582032 30.697718451197574 31.519454700369216 18.156773643851178 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 2.0 4 4.5 5 10.0 6 13.0 7 76.0 8 139.0 9 163.0 10 188.5 11 190.0 12 243.0 13 456.5 14 617.0 15 877.5 16 1286.0 17 1434.0 18 1679.0 19 1924.0 20 2144.0 21 2665.0 22 2966.0 23 3257.0 24 3642.5 25 3737.0 26 3740.5 27 3243.5 28 2743.0 29 2956.0 30 2811.5 31 2454.0 32 2349.0 33 2244.0 34 2253.5 35 2306.0 36 2349.0 37 2349.0 38 2271.0 39 2193.0 40 2321.0 41 2482.5 42 2516.0 43 2680.0 44 2844.0 45 2913.5 46 3024.5 47 3066.0 48 3346.0 49 3624.5 50 3623.0 51 3828.0 52 4549.5 53 5066.0 54 5408.0 55 5225.0 56 4700.0 57 4643.0 58 4586.0 59 4402.5 60 3952.0 61 3685.0 62 3193.5 63 2512.5 64 2323.0 65 2199.0 66 1639.0 67 1203.0 68 1044.0 69 885.0 70 766.5 71 607.0 72 566.0 73 447.0 74 256.5 75 185.0 76 148.5 77 103.0 78 94.0 79 71.0 80 40.0 81 32.0 82 23.0 83 14.0 84 9.0 85 3.0 86 2.0 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0018789928598271326 8 0.05918827508455468 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.09394964299135664 16 0.02254791431792559 17 0.15783540022547915 18 0.0 19 0.0 20 0.03945885005636979 21 0.0 22 0.0 23 0.0 24 0.006576475009394964 25 0.05918827508455468 26 0.025366403607666288 27 0.2095077038707253 28 0.15407741450582488 29 0.14092446448703494 30 0.21232619316046597 31 0.24051108605787297 32 0.1615933859451334 33 0.23017662532882374 34 0.3823750469748215 35 0.2611800075159714 36 0.3570086433671552 37 0.37673806839534013 38 0.34855317549793313 39 0.673618940248027 40 0.37767756482525366 41 0.4190154077414506 42 0.5129650507328072 43 0.49511461856444944 44 0.4556557685080797 45 0.4885381435550545 46 0.52423900789177 47 0.5204810221721158 48 0.4932356257046223 49 0.5204810221721158 50 0.6275836151822622 51 0.5552423900789176 52 0.574032318677189 53 0.5993987222848554 54 0.5853062758361518 55 0.6116121758737316 56 0.6350995866215708 57 0.6210071401728673 58 0.6858323938369034 59 0.6839534009770762 60 0.7328072153325818 61 0.6360390830514844 62 0.6557685080796692 63 0.6576475009394964 64 0.7609921082299886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 106440.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.69485155956407 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61180525635501 45.70274332957535 2 8.476121035362741 9.611048478015784 3 3.619129685480396 6.155580608793686 4 2.094587876578398 4.7500939496429915 5 1.3522023000695986 3.8331454340473505 6 0.8882113147515991 3.0214205186020293 7 0.6595300434162993 2.6174370537391956 8 0.4209061081098996 1.9090567455843668 9 0.3098796937659497 1.5811724915445322 >10 1.4615716037516986 13.831266441187523 >50 0.07125575845954993 2.8354002254791433 >100 0.033142213236999965 3.30608793686584 >500 0.0016571106618499983 0.8455467869222096 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 0.8455467869222096 No Hit TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 433 0.40680195415257425 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 287 0.26963547538519356 No Hit TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 279 0.262119503945885 No Hit TCACTTGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA 234 0.21984216459977451 No Hit TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC 219 0.20574971815107101 Illumina Single End Adapter 2 (96% over 31bp) TCACTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT 205 0.19259676813228108 No Hit TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG 202 0.1897782788425404 No Hit TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.16629086809470123 No Hit TCACTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 160 0.1503194287861706 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 153 0.14374295377677565 No Hit TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 145 0.1362269823374671 No Hit TCACTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 133 0.12495302517850432 No Hit TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 129 0.12119503945885006 No Hit TCACTTGGGTGCTGGTTGATCTAGGACATTCCTCAGCCTGTGCACACAAACGCCTGTTCTTTTT 115 0.10804208944006012 No Hit TCACTTGGGTTGCCTAGGTTTGGGTAGTTAGAAATTGTTTGTGGCCCACAGGCCTTGCTATACC 114 0.10710259301014656 No Hit TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGTGGTATCAA 113 0.10616309658023299 No Hit TCACTTGGGAAGCAGTGGTATCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAG 109 0.10240511086057874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0018789928598271326 0.0 0.0 0.0 0.0 9 0.0018789928598271326 0.0 0.0 0.0 0.0 10 0.042277339346110485 0.0 0.0 0.0 0.0 11 0.057309282224727545 0.0 0.0 0.0 0.0 12 0.06106726794438181 0.0 0.0 0.0 0.0 13 0.06200676437429538 0.0 0.0 0.0 0.0 14 0.08361518226230741 0.0 0.0 0.0 0.0 15 0.08737316798196167 0.0 0.0 0.0 0.0 16 0.09113115370161594 0.0 0.0 0.0 0.0 17 0.09864712514092447 0.0 0.0 0.0 0.0 18 0.1033446072904923 0.0 0.0 0.0 0.0 19 0.10710259301014656 0.0 0.0 0.0 0.0 20 0.1371664787673807 0.0 0.0 0.0 0.0 21 0.14937993235625704 0.0 0.0 0.0 0.0 22 0.1644118752348741 0.0 0.0 0.0 0.0 23 0.19071777527245395 0.0 0.0 0.0 0.0 24 0.22078166102968808 0.0 0.0 0.0 0.0 25 0.2264186396091695 0.0 0.0 0.0 9.394964299135663E-4 26 0.23393461104847801 0.0 0.0 0.0 9.394964299135663E-4 27 0.24051108605787297 0.0 0.0 0.0 9.394964299135663E-4 28 0.31942878617061254 0.0 0.0 0.0 9.394964299135663E-4 29 0.32130777903043967 0.0 0.0 0.0 9.394964299135663E-4 30 0.36170612551672304 0.0 0.0 0.0 9.394964299135663E-4 31 0.3664036076662909 0.0 0.0 0.0 9.394964299135663E-4 32 0.37298008267568583 0.0 0.0 0.0 9.394964299135663E-4 33 0.37861706125516725 0.0 0.0 0.0 9.394964299135663E-4 34 0.38989101841413004 0.0 0.0 0.0 9.394964299135663E-4 35 0.5157835400225479 0.0 0.0 0.0 9.394964299135663E-4 36 0.5251785043216836 0.0 0.0 0.0 9.394964299135663E-4 37 0.5355129650507328 0.0 0.0 0.0 9.394964299135663E-4 38 0.5505449079293498 0.0 0.0 0.0 9.394964299135663E-4 39 0.5674558436677941 0.0 0.0 0.0 9.394964299135663E-4 40 0.5834272829763247 0.0 0.0 0.0 9.394964299135663E-4 41 0.6003382187147689 0.0 0.0 0.0 9.394964299135663E-4 42 0.6097331830139046 0.0 0.0 0.0 9.394964299135663E-4 43 0.6228861330326945 0.0 0.0 0.0 9.394964299135663E-4 44 0.6407365652010523 0.0 0.0 0.0 9.394964299135663E-4 45 0.6501315295001879 0.0 0.0 0.0 9.394964299135663E-4 46 0.6576475009394964 0.0 0.0 0.0 9.394964299135663E-4 47 0.6726794438181135 0.0 0.0 0.0 9.394964299135663E-4 48 0.686771890266817 0.0 0.0 0.0 9.394964299135663E-4 49 0.7027433295753476 0.0 0.0 0.0 9.394964299135663E-4 50 0.7111987974445697 0.0 0.0 0.0 9.394964299135663E-4 51 0.7215332581736189 0.0 0.0 0.0 9.394964299135663E-4 52 0.7328072153325818 0.0 0.0 0.0 9.394964299135663E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACTCG 15 0.0045935074 58.259613 34 AAAGCGA 15 0.0045935074 58.259613 33 GTTGATG 15 0.0046108053 58.20436 37 AAGTAGT 15 0.0046108053 58.20436 54 CGAACTG 15 0.0046108053 58.20436 55 ATTAATC 15 0.0046108053 58.20436 57 CGCCTGA 15 0.0046108053 58.20436 57 AAGTTCG 15 0.0046108053 58.20436 57 AACTGAC 15 0.0046108053 58.20436 57 TCAAGGA 15 0.0046108053 58.20436 37 TCGAACT 15 0.0046108053 58.20436 54 CGCCCCT 15 0.0046108053 58.20436 58 AGCGCAA 20 1.9720866E-4 58.17678 52 GCAGGTG 20 1.9720866E-4 58.17678 39 CCTACCG 15 0.0046194727 58.176777 39 CCGACGA 15 0.0046194727 58.176777 31 CGCTTGT 15 0.0046194727 58.176777 31 AACACTC 15 0.0046194727 58.176777 39 CGCGATT 20 1.9767151E-4 58.14922 30 ACTCTAT 15 0.0046368437 58.121685 43 >>END_MODULE