SRR1526512_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95634
Sequences flagged as poor quality	0
Sequence length	56
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1237	1.2934730326034674	No Hit
TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	633	0.6618984879854445	No Hit
TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.3638873204090595	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	316	0.33042641738293915	No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	267	0.27918940962419225	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	249	0.2603676516719995	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGT	227	0.2373632808415417	No Hit
TCACTTGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA	210	0.21958717610891526	No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCA	196	0.20494803103498754	No Hit
TCACTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	190	0.19867411171758997	No Hit
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.19135453918062612	No Hit
TCACTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	164	0.17148712800886715	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	162	0.16939582156973462	Illumina Single End Adapter 2 (95% over 23bp)
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	158	0.16521320869146958	No Hit
TCACTTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC	132	0.13802622498274672	No Hit
TCACTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.1327979588849154	No Hit
TCACTTGGGGAGTCTTAAGGACTGCTCTGTGTAACACTGTGACTGCCCTGTGTGCT	116	0.12129577346968652	No Hit
TCACTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.12129577346968652	No Hit
TCACTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	114	0.11920446703055397	No Hit
TCACTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC	112	0.11711316059142146	No Hit
TCACTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.11188489449359014	No Hit
TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	107	0.11188489449359014	No Hit
TCACTTGGGAAGCAGTGGTATCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAA	103	0.10770228161532508	No Hit
TCACTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.10770228161532508	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	102	0.10665662839575883	No Hit
TCACTTGGGAGAAATTGCCGTTTTGTGATTCCTTTTGATTTTGTGTCTGTATAACA	100	0.10456532195662631	No Hit
TCACTTGGGTGCTGGTTGATCTAGGACATTCCTCAGCCTGTGCACACAAACGCCTG	100	0.10456532195662631	No Hit
TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	99	0.10351966873706005	No Hit
TCACTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98	0.10247401551749377	No Hit
TCACTTGGGCAGAGATCTTCAGCTTTGGATGATCCCTTTTCCATTCTGGATTTGCT	96	0.10038270907836125	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCCC	20	3.892779E-4	50.68747	37
TCGTGTT	20	3.892779E-4	50.68747	37
CAGTACG	25	1.9118548E-5	50.687466	37
TTTACCT	15	0.007998947	50.633457	35
CAGTTTA	15	0.007998947	50.633457	35
ATATGCT	25	1.9239666E-5	50.633457	34
AGAACGC	15	0.007998947	50.633457	34
AACCTCA	15	0.007998947	50.633457	35
GAACCTC	15	0.007998947	50.633457	34
TTGCATC	30	9.512478E-7	50.633457	36
CTTTACC	15	0.007998947	50.633457	34
AAAGGCT	25	1.9239666E-5	50.633457	35
AAGGCTA	25	1.9239666E-5	50.633457	36
CTCAGTA	25	1.9239666E-5	50.633457	35
GCGGTTT	15	0.00808357	50.498936	33
TAGAACG	15	0.00808357	50.498936	33
TGAACCT	15	0.00808357	50.498936	33
ACTTTAC	15	0.00808357	50.498936	33
CTGAACC	15	0.008117605	50.44533	32
AATCTTT	15	0.008117605	50.44533	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content