##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526512_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95634 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.55933036367819 39.0 39.0 40.0 36.0 40.0 2 38.26023171675346 39.0 38.0 40.0 35.0 40.0 3 38.0821987995901 39.0 38.0 40.0 35.0 40.0 4 38.34520149737541 39.0 39.0 40.0 35.0 40.0 5 38.1456072108246 39.0 38.0 40.0 35.0 40.0 6 38.46199050546877 39.0 38.0 40.0 36.0 40.0 7 38.134199134199136 39.0 38.0 40.0 35.0 40.0 8 38.42268440094527 39.0 38.0 40.0 36.0 40.0 9 38.35191459104502 39.0 38.0 40.0 36.0 40.0 10 37.63068573938139 39.0 38.0 40.0 33.0 40.0 11 37.872618524792436 39.0 38.0 40.0 35.0 40.0 12 37.824413911370435 39.0 38.0 40.0 35.0 40.0 13 37.84076792772445 39.0 38.0 40.0 35.0 40.0 14 37.816937490850535 39.0 38.0 40.0 34.0 40.0 15 37.77428529602442 39.0 38.0 40.0 34.0 40.0 16 37.617730095990964 39.0 38.0 40.0 34.0 40.0 17 37.62675408327582 39.0 38.0 40.0 33.0 40.0 18 37.51801660497313 39.0 38.0 40.0 33.0 40.0 19 37.2719430328126 39.0 38.0 40.0 33.0 40.0 20 37.43837965577096 39.0 38.0 40.0 33.0 40.0 21 37.245958550306376 39.0 38.0 40.0 33.0 40.0 22 37.16187757492105 39.0 38.0 40.0 33.0 40.0 23 36.92063492063492 39.0 37.0 40.0 33.0 40.0 24 36.85807348850827 39.0 37.0 40.0 32.0 40.0 25 36.780297802036934 39.0 37.0 40.0 32.0 40.0 26 36.322071648158605 39.0 36.0 40.0 31.0 40.0 27 36.26622330970157 39.0 36.0 40.0 31.0 40.0 28 35.94775916515047 39.0 36.0 40.0 30.0 40.0 29 35.89399167660037 39.0 36.0 40.0 30.0 40.0 30 35.5156638852291 38.0 35.0 40.0 29.0 40.0 31 34.9698642742121 38.0 35.0 40.0 27.0 40.0 32 34.51592529853399 38.0 34.0 40.0 25.0 40.0 33 34.576123554384424 38.0 35.0 40.0 25.0 40.0 34 34.70331681201247 38.0 35.0 40.0 25.0 40.0 35 34.44579333709768 38.0 34.0 40.0 24.0 40.0 36 33.99013949013949 38.0 34.0 40.0 19.0 40.0 37 33.53988121379426 38.0 34.0 40.0 13.0 40.0 38 33.618723466549554 38.0 33.0 40.0 17.0 40.0 39 33.54666750318924 38.0 33.0 40.0 17.0 40.0 40 33.1665830144091 38.0 33.0 40.0 10.0 40.0 41 32.91539619800489 38.0 33.0 39.0 4.0 40.0 42 32.8206391032478 38.0 33.0 39.0 4.0 40.0 43 32.64952841039798 38.0 32.0 39.0 4.0 40.0 44 32.219200284417674 38.0 32.0 39.0 4.0 40.0 45 32.14163372859025 37.0 32.0 39.0 4.0 40.0 46 31.699406068971285 37.0 31.0 39.0 4.0 40.0 47 31.495618713010018 37.0 31.0 39.0 4.0 40.0 48 31.099556643034905 36.0 30.0 39.0 4.0 40.0 49 31.01316477403434 36.0 30.0 39.0 4.0 40.0 50 30.616600790513836 36.0 30.0 39.0 4.0 40.0 51 30.027322918627267 36.0 29.0 39.0 4.0 40.0 52 29.801482736265346 36.0 29.0 39.0 4.0 40.0 53 29.459365915887656 36.0 28.0 39.0 4.0 40.0 54 29.082481125959387 35.0 27.0 39.0 4.0 40.0 55 28.612073112073112 35.0 24.0 39.0 4.0 40.0 56 17.099086099086097 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.034214391018061 1 2 -1.4188764434282106 1 3 -1.5099535692978208 1 4 -1.0009982426158643 1 5 -0.7667246318730037 1 6 -1.7686425114476378 1 7 -0.6306127770559513 1 8 -1.4642376038000435 1 9 -1.0859650668532836 1 10 -0.2858514751759884 1 11 -0.860660168087108 1 12 -1.1578668862333785 1 13 -0.8739910539735689 1 14 -0.7614390949876011 1 15 -0.9278336973877828 1 16 -1.8652814052857565 1 17 -1.6998858885099324 1 18 -1.2091864127687515 1 19 -1.4950299014807271 1 20 -1.0837867702584134 1 21 -0.8622698670574493 1 22 -1.35911311198781 1 23 -1.1831216960812512 1 24 -0.9002655818585339 1 25 -1.589057040311971 1 26 -1.2194537776897718 1 27 -1.0049635908340804 1 28 -1.0212277259468792 1 29 -1.536149440194741 1 30 -1.3408287274889545 1 31 -0.8357925865766802 1 32 -1.3673935272001358 1 33 -1.1800051351762804 1 34 -1.1310039159586864 1 35 -1.7347183803837325 1 36 -1.4351063686079613 1 37 -1.1653755482660912 1 38 -1.5289179591934285 1 39 -1.8470823105857974 1 40 -1.5863215421864467 1 41 -1.9715323284673758 1 42 -2.133429236355827 1 43 -1.9224463490381005 1 44 -1.001176824538927 1 45 -0.8834848043110242 1 46 -0.6946966970282098 1 47 -0.6099516331812538 1 48 -0.7382072460987956 1 49 -0.9633952366907224 1 50 -2.1077281608988017 1 51 -1.3482337773832924 1 52 -1.5325224075080648 1 53 -1.1475429018109509 1 54 -1.0178711380118735 1 55 -1.0761524409603354 1 56 0.3973264576688855 2 1 -0.36738653801999277 2 2 0.644953343805831 2 3 0.3795532005281004 2 4 0.46757042276131244 2 5 0.6382560257672267 2 6 0.24828205334926423 2 7 0.5255767780697767 2 8 0.1842150074185227 2 9 -0.2868838289422584 2 10 0.5444193178607648 2 11 -0.12444159942173627 2 12 -0.02682239880591908 2 13 0.2913861220419065 2 14 0.3137543286100737 2 15 -0.07290139564697284 2 16 0.2067688848496445 2 17 0.09460153895621914 2 18 0.1989373783337598 2 19 0.2017786091575715 2 20 -0.0949086271249513 2 21 -0.331563870925919 2 22 -0.3240550849085011 2 23 -0.1819127985957607 2 24 -0.02647447934402436 2 25 0.42206481655456685 2 26 0.019424365443690306 2 27 -0.41381272042789163 2 28 -0.5154250180165079 2 29 -0.6551049527672816 2 30 -0.3814476830015252 2 31 0.14704106912931536 2 32 -0.4894921732349502 2 33 -0.35287747560181515 2 34 0.18379298926375043 2 35 0.11175163895862283 2 36 -0.19042551754413495 2 37 -0.13974692157363222 2 38 -0.5743725046479753 2 39 -0.3388618076844452 2 40 0.4192393862468222 2 41 -0.36369867276138024 2 42 -0.09256850134615746 2 43 -0.5546997339510611 2 44 -0.06669906825266025 2 45 0.015451365901739678 2 46 -0.08903905679610347 2 47 -0.6034235867595896 2 48 0.20400745409462928 2 49 -0.28883043978550305 2 50 -0.14955601389686635 2 51 0.08987067135945281 2 52 -0.029379274045783887 2 53 0.8732501349395321 2 54 0.10954665695911103 2 55 0.8883059729661618 2 56 0.7333999586360065 3 1 -0.2684041143382174 3 2 0.28764529570222663 3 3 0.005362636235773266 3 4 0.2746934569888779 3 5 0.2989868305775829 3 6 0.15230610515315135 3 7 0.31998010831954815 3 8 0.15137504442130023 3 9 -0.03804016566751045 3 10 0.22897065921136317 3 11 -0.2504823024744596 3 12 -0.1845467281306128 3 13 -0.19612544132534282 3 14 0.3694897587673367 3 15 -0.24403923098461888 3 16 -0.22200077287468645 3 17 -0.03190169878661209 3 18 -0.3840875985395016 3 19 -0.28850816235028987 3 20 -0.8160002089935929 3 21 -0.22541215954756666 3 22 -0.11059532542654438 3 23 -0.09171853402591523 3 24 -0.44275570043561174 3 25 -0.5851044711420101 3 26 0.39615887053157195 3 27 0.09335656726868535 3 28 0.15663788670134693 3 29 -0.7367423255702406 3 30 -0.4771923638525877 3 31 0.6814999035418978 3 32 0.43349580086310624 3 33 0.3797774735193684 3 34 0.4855968745551422 3 35 0.298384386414682 3 36 1.304024066174648 3 37 0.9788932264374708 3 38 1.1212796692650713 3 39 0.5353287566078819 3 40 0.5297851771811466 3 41 -0.11184853400096983 3 42 -0.37008931540720624 3 43 -0.6719522778918545 3 44 -0.5095760340250912 3 45 -1.0139195869197195 3 46 -1.1951907681744558 3 47 -1.5076789059085272 3 48 -0.2920610010395066 3 49 -0.9386916793784721 3 50 -0.3898427854047313 3 51 0.10235910799219994 3 52 0.5638214660097205 3 53 -0.08627807967656409 3 54 0.14863083827270884 3 55 0.7583337250475672 3 56 -0.670855360512931 4 1 -0.07006256346416961 4 2 0.85431943286045 4 3 0.2572676115831527 4 4 0.2729005390148771 4 5 0.7910307570679507 4 6 0.013691134288066564 4 7 0.03456997833299624 4 8 0.03505949085787563 4 9 0.11682312433696751 4 10 0.3179442136712467 4 11 -0.12553833116115243 4 12 -0.14263727048830077 4 13 0.10351597507985133 4 14 -0.4923434998610787 4 15 -0.2572620010428892 4 16 -0.3017856227178086 4 17 -0.21511550425501014 4 18 -0.22418172673313563 4 19 -0.5543082519961899 4 20 -0.35253091271389536 4 21 -0.4207281613404845 4 22 0.0011258758284995452 4 23 -0.3978592780868766 4 24 -0.31052799985290847 4 25 -0.6450551658977233 4 26 -0.24850719849577274 4 27 -1.0167958307591078 4 28 -0.023102140192428067 4 29 -0.3740126079548176 4 30 -0.8792542195226929 4 31 0.051625407800074186 4 32 -0.18920702298269987 4 33 0.13571651430825682 4 34 0.022463750395573356 4 35 -0.8238702079376239 4 36 -0.26500327582445493 4 37 -0.04049269915643805 4 38 -0.8114859067098337 4 39 -1.1392566311106336 4 40 -0.5679288524020052 4 41 -0.43334113821433107 4 42 0.1321518320602948 4 43 -1.0322167332930192 4 44 -0.3180699829269287 4 45 -0.587093051733703 4 46 0.08775409959784497 4 47 -0.5489160193105889 4 48 -1.2036477334464983 4 49 -0.8436670267563287 4 50 -1.1015191637104245 4 51 -0.7990304034376514 4 52 -0.507334966083512 4 53 -0.6327818896272568 4 54 -0.796573106146834 4 55 -0.6948262928816114 4 56 0.5095570106210907 5 1 0.26363249893409346 5 2 0.5442814513086347 5 3 0.16600336850122233 5 4 -0.012720730654137924 5 5 0.507796899227472 5 6 0.22107040721265037 5 7 0.5935499023698867 5 8 0.2527480421329713 5 9 0.5031497656826005 5 10 0.2497944578383695 5 11 0.3839570567932711 5 12 0.34428622381446417 5 13 0.29060224746184105 5 14 0.2288719097970855 5 15 0.42575481935863024 5 16 0.17754156122142462 5 17 -0.11256531434725048 5 18 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0.7477902232384537 6 3 0.33963098593189756 6 4 0.1624914934545174 6 5 0.5672196496519177 6 6 0.16855201445656576 6 7 0.17657197367719135 6 8 -0.014824114063976879 6 9 0.2931893808495474 6 10 0.31849847594816083 6 11 0.022893106595198276 6 12 0.4758085780872463 6 13 0.4749825530645566 6 14 0.0026127134190403467 6 15 0.2779336535408561 6 16 -0.07520320391723345 6 17 0.40299778695439414 6 18 0.4213121209555908 6 19 -0.126750331588255 6 20 0.6505729560367968 6 21 0.2800810615838003 6 22 -0.34750509634753257 6 23 -0.0674081085934688 6 24 0.5525692177504595 6 25 0.25185205059712246 6 26 0.442325303444143 6 27 0.7456473573575124 6 28 0.628821149908454 6 29 0.2830098950936204 6 30 1.11420548560978 6 31 0.5458056538925291 6 32 -0.31790672368106954 6 33 0.541769058372104 6 34 0.11801856693476509 6 35 -0.07550528165450743 6 36 0.34446352386515855 6 37 0.01570949572217728 6 38 0.7159500759971849 6 39 0.523641052073053 6 40 0.7803695624078841 6 41 0.9866788152276413 6 42 1.3669373325591323 6 43 1.4571968572180083 6 44 1.6658564110426965 6 45 0.8342571311728477 6 46 1.8853375160705212 6 47 2.1697404489301846 6 48 2.4102531644583927 6 49 2.094033893319054 6 50 2.425140851808923 6 51 1.649811188403607 6 52 1.9145694790999492 6 53 1.393268437387487 6 54 1.7364054993292797 6 55 2.5713304524902973 6 56 1.1409725964763169 7 1 -0.16502247182614127 7 2 0.8922346676828994 7 3 0.28120804686379586 7 4 0.5176886260710063 7 5 0.7446390044906295 7 6 0.4187312259260949 7 7 0.6830235865804184 7 8 0.5392977497353044 7 9 0.4885298826416644 7 10 0.5328353935108794 7 11 0.25120851878157424 7 12 0.19516341679692317 7 13 -0.03863751862002829 7 14 -0.05007545862396512 7 15 -0.145005414559499 7 16 0.019062029057678842 7 17 0.03597269734865449 7 18 -0.10413590771824488 7 19 0.19941454296371575 7 20 -0.035806972278621174 7 21 0.00767962789204546 7 22 -0.07653735441204645 7 23 -0.01973785769741454 7 24 -0.26642719802014625 7 25 -0.6259257271806575 7 26 0.022970464734470397 7 27 -0.0435999544704444 7 28 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0.47102703181693784 120 35 0.17558142619266448 120 36 1.8734042696899138 120 37 2.2432318018306248 120 38 1.74477643152224 120 39 1.895180745507048 120 40 1.6213670458212945 120 41 0.9980034548981962 120 42 0.10955915822831486 120 43 0.4133853724319181 120 44 0.9545775274920203 120 45 0.8133237063272745 120 46 0.32585456022517434 120 47 0.9710444983467923 120 48 0.8210287306903723 120 49 0.423935517661306 120 50 0.7759759009967482 120 51 0.9090196075296681 120 52 0.8642377472308134 120 53 0.6923990711097474 120 54 0.7265679335548576 120 55 -0.06914083554447714 120 56 0.13765527778494047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 92.0 5 14.0 6 24.0 7 33.0 8 113.0 9 123.0 10 140.0 11 229.0 12 223.0 13 216.0 14 339.0 15 409.0 16 436.0 17 488.0 18 613.0 19 627.0 20 674.0 21 833.0 22 914.0 23 893.0 24 1223.0 25 1186.0 26 1028.0 27 1145.0 28 1396.0 29 1547.0 30 1855.0 31 2406.0 32 2913.0 33 3479.0 34 4599.0 35 6294.0 36 8817.0 37 14259.0 38 19815.0 39 16239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.48733508406659 0.2343611044267046 0.19460341706860296 0.08370039443810878 8 99.48972122885166 0.377480812263421 0.06901311249137336 0.06378484639354205 9 96.86722295417948 1.7797017797017798 0.5155070372461676 0.8375682288725766 10 39.95231821318777 39.23918271744359 7.972060145973189 12.836438923395447 11 26.314908923604573 35.47064851412677 20.875420875420875 17.339021686847776 12 41.888867975824496 17.441495702365266 21.25394734090386 19.415688980906374 13 22.38220716481586 16.554781772173076 27.709810318505973 33.35320074450509 14 24.72342472342472 28.251458686241293 25.480477654390697 21.544638935943283 15 34.483551874856225 20.607733651211912 24.658594223811615 20.250120250120247 16 24.076165380513206 22.852751113620677 30.617771922119747 22.453311583746366 17 34.301608214651694 21.319823493736536 20.988351423134034 23.390216868477737 18 30.88859610598741 22.78896626722714 23.26369282891022 23.05874479787523 19 23.875399962356482 22.492000752870318 30.795532969446015 22.837066315327185 20 21.62933684672815 29.87326682978857 23.708095447225883 24.789300876257396 21 22.546374720287766 21.213166865340778 32.277223581571405 23.96323483280005 22 23.597256205951858 23.29506242549721 23.67149758454106 29.436183784009874 23 25.573540790932096 30.18487148921932 21.795595708639187 22.445992011209405 24 22.971955580651233 31.648785996612084 24.392998306041786 20.9862601166949 25 24.96547683809683 20.87291291793949 25.68000167385027 28.481608570113405 26 28.901582917464403 21.23913248171746 26.450310201604886 23.40897439921325 27 22.915794322083578 19.783728330960557 26.79633196549703 30.50414538145884 28 23.439201071937024 28.881584456913156 26.46135164559082 21.217862825558996 29 29.902715382279325 22.44876587812719 27.219796215429405 20.428722524164076 30 20.95029454309134 28.23525712249219 30.871470199786167 19.9429781346303 31 27.099580712788264 23.075471698113205 29.954926624737944 19.870020964360588 32 22.212431156569632 22.31628638867034 37.696302124311565 17.774980330448468 33 25.333445268802535 23.47706547175553 33.09057328450148 18.098915974940446 34 20.72164732058521 25.13906089292836 34.94049243298839 19.198799353498035 35 18.399529643978287 30.776822367108675 35.21895702751793 15.604690961395109 36 22.900538848565695 25.838471476739176 34.327699757360584 16.933289917334537 37 19.513074077607133 25.53340328786289 37.471249748269685 17.482272886260294 38 22.151552742793466 25.146653770946788 36.63821198040411 16.063581505855637 39 19.98465306465685 23.789852101794327 36.9335561792438 19.291938654305028 40 19.456003033910267 24.41244324586261 39.53775006057286 16.59380365965426 41 17.72819088498927 25.879518579303962 39.08913016033329 17.30316037537348 42 18.697322039248697 23.61551007523544 36.79065607407797 20.89651181143789 43 18.821002537403007 25.509849545689043 37.05240105707578 18.616746859832173 44 17.800832060666703 23.276633840644585 38.09995260413924 20.822581494549475 45 18.42820426392517 25.368669419398334 38.93465071205865 17.268475604617848 46 17.860680788281165 25.41995995363052 38.34229107387501 18.3770681842133 47 20.280449646540735 24.714756792633718 37.56571392450405 17.439079636321495 48 22.08695101752313 25.707624302397956 35.80689742480668 16.398527255272235 49 21.54623302117607 26.45954610647094 35.99194296802497 16.002277904328018 50 17.4281647071229 28.774186745399906 36.83871988190015 16.958928665577055 51 17.51742463384544 28.92331052226452 37.03512342229299 16.524141421597054 52 19.607160564152874 26.42488686350834 36.432587529141216 17.535365043197572 53 17.52205292702486 27.230616637825516 36.011691216815095 19.23563921833453 54 18.028704094554666 29.157872520050653 35.2944280287041 17.518995356690585 55 19.78217968065684 27.14100277552054 34.11463005371635 18.96218749010627 56 19.24201327690473 28.499963061075874 34.14000907642136 18.11801458559804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 3.5 5 4.0 6 5.0 7 6.0 8 64.5 9 123.0 10 150.5 11 178.0 12 177.5 13 215.0 14 253.0 15 418.0 16 583.0 17 848.5 18 1114.0 19 1240.0 20 1366.0 21 1658.0 22 2197.0 23 2444.0 24 2857.5 25 3271.0 26 3553.5 27 3836.0 28 4031.0 29 4196.5 30 4167.0 31 3599.5 32 3032.0 33 3136.5 34 3241.0 35 2656.0 36 2071.0 37 1883.5 38 1884.5 39 2073.0 40 2053.5 41 2034.0 42 2283.5 43 2533.0 44 2788.0 45 3043.0 46 3135.0 47 3311.0 48 3395.0 49 3501.0 50 3607.0 51 4330.5 52 5054.0 53 5331.0 54 5668.5 55 5729.0 56 5380.0 57 5031.0 58 4889.0 59 4747.0 60 4403.5 61 4060.0 62 3820.5 63 2930.0 64 2279.0 65 2154.5 66 2030.0 67 1649.0 68 1268.0 69 1040.5 70 813.0 71 701.0 72 556.5 73 524.0 74 408.5 75 293.0 76 224.5 77 156.0 78 131.5 79 81.0 80 55.0 81 44.0 82 33.0 83 23.0 84 13.0 85 10.5 86 8.0 87 5.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05751092707614447 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0481000481000481 26 0.053328314197879416 27 0.11083924127402388 28 0.11083924127402388 29 0.1474371039588431 30 0.24259154693937304 31 0.24468285337850554 32 0.3231068448459753 33 0.3565677478720957 34 0.36806993328732457 35 0.4046677959721438 36 0.45067653763305937 37 1.3457556935817805 38 0.5353744484179267 39 0.5238722630026978 40 0.739276826233348 41 0.6085701737875651 42 0.6263462785201915 43 0.6849028588159023 44 0.7204550682811552 45 0.7298659472572516 46 0.7779659953572997 47 0.747642051989878 48 0.8835769705334923 49 0.846979107848673 50 0.8354769224334442 51 0.8333856159943117 52 0.8752117447769622 53 0.9013530752661187 54 0.9139009139009139 55 0.9170378735596127 56 0.9233117928770104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 95634.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.52320304494217 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3321046480266 33.65121191208147 2 11.880787557313674 11.054645837254533 3 5.207677784770296 7.268335529205094 4 3.101681201114807 5.772005772005772 5 1.863256315742156 4.3342325951021605 6 1.2204441247864783 3.4067381893468847 7 0.869819293356109 2.8326745718050064 8 0.6810213072012946 2.534663404228622 9 0.49671851119302346 2.079804253717297 >10 2.16443405556055 16.940627810193025 >50 0.12586532410320958 4.082230169186691 >100 0.051694686685246787 4.087458435284522 >500 0.0022475950732715995 0.6618984879854445 >1k 0.0022475950732715995 1.2934730326034674 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1237 1.2934730326034674 No Hit TCACTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 633 0.6618984879854445 No Hit TCACTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 348 0.3638873204090595 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 316 0.33042641738293915 No Hit TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 267 0.27918940962419225 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 249 0.2603676516719995 No Hit TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGT 227 0.2373632808415417 No Hit TCACTTGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA 210 0.21958717610891526 No Hit TCACTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCA 196 0.20494803103498754 No Hit TCACTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 190 0.19867411171758997 No Hit TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 183 0.19135453918062612 No Hit TCACTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 164 0.17148712800886715 No Hit TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 162 0.16939582156973462 Illumina Single End Adapter 2 (95% over 23bp) TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 158 0.16521320869146958 No Hit TCACTTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC 132 0.13802622498274672 No Hit TCACTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.1327979588849154 No Hit TCACTTGGGGAGTCTTAAGGACTGCTCTGTGTAACACTGTGACTGCCCTGTGTGCT 116 0.12129577346968652 No Hit TCACTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.12129577346968652 No Hit TCACTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 114 0.11920446703055397 No Hit TCACTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC 112 0.11711316059142146 No Hit TCACTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.11188489449359014 No Hit TCACTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 107 0.11188489449359014 No Hit TCACTTGGGAAGCAGTGGTATCAACAGCCTCGCTTGTCAAGGAAAGTATACCCCAA 103 0.10770228161532508 No Hit TCACTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.10770228161532508 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 102 0.10665662839575883 No Hit TCACTTGGGAGAAATTGCCGTTTTGTGATTCCTTTTGATTTTGTGTCTGTATAACA 100 0.10456532195662631 No Hit TCACTTGGGTGCTGGTTGATCTAGGACATTCCTCAGCCTGTGCACACAAACGCCTG 100 0.10456532195662631 No Hit TCACTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 99 0.10351966873706005 No Hit TCACTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.10247401551749377 No Hit TCACTTGGGCAGAGATCTTCAGCTTTGGATGATCCCTTTTCCATTCTGGATTTGCT 96 0.10038270907836125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.003136959658698789 0.0 0.0 0.0 0.0 9 0.006273919317397578 0.0 0.0 0.0 0.0 10 0.041826128782650525 0.0 0.0 0.0 0.0 11 0.07005876571093962 0.0 0.0 0.0 0.0 12 0.07424137858920468 0.0 0.0 0.0 0.0 13 0.07737833824790347 0.0 0.0 0.0 0.0 14 0.10874793483489135 0.0 0.0 0.0 0.0 15 0.11293054771315642 0.0 0.0 0.0 0.0 16 0.11920446703055398 0.0 0.0 0.0 0.0 17 0.1244327331283853 0.0 0.0 0.0 0.0 18 0.12861534600665037 0.0 0.0 0.0 0.0 19 0.13175230566534915 0.0 0.0 0.0 0.0 20 0.19135453918062614 0.0 0.0 0.0 0.0 21 0.19449149883932493 0.0 0.0 0.0 0.0 22 0.2174958696697827 0.0 0.0 0.0 0.0 23 0.2467741598176381 0.0 0.0 0.0 0.0 24 0.27291549030679463 0.0 0.0 0.0 0.0 25 0.27814375640462596 0.0 0.0 0.0 0.0 26 0.2833720225024573 0.0 0.0 0.0 0.0 27 0.2927829014785536 0.0 0.0 0.0 0.0 28 0.3502938285546981 0.0 0.0 0.0 0.0 29 0.3513394817742644 0.0 0.0 0.0 0.0 30 0.381663425141686 0.0 0.0 0.0 0.0 31 0.38898299767864986 0.0 0.0 0.0 0.0 32 0.39734822343517995 0.0 0.0 0.0 0.0 33 0.40675910241127633 0.0 0.0 0.0 0.0 34 0.4151243281678064 0.0 0.0 0.0 0.0 35 0.48727440031787855 0.0 0.0 0.0 0.0 36 0.4925026664157099 0.0 0.0 0.0 0.0 37 0.5050505050505051 0.0 0.0 0.0 0.0 38 0.520735303343999 0.0 0.0 0.0 0.0 39 0.5332831419787941 0.0 0.0 0.0 0.0 40 0.540602714515758 0.0 0.0 0.0 0.0 41 0.5531505531505532 0.0 0.0 0.0 0.0 42 0.5677896982244809 0.0 0.0 0.0 0.0 43 0.5782462304201434 0.0 0.0 0.0 0.0 44 0.5939310287136375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTCCC 20 3.892779E-4 50.68747 37 TCGTGTT 20 3.892779E-4 50.68747 37 CAGTACG 25 1.9118548E-5 50.687466 37 TTTACCT 15 0.007998947 50.633457 35 CAGTTTA 15 0.007998947 50.633457 35 ATATGCT 25 1.9239666E-5 50.633457 34 AGAACGC 15 0.007998947 50.633457 34 AACCTCA 15 0.007998947 50.633457 35 GAACCTC 15 0.007998947 50.633457 34 TTGCATC 30 9.512478E-7 50.633457 36 CTTTACC 15 0.007998947 50.633457 34 AAAGGCT 25 1.9239666E-5 50.633457 35 AAGGCTA 25 1.9239666E-5 50.633457 36 CTCAGTA 25 1.9239666E-5 50.633457 35 GCGGTTT 15 0.00808357 50.498936 33 TAGAACG 15 0.00808357 50.498936 33 TGAACCT 15 0.00808357 50.498936 33 ACTTTAC 15 0.00808357 50.498936 33 CTGAACC 15 0.008117605 50.44533 32 AATCTTT 15 0.008117605 50.44533 32 >>END_MODULE