FastQCFastQC Report
Fri 17 Jun 2016
SRR1526511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38284
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2870.7496604325566816No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.5720405391286176No Hit
ACGAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.4205412182635044No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1510.39442064570055374No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1320.34479155783094767No Hit
ACGAGCGGGCAGGACATATTACTGGATGAATATATTGATGCACATGGTTGTTTCCTATTTAAGC1210.316058928011702No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1130.29516246996134154No Hit
ACGAGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT1020.2664298401420959No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.2638177828858008No Hit
ACGAGCGGGTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC960.25075749660432556No Hit
ACGAGCGGGGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGATGGTTTTCTTTCTTTCCAT870.22724898129767007No Hit
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG820.21418869501619472No Hit
ACGAGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA800.20896458050360464No Hit
ACGAGCGGGAGGCCTATATTAAAAACCATAATACCAAAAAGGAAAGTCTGTAGTCCTATAATAA750.19590429422212935No Hit
ACGAGCGGGCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCT720.1880681224532442No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC700.18284400794065406No Hit
ACGAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG680.17761989342806395No Hit
ACGAGCGGGGGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCT670.17500783617176888No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC600.15672343537770347No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC540.1410510918399331No Hit
ACGAGCGGGACTGCGTAGCCCAGGCTGGTCTTGGACTTGCAGCCCTCCCCTGCCTCCAGTGTTG520.135826977327343No Hit
ACGAGCGGGAAGATTTAAAATATAAAGGTATCTAAATCGTTGTCTTATTGTAAATGTAACTCCT500.13060286281475292No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC500.13060286281475292No Hit
ACGAGCGGGAATCTAAAGGCCCTCGGCTAGTCTGATCCTTGCCCTAAGCATAGTCCCGTTAGCC500.13060286281475292No Hit
ACGAGCGGGAGCATGGTATCAACGCAGAGTCGACTTTTTGTTTTTTTTTTTTTTTTTTTTTTTT490.12799080555845782No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC470.12276669104586772No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG460.12015463378957268No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC460.12015463378957268No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC460.12015463378957268No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG450.1175425765332776No Hit
ACGAGCGGGGAGAGTCGTCACCAGCGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCGC440.11493051927698254No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGAAGGTCA430.1123184620206875No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGATTGCTCTTCCAGAGATTCT420.10970640476439245No Hit
ACGAGCGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC420.10970640476439245No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGGCTTCGCTGCGCTTCTCCTGCAGCCTGGTAGGCAC410.10709434750809736No Hit
ACGAGCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGC410.10709434750809736No Hit
ACGAGCGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAGCT400.10448229025180232No Hit
ACGAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.10187023299550726No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC390.10187023299550726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAAC150.004607338558.1432342
GGTTGTT201.961596E-458.1432345
CTATAAT150.004607338558.1432356
TCCTATA150.004607338558.1432354
CTCCCAA150.004607338558.1432357
CGCCTAG201.961596E-458.1432356
TATAATA150.004607338558.1432357
GTCAAAG201.961596E-458.1432333
TAGCCTC150.004607338558.1432350
CAATTAA201.961596E-458.1432331
CGAAGAA150.004607338558.1432336
CCTTGGG150.004607338558.1432335
GGTCAAA201.961596E-458.1432332
TCAAAGG201.961596E-458.1432334
TTCTTCC201.961596E-458.1432350
TGTAGTC150.004607338558.1432349
CTAGCCT150.004607338558.1432349
AAGCGAA150.004607338558.1432333
ACTAGCC150.004607338558.1432348
AATACCA201.961596E-458.1432330
Produced by FastQC (version 0.11.5)