FastQCFastQC Report
Fri 17 Jun 2016
SRR1526510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37071
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2400.6474063283968601No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.4720671144560438No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1670.45048690350948184No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1210.3264006905667503No Hit
ACGAGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT1170.3156105850934693No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1110.2994254268835478No Hit
ACGAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.269752636832025No Hit
ACGAGCGGGCAGGACATATTACTGGATGAATATATTGATGCACATGGTTGTTTCCTATTTAAGC840.22659221493890103No Hit
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG810.21849963583394028No Hit
ACGAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG780.21040705672897955No Hit
ACGAGCGGGTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC780.21040705672897955No Hit
ACGAGCGGGCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCT700.18882684578241754No Hit
ACGAGCGGGGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGATGGTTTTCTTTCTTTCCAT690.18612931941409727No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC640.172641687572496No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.172641687572496No Hit
ACGAGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA630.16994416120417577No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC570.15375900299425427No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG530.14296889752097328No Hit
ACGAGCGGGGGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCT530.14296889752097328No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC490.13217879204769226No Hit
ACGAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG490.13217879204769226No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG440.11869116020609101No Hit
ACGAGCGGGACTGCGTAGCCCAGGCTGGTCTTGGACTTGCAGCCCTCCCCTGCCTCCAGTGTTG440.11869116020609101No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC430.11599363383777077No Hit
ACGAGCGGGAATCTAAAGGCCCTCGGCTAGTCTGATCCTTGCCCTAAGCATAGTCCCGTTAGCC430.11599363383777077No Hit
ACGAGCGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC420.11329610746945051No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT410.11059858110113027No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGATTGCTCTTCCAGAGATTCT400.10790105473281002No Hit
ACGAGCGGGAGGCCTATATTAAAAACCATAATACCAAAAAGGAAAGTCTGTAGTCCTATAATAA400.10790105473281002No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC400.10790105473281002No Hit
ACGAGCGGGGATTGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTTCCGGAGCG380.10250600199616952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG150.00453793558.36239633
GACGGAT150.00453793558.36239634
CTACCCG150.00453793558.36239634
AACTCCT150.00456251158.28299358
CTCGGAA150.00456251158.28299354
ATGTAAC150.00456251158.28299354
TGTAACT150.00456251158.28299355
CAATGGT150.00458718658.20380428
TGGTAGT150.00458718658.20380431
ACTTGCA150.00458718658.20380435
GTATCTA150.00458718658.20380428
GGATTCG150.00458718658.20380437
GTTTTGC150.00458718658.20380428
CGGATTC150.00458718658.20380436
TCTAAAT150.00458718658.20380431
AAGACGG150.00458718658.20380435
AATGGTA150.00458718658.20380429
CTAAATC150.00458718658.20380432
ATCGTTG150.00458718658.20380436
TACCCGT150.00458718658.20380435
Produced by FastQC (version 0.11.5)