##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526510_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37071
Sequences flagged as poor quality	0
Sequence length	64
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.09303768444337	39.0	38.0	40.0	35.0	40.0
2	37.79196676647514	39.0	38.0	40.0	35.0	40.0
3	37.742359256561734	39.0	38.0	40.0	34.0	40.0
4	37.16516953953225	39.0	37.0	39.0	33.0	40.0
5	37.59313209786626	39.0	38.0	40.0	33.0	40.0
6	37.3711256777535	39.0	38.0	40.0	33.0	40.0
7	38.15122332820803	39.0	38.0	40.0	35.0	40.0
8	38.0028863532141	39.0	38.0	40.0	35.0	40.0
9	38.19837608912627	39.0	38.0	40.0	35.0	40.0
10	36.876318416012516	39.0	35.0	40.0	31.0	40.0
11	36.9100644708802	39.0	35.0	40.0	32.0	40.0
12	36.90545170079037	39.0	36.0	40.0	32.0	40.0
13	36.89922041487956	39.0	36.0	39.0	32.0	40.0
14	36.82563189555178	39.0	36.0	39.0	32.0	40.0
15	36.74316851447223	39.0	36.0	39.0	32.0	40.0
16	36.42229775296054	38.0	36.0	39.0	31.0	40.0
17	36.09864853928947	38.0	35.0	39.0	30.0	40.0
18	36.29198025410698	38.0	35.0	39.0	30.0	40.0
19	36.17024088910469	38.0	35.0	39.0	30.0	40.0
20	35.99201532194977	38.0	35.0	39.0	30.0	40.0
21	36.0130020770953	38.0	35.0	39.0	30.0	40.0
22	35.874511073345744	38.0	35.0	39.0	29.0	40.0
23	35.64853928947156	38.0	35.0	39.0	29.0	40.0
24	35.47034069758032	38.0	35.0	39.0	29.0	40.0
25	35.48795554476545	38.0	35.0	39.0	29.0	40.0
26	35.64319818726228	38.0	35.0	39.0	29.0	40.0
27	35.099349896145235	38.0	35.0	39.0	27.0	40.0
28	34.97421164791886	38.0	34.0	39.0	27.0	40.0
29	34.78781257586793	38.0	34.0	39.0	26.0	40.0
30	34.74057888915864	38.0	34.0	39.0	27.0	40.0
31	34.247875697984945	38.0	33.0	39.0	24.0	40.0
32	34.43405896792641	38.0	33.0	39.0	25.0	40.0
33	34.38747268754552	38.0	33.0	39.0	25.0	40.0
34	33.700439696798036	38.0	33.0	39.0	23.0	40.0
35	33.68792317444903	37.0	33.0	39.0	23.0	40.0
36	32.91945186264196	37.0	32.0	39.0	17.0	40.0
37	32.38485608696825	36.0	31.0	39.0	15.0	40.0
38	32.48425993364085	36.0	31.0	39.0	16.0	40.0
39	31.896334061665453	36.0	30.0	39.0	10.0	40.0
40	31.962045803997736	36.0	31.0	39.0	10.0	40.0
41	31.332874753850717	36.0	30.0	39.0	4.0	40.0
42	30.96142537293302	36.0	29.0	39.0	4.0	40.0
43	30.965390736694452	35.0	29.0	39.0	4.0	40.0
44	30.773272908742683	35.0	29.0	39.0	4.0	40.0
45	30.478082598257398	35.0	29.0	39.0	4.0	40.0
46	29.939656335140675	35.0	28.0	39.0	4.0	40.0
47	29.756602195786463	35.0	28.0	39.0	4.0	40.0
48	29.61179898033503	35.0	27.0	39.0	4.0	40.0
49	29.329745623263467	35.0	27.0	39.0	4.0	40.0
50	29.011437511801677	35.0	26.0	38.0	4.0	40.0
51	28.405654015268	34.0	24.0	38.0	4.0	39.0
52	28.296431172614714	34.0	24.0	38.0	4.0	39.0
53	28.048960103585014	34.0	23.0	38.0	4.0	39.0
54	27.594049256831486	33.0	21.0	38.0	4.0	39.0
55	27.438401985379407	33.0	20.0	38.0	4.0	39.0
56	26.53616034096733	33.0	4.0	38.0	4.0	39.0
57	26.09425157130911	33.0	4.0	37.0	4.0	39.0
58	25.730732917914274	32.0	4.0	37.0	4.0	39.0
59	25.373931105176553	32.0	4.0	36.0	4.0	39.0
60	24.996735993094333	32.0	4.0	36.0	4.0	39.0
61	24.68169188853821	32.0	4.0	36.0	4.0	39.0
62	24.339915297672036	32.0	4.0	36.0	4.0	39.0
63	24.13290712416714	32.0	4.0	36.0	4.0	39.0
64	23.962882037171912	31.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	-0.014532219478553543
2	2	0.5372841915986868
2	3	0.2848285139490443
2	4	0.2249020100673107
2	5	0.3373297825024153
2	6	-0.4691439042779706
2	7	0.5160441681042442
2	8	0.5043734907609618
2	9	0.5188478256470717
2	10	0.8389988375740458
2	11	-1.3277122241143005
2	12	0.26027427390280167
2	13	-0.5228036961432352
2	14	-0.6526349216916927
2	15	-0.2549131090578669
2	16	0.17093801533329156
2	17	-0.23895009276675694
2	18	-1.2383996112845068
2	19	-0.35206363195021595
2	20	0.2391042114737587
2	21	0.07220282914720855
2	22	-1.1087959706808164
2	23	-0.12150782725262843
2	24	-0.10576018016517708
2	25	-1.7529197142815818
2	26	-1.6928140840685728
2	27	-0.929646576393182
2	28	-1.3031047075735671
2	29	-1.2095757107554093
2	30	-0.8157686536550699
2	31	-1.9602373095276633
2	32	-2.219241655122417
2	33	-2.692328171201172
2	34	-1.984185873709393
2	35	-2.1670780315514833
2	36	-0.3659705523957939
2	37	-0.6411832251676621
2	38	-0.018001100753735955
2	39	-2.7615619915154106
2	40	-1.9347447666775963
2	41	-0.2278074440756015
2	42	-0.6809017973760199
2	43	-2.0308459084223145
2	44	-0.9568148677598529
2	45	-1.2108162392221935
2	46	0.01810050491310733
2	47	0.12947433857932822
2	48	-0.46080556663414285
2	49	-0.30040524236427046
2	50	-1.2159820442246918
2	51	-1.4425803939801867
2	52	0.07716899809113187
2	53	-0.4934292364319752
2	54	-2.8317849087932565
2	55	-0.5503855822192598
2	56	-1.087767894323239
2	57	-1.2518434524208395
2	58	-2.187804566278274
2	59	-1.5866084639180151
2	60	-1.0875159160187202
2	61	-2.795507366336043
2	62	-1.417763160054328
2	63	-2.52300161265574
2	64	-2.365730430603662
3	1	0.29381692663150716
3	2	1.1369046849573223
3	3	0.8607298422222911
3	4	1.0749968867276536
3	5	0.27471118667698846
3	6	1.1029623575816103
3	7	0.7437481529239776
3	8	0.8696486330759541
3	9	0.5966466871271479
3	10	1.4016174333994726
3	11	0.8591947967585654
3	12	0.016441256825004302
3	13	0.38706347653229045
3	14	0.36539164377320077
3	15	0.9433791110559824
3	16	0.6197046187488553
3	17	1.366363000212374
3	18	0.9058129124346621
3	19	0.7646346602698983
3	20	0.6480226175458625
3	21	0.23064685191761214
3	22	-0.08317927238859824
3	23	-0.5142972010666682
3	24	0.5858906737247622
3	25	-0.7159178167483731
3	26	0.7806204510357873
3	27	0.44796253176620837
3	28	0.7671040210791844
3	29	1.3217335871573042
3	30	0.5314799231950289
3	31	1.149819616468541
3	32	0.22857618168972493
3	33	1.2478995327836486
3	34	1.4740683957403249
3	35	0.2257113422625565
3	36	-5.352687819947974
3	37	-4.965661403535783
3	38	-5.790297115934003
3	39	-20.093630302710856
3	40	-6.449925032332246
3	41	-4.965947861532907
3	42	-5.59171773665496
3	43	-6.61054230310922
3	44	-5.619053956943915
3	45	-5.148197643396767
3	46	-0.0843662882557723
3	47	-0.7557249024073869
3	48	0.9519079058516233
3	49	-1.285224976709621
3	50	-1.3744260669950918
3	51	-0.3685765989137728
3	52	-1.3032864098785062
3	53	-1.3236000144205917
3	54	-1.6961112845048305
3	55	-1.1794178402837758
3	56	-1.0555098298071108
3	57	-0.27746015071305763
3	58	-0.12613473705626177
3	59	-1.6008399629692462
3	60	-1.6368517793963306
3	61	-4.877101294229782
3	62	-3.4670990234319383
3	63	-3.352223624040942
3	64	-3.098178248440476
4	1	0.2324723888163831
4	2	0.35203073537748963
4	3	0.9355197581886756
4	4	1.2497868026940395
4	5	0.9419380774332922
4	6	1.329012777749682
4	7	0.9824036151088507
4	8	1.253682246521329
4	9	0.7529492081355471
4	10	0.7772476855003134
4	11	0.5810435362543629
4	12	0.7635000803544187
4	13	-0.10621383439207932
4	14	0.04690424881521693
4	15	1.2114463379467395
4	16	1.0188642826144019
4	17	1.4117411514728744
4	18	0.41505660991364834
4	19	0.5705170132110737
4	20	1.2354175755290626
4	21	1.6012350872117267
4	22	1.1815266099643438
4	23	-1.4588350161927224
4	24	0.5900923543970293
4	25	0.9521494101423826
4	26	1.8251582661618428
4	27	1.3689709351275496
4	28	1.31164183620524
4	29	2.259548713207721
4	30	2.034000931598385
4	31	2.363264994619797
4	32	2.2369795430342663
4	33	2.458823902531549
4	34	3.066505370530237
4	35	-1.5726079854685295
4	36	3.4607575582032837
4	37	3.395683134279345
4	38	2.1626440605365858
4	39	3.1988066720790584
4	40	4.055116984474477
4	41	3.282791634265415
4	42	4.59651755746269
4	43	4.294499713697501
4	44	4.518761169106508
4	45	4.470289751561214
4	46	4.199667325189605
4	47	4.925787702634629
4	48	4.730899502490278
4	49	4.252590149340801
4	50	3.912128554853652
4	51	4.510414997724883
4	52	4.6605791363399796
4	53	4.621778136839911
4	54	3.839182833142228
4	55	1.7205821597162227
4	56	3.4016330273357447
4	57	2.6006911097911427
4	58	3.4444534982378556
4	59	3.194958356358484
4	60	2.3152490609398058
4	61	2.1464281175349242
4	62	2.356430388332768
4	63	2.241053686883429
4	64	3.2093847767696104
5	1	-0.5805146241706325
5	2	-0.1215623382155826
5	3	-0.5519261292572111
5	4	-0.5952348423276064
5	5	-0.8268930913978778
5	6	-0.6822426335057301
5	7	-0.41846218575695104
5	8	-0.35584156300247827
5	9	-0.4782196230332829
5	10	-0.78465707640445
5	11	-1.2094326542218283
5	12	-0.5940756772213405
5	13	-0.5140060421842847
5	14	-0.2362126343016655
5	15	-0.0985103720099687
5	16	-17.227888964138845
5	17	-1.7120683723366454
5	18	-1.8464152515582128
5	19	-2.120392077698021
5	20	-1.8234568833454006
5	21	-1.5260376400609985
5	22	-1.969988541550805
5	23	-1.9670601244178272
5	24	-1.115535351230676
5	25	-0.33356487557190206
5	26	1.3394439804475553
5	27	1.2590142251708443
5	28	1.0683517929151947
5	29	1.4482933019523117
5	30	1.6045637021611583
5	31	-0.16747093611613195
5	32	0.37117867723340225
5	33	0.8423736860813307
5	34	1.815423119447992
5	35	-1.9275863404468865
5	36	0.511839809285533
5	37	0.4086701472663563
5	38	0.7747652726577954
5	39	0.4749971482695372
5	40	0.6204849498424387
5	41	-3.3812776298038507
5	42	-19.336815775870647
5	43	-4.843162623964837
5	44	-3.1894639391186033
5	45	-3.1375024562309903
5	46	-4.021977696455416
5	47	-3.145207968361042
5	48	-3.6318710602802824
5	49	-4.037453140702489
5	50	-3.1025900598649656
5	51	1.062795950105837
5	52	1.0077652835261262
5	53	0.5222110372728146
5	54	1.1603949543543486
5	55	0.7448245839586498
5	56	0.3081265338292525
5	57	-0.2391357300356951
5	58	1.0262716800560376
5	59	-0.3236563622562336
5	60	1.0503139960047392
5	61	0.8858220569288626
5	62	0.6594606913630727
5	63	1.624603470433211
5	64	0.878648846033677
6	1	0.03247238881638026
6	2	0.49488787823463554
6	3	-0.8930516703827536
6	4	-0.3502131973059619
6	5	0.11336664886186298
6	6	0.44329849203539595
6	7	0.4966893293945631
6	8	0.5679679608070458
6	9	0.49580635099269443
6	10	1.6343905426431746
6	11	0.6096149648257949
6	12	0.19207150892584934
6	13	0.17950045132220538
6	14	-0.2959528940419247
6	15	1.4114463379467423
6	16	-1.6668500030998885
6	17	0.35459829433001744
6	18	-0.15637196151492105
6	19	0.256231298925357
6	20	-2.078868138756654
6	21	-1.0844791985025566
6	22	-1.4756162471785146
6	23	-1.1731207304784377
6	24	-1.1527647884601109
6	25	-1.5621363041433298
6	26	0.42515826616184427
6	27	0.6832566494132664
6	28	0.4544989790623788
6	29	0.7738344274934335
6	30	0.4340009315983906
6	31	1.1061221374769445
6	32	0.008408114462838512
6	33	-0.6554618117541651
6	34	-0.047780343755476906
6	35	-0.5440365568971046
6	36	-4.967813870368143
6	37	-4.51860258000637
6	38	-3.8659273680348427
6	39	-19.54405047077808
6	40	-6.687740158382667
6	41	-6.974351222877445
6	42	-6.574911013965885
6	43	-6.162643143445354
6	44	-5.76695311660778
6	45	-5.958281677010209
6	46	-0.02890410338182292
6	47	0.3829305597774848
6	48	0.21661378820456534
6	49	0.10973300648365836
6	50	0.5692714119965068
6	51	1.8532721405820247
6	52	1.746293422054265
6	53	2.1646352796970554
6	54	0.6106114045708004
6	55	1.7491535882876512
6	56	2.173061598764317
6	57	2.4578339669340004
6	58	2.2730249268092813
6	59	2.6521012135013393
6	60	2.8581062037969467
6	61	1.6892852603920687
6	62	2.0707161026184835
6	63	1.9553394011691445
6	64	1.6379562053410375
7	1	-0.3077873514433591
7	2	0.15116493451169077
7	3	0.5995890222579447
7	4	-0.29220453929730184
7	5	-0.4935597580645492
7	6	-0.22769717896027686
7	7	-0.4790682463630134
7	8	-0.35584156300247827
7	9	-0.4782196230332829
7	10	-0.2998085915559656
7	11	-0.11852356331273484
7	12	-0.5334696166152781
7	13	-0.968551496729745
7	14	-1.115000513089548
7	15	-0.5227527962523979
7	16	-0.3491010853509664
7	17	-0.8029774632457389
7	18	-0.8161122212551817
7	19	-0.5749375322434744
7	20	-1.0355780954666116
7	21	-0.435128549151905
7	22	-0.1821097536720231
7	23	-0.2094843668420694
7	24	-2.4185656542609735
7	25	-2.121443663450691
7	26	-1.4181317771282025
7	27	-1.2864403202837025
7	28	-1.8407391161757118
7	29	-1.7941309404719306
7	30	-1.8802847826873261
7	31	-5.743228511873706
7	32	-5.295487989433266
7	33	-5.884899041191396
7	34	-22.82094051691565
7	35	-6.685162098022644
7	36	-8.276038978593252
7	37	-8.28829954970334
7	38	-8.437355939463416
7	39	-7.797730124457736
7	40	-8.046181716824226
7	41	-3.3509745995008196
7	42	-3.276209715264585
7	43	-3.9037686845708954
7	44	-2.5531003027549666
7	45	-2.3799266986552325
7	46	-3.143189817667537
7	47	-2.5997534229064954
7	48	-2.086416514825739
7	49	-2.279877383126731
7	50	-2.0722870295619344
7	51	-2.5735676862577996
7	52	-2.1740528982920537
7	53	-2.5080919930302166
7	54	-3.0820292880698936
7	55	-1.7400239008898382
7	56	-3.419146193443474
7	57	-3.3300448209447886
7	58	-3.519182865398509
7	59	-2.7782018168016904
7	60	-3.4648375191467764
7	61	-2.8717537006468916
7	62	-2.5223574904551107
7	63	-2.587517741688
7	64	-2.9395329721481396
8	1	-0.27748432114032795
8	2	0.3632861466329018
8	3	0.6298920525609688
8	4	-0.14068938778215312
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118	11	-0.9937463797120216
118	12	0.1340883156485333
118	13	-0.23058358229123854
118	14	-0.07578482681503829
118	15	-0.23309147717931467
118	16	-1.2038247930158548
118	17	0.04283358844766383
118	18	-0.68242238168299
118	19	-0.7941888691418697
118	20	-0.4108009118658984
118	21	-1.563470795141214
118	22	-1.2596498606238882
118	23	-0.8672383775372623
118	24	-0.9435210909811218
118	25	-1.480623699101315
118	26	-0.7782030783759737
118	27	-0.2873315858808496
118	28	-0.7917195083325765
118	29	-0.3841487657838769
118	30	-0.8214612532755581
118	31	0.0027607929391280095
118	32	-0.330247347722036
118	33	-0.10504164368693836
118	34	0.2681860427991509
118	35	0.0786525187331506
118	36	-0.49974664347738695
118	37	-0.9950731682416638
118	38	0.35676170759541037
118	39	-0.06421853800497601
118	40	0.6383102617854028
118	41	-0.4659478615329071
118	42	0.025929322168572355
118	43	0.7129871086554864
118	44	-0.2955245451792088
118	45	0.08709647425029488
118	46	0.9156337117442277
118	47	1.332510391710258
118	48	1.3048490823222139
118	49	1.3030103174080274
118	50	1.4196915800637342
118	51	2.5725998716744627
118	52	2.6378900607097293
118	53	2.117576456167644
118	54	2.862712244906934
118	55	2.3794056891279887
118	56	0.8562548760752406
118	57	0.7519516139928228
118	58	1.5503358511790317
118	59	1.4873953311483987
118	60	2.5984423382507273
118	61	2.3876045881231605
118	62	1.5917245059798262
118	63	2.059541081841413
118	64	1.7547629280301145
119	1	-0.01990856356457016
119	2	0.8885386718854278
119	3	0.6172657899347058
119	4	0.6370883899956254
119	5	0.46098569648091114
119	6	1.0702826190195225
119	7	0.7633559960612288
119	8	0.8386028814419646
119	9	0.5950127001990424
119	10	0.2708984791511071
119	11	0.2627895680003931
119	12	0.5801667470210816
119	13	0.5668020386237913
119	14	-0.6983338464228765
119	15	0.7146209411213391
119	16	-0.770818257068143
119	17	0.5379316276633546
119	18	0.3682312130882508
119	19	-0.2794829867889277
119	20	-0.5153760752645908
119	21	0.9234573094339495
119	22	0.4870821655199009
119	23	-0.07312073047843626
119	24	-0.5170505027458248
119	25	-0.2224537644607878
119	26	-0.6605560195524447
119	27	-1.3722989061422908
119	28	-0.06296133839793328
119	29	-1.0214036677446572
119	30	-1.8802847826873261
119	31	-2.0462588149040073
119	32	-1.4495283934736705
119	33	-2.7586364149287697
119	34	-0.4573041532792814
119	35	-2.3821317949923433
119	36	-2.298766251320526
119	37	-3.096380357784149
119	38	-2.992911495018973
119	39	-2.6083361850637985
119	40	-1.9956766663191736
119	41	-2.5656210641472867
119	42	-3.1979268869817545
119	43	-2.5451828259850373
119	44	-3.378857878512541
119	45	-3.4178054865340215
119	46	-2.4209675954453154
119	47	-2.291672614825689
119	48	-2.9778306562398775
119	49	-2.3783622316115824
119	50	-1.7061254134003185
119	51	-0.937204049894163
119	52	-0.8179922922314482
119	53	-0.4853647203029432
119	54	-1.1552616113022154
119	55	-0.7071956180615544
119	56	-2.0832876075848894
119	57	-2.431054921954889
119	58	-3.1833242795399244
119	59	-2.525676564276438
119	60	-3.0911001454094027
119	61	-2.220238549131743
119	62	-2.226902945000564
119	63	-2.254184408354668
119	64	-2.8612501438653126
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	37.0
5	5.0
6	16.0
7	13.0
8	39.0
9	48.0
10	77.0
11	70.0
12	136.0
13	140.0
14	178.0
15	195.0
16	260.0
17	273.0
18	310.0
19	360.0
20	421.0
21	582.0
22	725.0
23	694.0
24	758.0
25	706.0
26	704.0
27	797.0
28	918.0
29	1051.0
30	1192.0
31	1378.0
32	1634.0
33	2044.0
34	2677.0
35	3132.0
36	3823.0
37	4534.0
38	4514.0
39	2624.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.13409403576921	0.6420112756602196	0.10520352836448978	0.11869116020609101
8	99.3901122625216	0.3292314335060449	0.15921848013816925	0.12143782383419689
9	98.49747781284563	0.7822826468128726	0.28863532141026677	0.43160421893124007
10	55.954789458066955	27.110140001618515	5.502953791373311	11.43211674894122
11	36.34107523401041	26.82420220657657	16.090744787030292	20.743977772382724
12	38.890237652073054	16.352404844757356	24.43958889698147	20.317768606188125
13	28.28356397183783	15.94777588950932	29.775296053518925	25.99336408513393
14	24.593347899975722	19.956300072833212	33.58690081195544	21.863451215235628
15	27.33776050102581	18.356548968793867	31.937695713205915	22.36799481697441
16	29.889119702160954	19.68867186446165	25.82890441632719	24.593304017050208
17	31.080205238995408	20.024304617877398	21.39076424520659	27.504725897920608
18	32.82889590245745	21.879636373445553	21.879636373445553	23.41183135065145
19	32.04391572927625	20.71970003506784	22.796795338674436	24.43958889698147
20	26.688970429527302	25.77703431901576	22.318152385063676	25.215842866393267
21	28.375279868360714	20.935502144533462	26.745973941895286	23.943244045210545
22	28.4265328693588	20.511990504707185	25.14094575274473	25.920530873189286
23	30.54948612122684	21.499285155512396	26.179493404548033	21.77173531871274
24	30.314801327182973	26.338647460278924	20.611798980335035	22.73475223220307
25	31.066310420208893	20.44098993333873	22.618951232019	25.87374841443338
26	26.68159620106306	18.10700698810134	24.952108571891106	30.2592882389445
27	24.616798680760184	17.436673785515396	29.22062123219161	28.725906301532806
28	26.39699524427151	21.489948119325554	24.378512753999136	27.734543882403806
29	26.661445815667957	22.54867543409576	25.467851259755342	25.32202749048095
30	26.202312763428075	20.790554414784392	29.62822868258943	23.3789041391981
31	28.54014598540146	18.188699648553662	32.303325223033255	20.967829143011624
32	29.441322671277288	17.65993084071753	31.753836178949648	21.144910309055543
33	30.14354066985646	17.824993917768225	31.71410807450058	20.317357337874732
34	25.020977128163484	20.002706726214644	37.028014616321556	17.948301529300313
35	23.685918234912396	25.91661258922777	33.70646766169154	16.69100151416829
36	26.917248471072142	23.791741083509226	29.920982843535203	19.37002760188342
37	25.31258457374831	25.40189445196211	31.83761840324763	17.447902571041947
38	28.37618403247632	22.257104194857916	30.606224627875505	18.760487144790257
39	26.36321689330402	21.100344705914285	31.37638085932199	21.160057541459707
40	27.80409887105071	19.79857595365081	32.08165254352004	20.315672631778433
41	22.567977467229987	20.869894919293685	33.18437872386524	23.377748889611095
42	21.09897851356111	19.87698810523749	35.77099195274609	23.253041428455308
43	23.507412124989838	17.450337407517818	36.71102197891542	22.331228488576926
44	24.638976943292963	18.431818797583247	34.24638976943293	22.682814489690863
45	22.037343161431938	19.67155361643316	33.20506219343649	25.086041028698407
46	22.193607850578765	20.683672639540244	34.0941744151373	23.02854509474369
47	21.746320041204694	21.602645775163328	36.31163761554935	20.339396568082627
48	24.897007805724197	21.330225498699047	34.34247614917606	19.430290546400695
49	24.95323302155348	19.913243866070218	33.880981428765075	21.252541683611224
50	21.58751696065129	21.26729986431479	33.27544097693351	23.869742198100408
51	19.712581344902386	23.156182212581346	35.50976138828633	21.621475054229936
52	22.630865202061294	23.417412530512614	33.89747762408462	20.05424464334147
53	23.78872551679236	20.476371330909878	32.844121317345774	22.890781834951984
54	20.781686511703597	20.109034690390303	34.49781659388647	24.611462204019638
55	25.537225960494897	19.60332103321033	30.838940742348598	24.02051226394617
56	19.751411202778986	22.413699522362137	31.236430742509768	26.598458532349113
57	21.754347885069322	25.37374175868899	31.291206555064164	21.580703801177524
58	21.76639878366638	26.04528670721112	30.72056907037359	21.467745438748914
59	25.651678071032908	25.089605734767023	29.8251330509395	19.433583143260563
60	26.27433940743557	23.001928142736876	29.677103983923093	21.046628465904462
61	24.097889196375277	22.773888979326063	27.882684898800804	25.24553692549786
62	21.623015334509432	22.689645813543223	29.157280499389334	26.53005835255801
63	21.93089596395516	22.17517574573189	27.845180902749505	28.048747387563445
64	23.70215424737171	22.150987476569505	28.445301676129418	25.701556599929372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	9.0
11	17.0
12	17.0
13	29.5
14	42.0
15	53.5
16	97.0
17	129.0
18	180.5
19	232.0
20	273.0
21	295.5
22	277.0
23	412.5
24	690.5
25	833.0
26	845.0
27	904.5
28	952.0
29	939.0
30	830.0
31	734.0
32	725.0
33	716.0
34	626.5
35	492.5
36	448.0
37	454.0
38	459.0
39	458.0
40	516.5
41	539.0
42	503.0
43	500.0
44	497.0
45	499.5
46	502.5
47	503.0
48	628.5
49	737.0
50	720.0
51	800.5
52	873.5
53	866.0
54	1014.5
55	1294.5
56	1426.0
57	1620.5
58	1815.0
59	1822.5
60	2102.0
61	2374.0
62	2477.0
63	2689.5
64	2799.0
65	2508.5
66	2070.0
67	1922.0
68	1657.5
69	1393.0
70	1165.5
71	813.0
72	688.0
73	627.0
74	455.0
75	344.0
76	299.5
77	200.5
78	146.0
79	124.0
80	82.5
81	63.0
82	51.5
83	40.0
84	32.5
85	16.0
86	7.0
87	7.0
88	5.5
89	4.0
90	2.5
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.040462895524803755
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.07283321194464676
16	0.010790105473281
17	0.11059858110113027
18	0.0
19	0.0
20	0.018882684578241753
21	0.0
22	0.0
23	0.0
24	0.0
25	0.048555474629764506
26	0.021580210946562
27	0.21580210946562003
28	0.16994416120417577
29	0.10790105473281002
30	0.15915405573089478
31	0.21849963583394028
32	0.14836395025761379
33	0.21040705672897955
34	0.33988832240835154
35	0.23468479404386175
36	0.3156105850934693
37	0.3264006905667503
38	0.3264006905667503
39	0.6150360119770171
40	0.3614685333549136
41	0.3965363761430768
42	0.4423943244045211
43	0.4639745353510831
44	0.43699927166788055
45	0.45857948261444254
46	0.4909497990342856
47	0.4909497990342856
48	0.4720671144560438
49	0.5017399045075666
50	0.5961533273987754
51	0.5152275363491678
52	0.5422028000323703
53	0.5637830109789324
54	0.5449003264006905
55	0.5799681691888537
56	0.6015483801354159
57	0.5772706428205335
58	0.6447088020285399
59	0.6554989075018208
60	0.6689865393434221
61	0.5745731164522133
62	0.6096409592403765
63	0.6150360119770171
64	0.701356855763265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	37071.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.10585093469289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.45579114380499	45.14040624747107
2	7.687869609115823	8.626689325888162
3	3.740564450213953	6.296026543659464
4	2.2212606375306505	4.985028728655823
5	1.4952641953940093	4.194653502737989
6	1.0529352372710226	3.544549647972809
7	0.7692677532573682	3.0212295325186806
8	0.4615606519544209	2.0717002508699522
9	0.40867349391797686	2.0636076717649914
>10	1.615462281840473	15.079172398910199
>50	0.06250300495216116	2.4655391006447087
>100	0.028847540747151307	2.511397048906153
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC	240	0.6474063283968601	No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	175	0.4720671144560438	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT	167	0.45048690350948184	No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG	121	0.3264006905667503	No Hit
ACGAGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT	117	0.3156105850934693	No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC	111	0.2994254268835478	No Hit
ACGAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	100	0.269752636832025	No Hit
ACGAGCGGGCAGGACATATTACTGGATGAATATATTGATGCACATGGTTGTTTCCTATTTAAGC	84	0.22659221493890103	No Hit
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG	81	0.21849963583394028	No Hit
ACGAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG	78	0.21040705672897955	No Hit
ACGAGCGGGTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC	78	0.21040705672897955	No Hit
ACGAGCGGGCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCT	70	0.18882684578241754	No Hit
ACGAGCGGGGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGATGGTTTTCTTTCTTTCCAT	69	0.18612931941409727	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC	64	0.172641687572496	No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.172641687572496	No Hit
ACGAGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA	63	0.16994416120417577	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC	57	0.15375900299425427	No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG	53	0.14296889752097328	No Hit
ACGAGCGGGGGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCT	53	0.14296889752097328	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC	49	0.13217879204769226	No Hit
ACGAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG	49	0.13217879204769226	No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG	44	0.11869116020609101	No Hit
ACGAGCGGGACTGCGTAGCCCAGGCTGGTCTTGGACTTGCAGCCCTCCCCTGCCTCCAGTGTTG	44	0.11869116020609101	No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	43	0.11599363383777077	No Hit
ACGAGCGGGAATCTAAAGGCCCTCGGCTAGTCTGATCCTTGCCCTAAGCATAGTCCCGTTAGCC	43	0.11599363383777077	No Hit
ACGAGCGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC	42	0.11329610746945051	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT	41	0.11059858110113027	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGATTGCTCTTCCAGAGATTCT	40	0.10790105473281002	No Hit
ACGAGCGGGAGGCCTATATTAAAAACCATAATACCAAAAAGGAAAGTCTGTAGTCCTATAATAA	40	0.10790105473281002	No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	40	0.10790105473281002	No Hit
ACGAGCGGGGATTGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTTCCGGAGCG	38	0.10250600199616952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.00269752636832025	0.0	0.0	0.0	0.0
11	0.00269752636832025	0.0	0.0	0.0	0.0
12	0.00269752636832025	0.0	0.0	0.0	0.0
13	0.0053950527366405	0.0	0.0	0.0	0.0
14	0.008092579104960751	0.0	0.0	0.0	0.0
15	0.008092579104960751	0.0	0.0	0.0	0.0
16	0.013487631841601252	0.0	0.0	0.0	0.0
17	0.013487631841601252	0.0	0.0	0.0	0.0
18	0.013487631841601252	0.0	0.0	0.0	0.0
19	0.013487631841601252	0.0	0.0	0.0	0.0
20	0.013487631841601252	0.0	0.0	0.0	0.0
21	0.016185158209921502	0.0	0.0	0.0	0.0
22	0.029672790051522752	0.0	0.0	0.0	0.0
23	0.029672790051522752	0.0	0.0	0.0	0.0
24	0.029672790051522752	0.0	0.0	0.0	0.0
25	0.03506784278816325	0.0	0.0	0.0	0.0
26	0.03776536915648351	0.0	0.0	0.0	0.0
27	0.040462895524803755	0.0	0.0	0.0	0.0
28	0.04585794826144426	0.0	0.0	0.0	0.0
29	0.04585794826144426	0.0	0.0	0.0	0.0
30	0.05395052736640501	0.0	0.0	0.0	0.0
31	0.05664805373472526	0.0	0.0	0.0	0.0
32	0.059345580103045505	0.0	0.0	0.0	0.0
33	0.06204310647136576	0.0	0.0	0.0	0.0
34	0.06474063283968601	0.0	0.0	0.0	0.0
35	0.06743815920800626	0.0	0.0	0.0	0.0
36	0.06743815920800626	0.0	0.0	0.0	0.0
37	0.07283321194464676	0.0	0.0	0.0	0.0
38	0.07283321194464676	0.0	0.0	0.0	0.0
39	0.07283321194464676	0.0	0.0	0.0	0.0
40	0.07283321194464676	0.0	0.0	0.0	0.0
41	0.08362331741792776	0.0	0.0	0.0	0.0
42	0.08901837015456826	0.0	0.0	0.0	0.0
43	0.08901837015456826	0.0	0.0	0.0	0.0
44	0.09171589652288852	0.0	0.0	0.0	0.0
45	0.09441342289120876	0.0	0.0	0.0	0.0
46	0.10250600199616951	0.0	0.0	0.0	0.0
47	0.11059858110113026	0.0	0.0	0.0	0.0
48	0.11329610746945051	0.0	0.0	0.0	0.0
49	0.11869116020609101	0.0	0.0	0.0	0.0
50	0.12678373931105177	0.0	0.0	0.0	0.0
51	0.13217879204769226	0.0	0.0	0.0	0.0
52	0.13757384478433277	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAATCG	15	0.004537935	58.362396	33
GACGGAT	15	0.004537935	58.362396	34
CTACCCG	15	0.004537935	58.362396	34
AACTCCT	15	0.004562511	58.282993	58
CTCGGAA	15	0.004562511	58.282993	54
ATGTAAC	15	0.004562511	58.282993	54
TGTAACT	15	0.004562511	58.282993	55
CAATGGT	15	0.004587186	58.203804	28
TGGTAGT	15	0.004587186	58.203804	31
ACTTGCA	15	0.004587186	58.203804	35
GTATCTA	15	0.004587186	58.203804	28
GGATTCG	15	0.004587186	58.203804	37
GTTTTGC	15	0.004587186	58.203804	28
CGGATTC	15	0.004587186	58.203804	36
TCTAAAT	15	0.004587186	58.203804	31
AAGACGG	15	0.004587186	58.203804	35
AATGGTA	15	0.004587186	58.203804	29
CTAAATC	15	0.004587186	58.203804	32
ATCGTTG	15	0.004587186	58.203804	36
TACCCGT	15	0.004587186	58.203804	35
>>END_MODULE