SRR1526509_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34337
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	213	0.6203221015231383	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	200	0.5824620671578763	No Hit
ACGAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	164	0.47761889506945865	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	125	0.3640387919736727	No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	118	0.343652619623147	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	116	0.33782799895156823	No Hit
ACGAGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	115	0.3349156886157789	No Hit
ACGAGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC	115	0.3349156886157789	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	97	0.28249410257157	No Hit
ACGAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	92	0.26793255089262313	No Hit
ACGAGCGGGCAGGACATATTACTGGATGAATATATTGATGCACATGGTTGTTTCCT	84	0.24463406820630804	No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.2358971371989399	No Hit
ACGAGCGGGTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC	80	0.23298482686315053	No Hit
ACGAGCGGGGGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGC	75	0.21842327518420365	No Hit
ACGAGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCAT	70	0.20386172350525672	No Hit
ACGAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	65	0.18930017182630982	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC	64	0.18638786149052042	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	63	0.18347555115473105	No Hit
ACGAGCGGGCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCC	62	0.18056324081894168	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	61	0.17765093048315228	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	58	0.16891399947578412	No Hit
ACGAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	57	0.16600168913999477	No Hit
ACGAGCGGGGATTGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTT	55	0.160177068468416	No Hit
ACGAGCGGGAGGCCTATATTAAAAACCATAATACCAAAAAGGAAAGTCTGTAGTCC	53	0.1543524477968372	No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGCCAG	49	0.1427032064536797	No Hit
ACGAGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	46	0.13396627544631157	No Hit
ACGAGCGGGGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGATGGTTTTCTTT	45	0.13105396511052217	No Hit
ACGAGCGGGGAGAGTCGTCACCAGCGTCCTTCGGAGCGGAAGGAATATGGCGTCCG	43	0.1252293444389434	No Hit
ACGAGCGGGGGTACTATATCATCTCCCATGAGCCGGTGACTGGACCTGCTTCCTGA	41	0.11940472376736466	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	41	0.11940472376736466	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGAGCACTGATTGCTCTTCCA	41	0.11940472376736466	No Hit
ACGAGCGGGACTGCGTAGCCCAGGCTGGTCTTGGACTTGCAGCCCTCCCCTGCCTC	41	0.11940472376736466	No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG	39	0.1135801030957859	No Hit
ACGAGCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	38	0.11066779275999651	No Hit
ACGAGCGGGATGGATATGGTGATTATAGCAATCAGCAGAGTGGTTATGGGAAAGTA	37	0.10775548242420711	No Hit
ACGAGCGGGGGTGCTTCTGCGCTCATGATCGCGATCACCTGGGCACTGTCCGCGCT	37	0.10775548242420711	No Hit
ACGAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.10193086175262836	No Hit
ACGAGCGGGTCATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCAGCCTCCA	35	0.10193086175262836	No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCC	35	0.10193086175262836	No Hit
ACGAGCGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCT	35	0.10193086175262836	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCAAG	25	1.8772991E-5	50.724705	37
ACTGAAG	15	0.007898137	50.7247	37
AGACTCT	15	0.007898137	50.7247	37
TGCTCAA	20	3.871313E-4	50.64933	36
TCAGACT	15	0.007944802	50.64933	35
CAGACTC	15	0.007944802	50.64933	36
CTGCTCA	20	3.871313E-4	50.64933	35
ATTCCAA	15	0.007944802	50.64933	34
AAGGAAC	15	0.008038748	50.49926	32
CCGGGCA	15	0.008038748	50.49926	33
AGATCAG	15	0.008038748	50.49926	32
CAGATCA	15	0.008086029	50.424557	31
GTTCGCG	15	0.008133518	50.350075	48
TAGCAAG	45	1.2187229E-10	50.275814	47
TGCCTAG	50	5.456968E-12	50.275814	43
CCTAGCA	45	1.2187229E-10	50.275814	45
CTTTCAA	15	0.0081812125	50.27581	47
TTCGCGC	15	0.0081812125	50.27581	49
CAACTGC	15	0.0081812125	50.27581	44
CAGTACA	15	0.0081812125	50.27581	49

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content