FastQCFastQC Report
Fri 17 Jun 2016
SRR1526508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121002
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.9404803226392953No Hit
CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.4561908067635245No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.2809870911224608No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.24297119055883376No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGTGGTATCAA2660.21983107717227812No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2550.21074031834184562No Hit
CGTATTGGGGTATGCTAAGGCCCGCCCCTCACCCCTACTTTGGCCATCCGTAAGATAGCAGGAT2140.1768565808829606No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.1752037156410638No Hit
CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2110.17437728302011538No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1970.16280722632683758No Hit
CGTATTGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGT1790.14793143914976611No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC1730.14297284342407562No Hit
CGTATTGGGTCTTGGAATTTTCTTTTGTTTGCTCCTGCATTTGACTAACTGTAATAAAAAAAAA1670.13801424769838516No Hit
CGTATTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1660.13718781507743674No Hit
CGTATTGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGACGCTAGAGGTAAC1590.13140278673079783No Hit
CGTATTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1510.12479132576321053No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1231384605213137No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1231384605213137No Hit
CGTATTGGGGGTGTGTTTGCCTGGAAGTCCCTCGCCCACAGTGTGTGTTCACACCGACTCCACC1480.1223120279003653No Hit
CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.11404770169088113No Hit
CGTATTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1360.1123948364489843No Hit
CGTATTGGGAGGTTCTATGGGCAGCAAAGAATAGTTGTGTACAGTGAACGGGTATGTGTTTGCC1350.1115684038280359No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG1340.11074197120708748No Hit
CGTATTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.10908910596519066No Hit
CGTATTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1310.10826267334424224No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1310.10826267334424224No Hit
CGTATTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1280.105783375481397No Hit
CGTATTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1240.10247764499760334No Hit
CGTATTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1230.10165121237665493No Hit
CGTATTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1230.10165121237665493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA201.9618975E-458.2443852
ACGCTAG201.9618975E-458.2443851
GACGCTA201.9618975E-458.2443850
GATTAAG201.9618975E-458.2443850
AGATAGC258.410407E-658.24437753
ACACCGA258.410407E-658.24437751
ATAGCAG258.410407E-658.24437755
CGACATT150.004599831558.24437754
ACTTAAC150.004599831558.24437752
TTGCGTC150.004599831558.24437754
AGCTCGA150.004599831558.24437751
AGAGGTA150.004599831558.24437756
TAAGATA258.410407E-658.24437751
GATAGCA258.410407E-658.24437754
CATGCAT150.004607422358.2201458
CTATAAC150.004607422358.2201458
CAGTGCG150.004607422358.2201457
CGTCACA150.004607422358.2201457
ATCGCGA201.9659413E-458.2201458
CGACGCT201.969992E-458.19592749
Produced by FastQC (version 0.11.5)