FastQCFastQC Report
Fri 17 Jun 2016
SRR1526507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117841
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.7722269838171774No Hit
CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.4107229232610043No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGTGGTATCAA2820.23930550487521318No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.20281565838714877No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2280.19348104649485323No Hit
CGTATTGGGGTATGCTAAGGCCCGCCCCTCACCCCTACTTTGGCCATCCGTAAGATAGCAGGAT1990.1688716151424377No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.15529399784455328No Hit
CGTATTGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGGGGACAGT1720.1459593859522577No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.1417163805466688No Hit
CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1620.1374733751410799No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1610.13662477405996215No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.11710694919425328No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC1350.11456114595089993No Hit
CGTATTGGGGGTGTGTTTGCCTGGAAGTCCCTCGCCCACAGTGTGTGTTCACACCGACTCCACC1220.10352933189636884No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.10183212973413328No Hit
CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.1009835286530155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTG201.9680371E-458.2062539
CACGTAC150.0046114658.2062538
ATTTGAC201.9680371E-458.2062539
TAATAAA201.9763834E-458.1564852
TACCCTT201.9763834E-458.1564842
ACGCACC150.00462711358.1564850
CGCATAT150.00462711358.1564847
GCATTAT150.00462711358.1564848
CCGTAAG150.00462711358.1564848
CTAAACG150.00462711358.1564846
CGTAAGA150.00462711358.1564849
ACCGTGT150.00462711358.1564847
GGCGATA150.00462711358.1564858
GTAAACA150.00462711358.1564849
TGACTAA201.9763834E-458.1564842
TGCAACT150.00462711358.1564842
ATGATCG258.484638E-658.15647555
ACACCGA201.9805669E-458.13162651
ATTCGCC150.00463495558.13162651
TATGCAA150.00463495558.13162640
Produced by FastQC (version 0.11.5)