FastQCFastQC Report
Fri 17 Jun 2016
SRR1526506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107600
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12531.1644981412639406No Hit
CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.5827137546468402No Hit
CGTATTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3710.3447955390334572No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.3224907063197026No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGG2840.2639405204460966No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGT2560.23791821561338292No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.20539033457249073No Hit
CGTATTGGGGTATGCTAAGGCCCGCCCCTCACCCCTACTTTGGCCATCCGTAAGAT2150.19981412639405205No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2050.19052044609665428No Hit
CGTATTGGGTCTTGGAATTTTCTTTTGTTTGCTCCTGCATTTGACTAACTGTAATA1840.17100371747211895No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1820.16914498141263942No Hit
CGTATTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1820.16914498141263942No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.16635687732342005No Hit
CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1750.16263940520446096No Hit
CGTATTGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAG1530.14219330855018586No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1440.13382899628252787No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.12732342007434944No Hit
CGTATTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1330.12360594795539034No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA1290.11988847583643122No Hit
CGTATTGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGACGCTA1280.11895910780669146No Hit
CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.1171003717472119No Hit
CGTATTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1260.1171003717472119No Hit
CGTATTGGGGGTGTGTTTGCCTGGAAGTCCCTCGCCCACAGTGTGTGTTCACACCG1260.1171003717472119No Hit
CGTATTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1250.11617100371747212No Hit
CGTATTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1230.11431226765799257No Hit
CGTATTGGGGGAGCTGGCAACCTGAGGAAGCTGTACACGCTCCTGAATAACTGCAT1230.11431226765799257No Hit
CGTATTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1200.11152416356877323No Hit
CGTATTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1140.10594795539033458No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.10594795539033458No Hit
CGTATTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1100.10223048327137546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT150.00795209850.71313537
ACGCACA150.00795209850.71313537
GTCACCG203.8852936E-450.71313537
CAGTACG251.9077757E-550.71313537
TCAGTAC251.9239049E-550.641136
TACGCAC150.00799693650.64109836
GTTAAAA150.00799693650.64109834
GAGGTCT150.00799693650.64109836
CTACGCA150.00799693650.64109835
AAAACTC150.00807208550.52149233
CACTACG150.00807208550.52149233
AGTTAAA150.00807208550.52149233
GACACTA150.00808717750.4976431
ACACTAC150.00808717750.4976432
CACACGA150.00808717750.4976431
ACCCGGA150.00808717750.4976431
CGACTCA251.9565023E-550.4976432
CCAGTTA150.00808717750.4976431
GTGCGAT203.9678742E-450.4976431
TTCACAC150.008223955550.28396648