##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526506_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 107600 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.23065985130111 39.0 38.0 40.0 35.0 40.0 2 37.77231412639405 39.0 38.0 40.0 35.0 40.0 3 37.05736059479554 39.0 36.0 40.0 32.0 40.0 4 37.50017657992565 39.0 38.0 40.0 33.0 40.0 5 37.67881970260223 39.0 38.0 40.0 35.0 40.0 6 38.0474907063197 39.0 38.0 40.0 35.0 40.0 7 37.685975836431226 39.0 38.0 40.0 35.0 40.0 8 38.0471375464684 39.0 38.0 40.0 35.0 40.0 9 38.01709107806691 39.0 38.0 40.0 35.0 40.0 10 37.13633828996282 39.0 37.0 40.0 33.0 40.0 11 37.64031598513011 39.0 38.0 40.0 35.0 40.0 12 37.53208178438662 39.0 38.0 40.0 33.0 40.0 13 37.49992565055762 39.0 38.0 40.0 33.0 40.0 14 37.5142843866171 39.0 38.0 40.0 33.0 40.0 15 37.48691449814127 39.0 38.0 40.0 33.0 40.0 16 37.37871747211896 39.0 38.0 40.0 33.0 40.0 17 37.33279739776952 39.0 38.0 40.0 33.0 40.0 18 37.22369888475836 39.0 38.0 40.0 33.0 40.0 19 37.11390334572491 39.0 37.0 40.0 33.0 40.0 20 37.12509293680297 39.0 37.0 40.0 33.0 40.0 21 36.98648698884758 39.0 37.0 40.0 33.0 40.0 22 36.904702602230486 39.0 37.0 40.0 33.0 40.0 23 36.67782527881041 39.0 36.0 40.0 32.0 40.0 24 36.5557156133829 39.0 36.0 40.0 31.0 40.0 25 36.477156133829 39.0 36.0 40.0 31.0 40.0 26 36.00148698884758 38.0 36.0 40.0 30.0 40.0 27 35.91573420074349 38.0 35.0 40.0 30.0 40.0 28 35.635325278810406 38.0 35.0 40.0 29.0 40.0 29 35.53968401486989 38.0 35.0 40.0 29.0 40.0 30 35.33253717472119 38.0 35.0 40.0 29.0 40.0 31 34.67284386617101 38.0 34.0 39.0 26.0 40.0 32 34.408252788104086 38.0 34.0 39.0 25.0 40.0 33 34.30219330855019 38.0 33.0 39.0 25.0 40.0 34 34.48881970260223 38.0 34.0 39.0 25.0 40.0 35 34.25646840148699 38.0 34.0 39.0 25.0 40.0 36 33.75982342007435 38.0 33.0 39.0 22.0 40.0 37 33.25045539033457 38.0 33.0 39.0 16.0 40.0 38 33.309052044609665 38.0 33.0 39.0 18.0 40.0 39 33.14756505576208 38.0 33.0 39.0 17.0 40.0 40 32.77466542750929 38.0 32.0 39.0 10.0 40.0 41 32.119795539033454 37.0 31.0 39.0 4.0 40.0 42 31.989442379182154 36.0 31.0 39.0 4.0 40.0 43 31.799869888475836 36.0 31.0 39.0 4.0 40.0 44 31.312546468401486 36.0 30.0 39.0 4.0 40.0 45 31.233736059479554 36.0 30.0 39.0 4.0 40.0 46 30.644061338289962 36.0 30.0 39.0 4.0 40.0 47 30.401328996282526 35.0 29.0 39.0 4.0 40.0 48 30.002369888475837 35.0 28.0 39.0 4.0 40.0 49 29.836812267657994 35.0 28.0 39.0 4.0 40.0 50 29.44242565055762 35.0 28.0 39.0 4.0 40.0 51 28.68842936802974 35.0 26.0 39.0 4.0 40.0 52 28.461589219330854 35.0 25.0 39.0 4.0 40.0 53 28.050920074349442 35.0 23.0 39.0 4.0 40.0 54 27.716775092936803 34.0 18.0 38.0 4.0 40.0 55 27.055 34.0 4.0 38.0 4.0 40.0 56 16.018986988847583 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.0741974931601419 1 2 0.16626699459568783 1 3 0.19849692169709243 1 4 0.15086478328669273 1 5 0.4551599316029282 1 6 -0.29307397502527266 1 7 0.25192301657084926 1 8 -0.38653640932902533 1 9 -1.133466672677855 1 10 0.26579356098721263 1 11 -1.0296928428273873 1 12 -0.5937771465943626 1 13 -0.8879481094021529 1 14 -0.8631800120732507 1 15 -1.8805072756407384 1 16 -1.2823339260896347 1 17 -1.1338494419065128 1 18 -1.3142596057870222 1 19 -0.9093685458495102 1 20 -1.8431735896997168 1 21 -0.8775554099041969 1 22 -0.8629028804431655 1 23 -1.6105975191286461 1 24 -1.1052226833893855 1 25 -1.1534173822685219 1 26 -1.3665833488215569 1 27 -1.2454097762993683 1 28 -1.0914763843548982 1 29 -0.23386676839329112 1 30 -1.0543090921954175 1 31 0.2767067103827685 1 32 -0.18511264070059497 1 33 0.12590255132631256 1 34 -0.6594881300562889 1 35 0.5211370530780925 1 36 -0.040133600281393456 1 37 -0.22415491476604643 1 38 -0.38055731405413695 1 39 -0.2753877750601603 1 40 -0.9530590470844871 1 41 -0.17974359548055574 1 42 -0.19102919348809522 1 43 -0.26898932410142606 1 44 -0.09307028123829042 1 45 0.3171213550325014 1 46 0.6803723775664139 1 47 1.0346870836112423 1 48 1.6468221737840274 1 49 1.5738030850291622 1 50 1.9602329427238665 1 51 2.623580701371935 1 52 1.8582574538660772 1 53 1.6839195969961729 1 54 1.5821840224466506 1 55 1.8105829523203134 1 56 2.3291956939730856 2 1 0.5940230284495911 2 2 0.9998316258407343 2 3 1.0935207123394406 2 4 0.48442941453173916 2 5 0.6923724613412361 2 6 0.6248483088763876 2 7 0.8297382425819535 2 8 0.4860845264362368 2 9 0.1971241282737708 2 10 0.6854010153884786 2 11 -0.19216310452445384 2 12 -0.1438366232002295 2 13 -0.2338045725266582 2 14 -0.1520380612405745 2 15 -0.27374676810703136 2 16 -0.18826176114118454 2 17 -2.7078702707683533E-5 2 18 -0.6727052838203278 2 19 -0.2363907504490328 2 20 -0.708756460437229 2 21 -0.5437925233062586 2 22 0.053334232958896166 2 23 -0.16967761429121708 2 24 0.09511435071053853 2 25 0.32100767720807966 2 26 0.14679888749882508 2 27 -0.4106556129369565 2 28 -0.5786889140932061 2 29 -0.3596597898048728 2 30 -0.5818665862477559 2 31 -0.05071200492254491 2 32 -0.8952633147687976 2 33 -0.28289998634220836 2 34 -0.42911540999284625 2 35 -0.2903221063192163 2 36 0.3057228628431119 2 37 0.20933041433784183 2 38 0.9116710761123983 2 39 0.16582951280661007 2 40 1.2917863137402534 2 41 0.06698519119668589 2 42 0.5743355963612267 2 43 0.6208798273656626 2 44 1.069697363488114 2 45 -0.003854061303737666 2 46 -0.20771247572462315 2 47 0.1513603587896739 2 48 -0.24770597847608045 2 49 -0.24290984121984494 2 50 -0.49595262428644205 2 51 -0.9561179504916666 2 52 -0.0016751393139387005 2 53 -0.13646105328138347 2 54 -0.22550828524565603 2 55 -1.0359674045393241 2 56 0.13748276772407664 3 1 -0.049406458729897906 3 2 0.37803675404585846 3 3 -0.032280569711836904 3 4 -0.43944879059646524 3 5 0.08660323057200259 3 6 -0.01697861420053215 3 7 0.3008920887358002 3 8 -0.2567039351022231 3 9 0.019239512889157595 3 10 0.28716383590129624 3 11 -0.08639387375522034 3 12 -0.5637084180720251 3 13 -0.6320417520138406 3 14 -0.5342495997021146 3 15 -0.40595830656857146 3 16 -0.7715950944745202 3 17 -0.6634886171642478 3 18 -0.9715834889485322 3 19 -1.1474484427567262 3 20 -0.579750050180813 3 21 -0.6479591899729229 3 22 -1.3633324337077681 3 23 -0.6207994091630127 3 24 -1.154084367238184 3 25 -0.9217807843303802 3 26 -1.1112139330139925 3 27 -0.4995979206292631 3 28 -0.7854196833239726 3 29 -0.7322559436510261 3 30 -1.1259370990682669 3 31 -1.8824427741533114 3 32 -1.2374107506662284 3 33 -1.1739256273678507 3 34 -1.7472243843518172 3 35 -1.973815696062804 3 36 -1.4418732910030414 3 37 -1.3507657395083115 3 38 -1.2501878982465762 3 39 -1.2692666410395432 3 40 -1.2811303529264144 3 41 -1.057213526752033 3 42 -1.5386451728695398 3 43 -1.2965881213522827 3 44 -1.342962892922138 3 45 -1.2626681638678399 3 46 -2.131590680852831 3 47 -1.984857589928275 3 48 -1.4736675169376205 3 49 -1.3935508668608705 3 50 -1.38136929095311 3 51 -1.2093230786967943 3 52 -1.3077648829036832 3 53 -1.186140540460869 3 54 -1.5219826442200173 3 55 -1.3188199686418862 3 56 -1.575658257916949 4 1 -0.34284199332108756 4 2 0.22316254020937976 4 3 -0.024222393611211146 4 4 -0.10356042581659608 4 5 0.13849002302483626 4 6 0.07912987636550639 4 7 0.2112694472263641 4 8 -0.08630299170599898 4 9 0.16900366383255516 4 10 -0.13480943453895122 4 11 0.08341744699949771 4 12 0.08566265111036842 4 13 0.12994724169685412 4 14 0.24817964558090466 4 15 -0.05041585373838586 4 16 -0.8038277988770304 4 17 -0.6658471077302863 4 18 -0.18286493548941252 4 19 -0.3650191974737069 4 20 -0.8488145155896234 4 21 -0.29615101387229714 4 22 0.008522912204178112 4 23 -0.6878198494145806 4 24 0.08549896609515173 4 25 -0.14524776546245732 4 26 -0.21223594559261016 4 27 -0.3905177319500197 4 28 -1.010655532380575 4 29 -0.3602040568585707 4 30 -0.291227979571417 4 31 -0.8977729628325548 4 32 -0.739965782112769 4 33 -1.0717243695062137 4 34 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0.6174046062895613 5 19 0.41296822390994237 5 20 0.36340471172394473 5 21 0.18596938145564934 5 22 0.054524709149369244 5 23 0.3116410670274661 5 24 0.4140703946665809 5 25 -0.01910221290180658 5 26 0.6893813050812412 5 27 0.3566520793707397 5 28 0.5461279357236464 5 29 0.5924464373013549 5 30 0.6505509961698266 5 31 0.08839055918002003 5 32 0.13074401123853363 5 33 0.0903600869178689 5 34 0.5250970442196063 5 35 0.8651723991752931 5 36 0.145924328044579 5 37 -1.8123728823654552 5 38 0.09445495889627864 5 39 0.5846024065795064 5 40 0.05607202802596589 5 41 0.6097507589622495 5 42 0.22772387474951117 5 43 0.6090666405524772 5 44 0.9251918689826262 5 45 0.5197723123226368 5 46 -0.37117401418616325 5 47 -0.44140520897589397 5 48 -0.9004532312233344 5 49 -1.2554556287656347 5 50 -1.712619290953107 5 51 -0.9700373644110805 5 52 -1.003598216237016 5 53 -0.9998310166513455 5 54 -1.1606731204104932 5 55 -0.13816520673712418 5 56 -0.16672968648837738 6 1 -0.04992213529754963 6 2 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0.21217838029788183 6 44 1.0451352918963401 6 45 0.7986973476833157 6 46 0.9951626194772025 6 47 1.5050351493456517 6 48 1.3524463444485164 6 49 1.7665151397397878 6 50 0.8431562866046463 6 51 0.433828736484724 6 52 0.4498671302976369 6 53 0.22949477307048127 6 54 0.8396097650209065 6 55 0.38177821617659546 6 56 -0.20086452854401138 7 1 -0.09720630296665433 7 2 0.4531925172857356 7 3 -0.3945274544470436 7 4 0.12937909102347334 7 5 0.24781818384303023 7 6 0.1550424138368527 7 7 0.5976210606984154 7 8 0.23365821443048418 7 9 0.3562722231695332 7 10 0.5413499729729523 7 11 0.23603603278683494 7 12 -0.08785172025270782 7 13 0.2833593383288431 7 14 0.42865927880255583 7 15 0.07681038502021664 7 16 -0.45344867702903713 7 17 -0.08288114052873397 7 18 -0.31397056059962836 7 19 0.15190903387878762 7 20 -0.12248758912162572 7 21 -0.3486990653623323 7 22 -0.04596483246166372 7 23 0.29401756903013876 7 24 0.2677419567493544 7 25 -0.315764429190196 7 26 0.5478592757087455 7 27 0.6987081541370941 7 28 0.5177345222835044 7 29 0.8451389161620568 7 30 0.7565590068507362 7 31 1.39617872117379 7 32 1.2093376917013714 7 33 1.3393142480215587 7 34 1.1930988243709209 7 35 0.7281897556505896 7 36 0.4334187650717283 7 37 0.4071197745103774 7 38 0.5899600768313036 7 39 -0.18077365038534055 7 40 1.3174872483196935 7 41 0.8237249467682162 7 42 1.4885160420837344 7 43 1.1281587633829204 7 44 0.4854054871401665 7 45 0.7713076928299785 7 46 1.469947481140938 7 47 1.1913495751807588 7 48 1.8960754737165857 7 49 1.6080067655378834 7 50 1.2867576560873886 7 51 1.937483774885763 7 52 1.6001401015199939 7 53 2.0040853162369494 7 54 0.6008756112316966 7 55 1.8033568226353722 7 56 1.5129320847621788 8 1 0.4597847177406962 8 2 0.8853896952223295 8 3 0.6374498224450207 8 4 0.32096787607019905 8 5 0.9542502893955316 8 6 0.2900066799171128 8 7 0.6979509122652132 8 8 0.5809186139173832 8 9 0.6479159834773895 8 10 1.067188345705219 8 11 0.7077237733036057 8 12 0.46080138584954256 8 13 0.6430807970057728 8 14 0.716363145395924 8 15 0.34710541892162183 8 16 0.4318362780744991 8 17 0.15268785342398417 8 18 0.1417743541887191 8 19 0.31885057685111917 8 20 0.3583626949172256 8 21 0.1988545355172704 8 22 0.23715776237066422 8 23 0.3883917673075814 8 24 0.08549896609515173 8 25 0.3545672548853034 8 26 -0.22518452124928956 8 27 0.2546912950570146 8 28 -0.31605693822593395 8 29 -0.5943882965922 8 30 0.302371724461139 8 31 0.9864297748662949 8 32 0.5609961120788682 8 33 0.9584273138086203 8 34 0.02789816466778916 8 35 0.9777774411920959 8 36 0.9778571011538233 8 37 1.6951901428446305 8 38 1.0157434743024467 8 39 0.4114931628820244 8 40 1.2182569019755505 8 41 1.5965854928558088 8 42 1.0036342388951631 8 43 0.5961814864908561 8 44 1.3279929894308005 8 45 1.6827975224066662 8 46 1.471963240715798 8 47 1.2933286845815282 8 48 2.5373618948270824 8 49 1.871155015492068 8 50 1.6167924737527741 8 51 1.0823435879698735 8 52 0.20816648964533613 8 53 -0.025601324774594758 8 54 0.8959095126427279 8 55 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145.0 12 144.0 13 183.0 14 308.0 15 352.0 16 364.0 17 488.0 18 628.0 19 699.0 20 742.0 21 935.0 22 1033.0 23 964.0 24 1318.0 25 1645.0 26 1558.0 27 1874.0 28 2133.0 29 2339.0 30 2724.0 31 3330.0 32 3809.0 33 4703.0 34 6259.0 35 8261.0 36 11449.0 37 16879.0 38 19137.0 39 12004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.48114742665861 0.2770933097773025 0.17481054442326469 0.06694871914082477 8 99.4460966542751 0.3856877323420074 0.1013011152416357 0.06691449814126393 9 98.02044609665428 1.1440520446096654 0.4024163568773234 0.43308550185873607 10 45.847583643122675 36.726765799256505 6.0241635687732344 11.401486988847584 11 28.868959107806692 31.481412639405203 19.79553903345725 19.854089219330856 12 36.68680297397769 17.861524163568774 24.08364312267658 21.36802973977695 13 24.64033457249071 17.46189591078067 29.059479553903344 28.838289962825282 14 24.57434944237918 24.597583643122675 27.71468401486989 23.113382899628252 15 29.762081784386616 20.435873605947954 27.0910780669145 22.710966542750928 16 26.065055762081784 21.83921933085502 27.010223048327138 25.08550185873606 17 30.827137546468403 21.855947955390334 21.33550185873606 25.981412639405203 18 29.458178438661708 23.86431226765799 22.599442379182154 24.07806691449814 19 25.49814126394052 22.63289962825279 26.438661710037177 25.430297397769518 20 24.678438661710036 26.537174721189594 22.027881040892193 26.756505576208177 21 24.904275092936803 20.857806691449817 28.402416356877325 25.83550185873606 22 26.701672862453528 20.607806691449817 23.52881040892193 29.16171003717472 23 28.041821561338292 25.994423791821564 23.605947955390334 22.357806691449813 24 25.77278388074128 28.18175059015967 22.838714474246732 23.20675105485232 25 26.093626516665893 22.55032309051183 24.375435823532147 26.98061456929013 26 27.733194468983346 21.303899050576998 26.10494797236351 24.857958508076138 27 24.51362591762112 20.030889755207994 25.723164525163057 29.732319802007833 28 23.800217761523214 25.30593632801958 26.878658440120233 24.015187470336972 29 28.5868553342022 21.359150996090115 26.624464717929623 23.429528951778067 30 24.20518076779724 24.57510249720462 28.211889675736117 23.00782705926202 31 26.03757058461768 23.254253713263385 29.005385862576645 21.702789839542294 32 23.864801864801866 21.53006993006993 33.71561771561772 20.889510489510492 33 26.347109904767237 21.747768419285336 31.438938168658066 20.466183507289365 34 23.18591830452506 23.819296101788197 33.457086089008705 19.53769950467804 35 21.702417496291183 27.66545060973903 32.70384504138015 17.928286852589643 36 25.091465691032628 23.711080415142238 32.11099081609796 19.08646307772717 37 22.651642201661986 24.44647534342083 33.98028981137764 18.92159264353954 38 24.62588973788929 23.684308853475816 33.48870663403516 18.201094774599735 39 22.931905869603806 22.632960585932757 33.514568911558904 20.920564632904533 40 22.466176677121858 22.816347549272038 35.128505219793084 19.588970553813024 41 21.05784783970529 23.068076631791534 35.02286050882163 20.851215019681543 42 22.13429862221848 21.950968562636213 34.82616381850324 21.088568996642067 43 21.256445530007394 21.718745613296274 37.068231374640874 19.956577482055458 44 20.797955496475478 23.94240940621402 35.044887336996716 20.214747760313788 45 20.625409605842147 23.93315232656118 34.78513247823238 20.65630558936429 46 20.459292143144804 23.492830584511065 34.97700729585195 21.070869976492183 47 22.202767738431863 23.41903709668358 34.503099192899015 19.875095971985544 48 23.125966902629973 24.165768130889315 33.27363930429891 19.4346256621818 49 21.951128066212377 24.256001199771294 33.12212359518967 20.670747138826663 50 20.4735702434406 25.454869280739416 34.41820789471217 19.653352581107807 51 20.41833789406605 27.082318101735574 33.328335270082846 19.17100873411553 52 21.835006891120297 25.147432471708903 32.98549583251296 20.032064804657836 53 21.0997646308643 25.120732176179892 32.433116718710444 21.346386474245367 54 22.259323178509792 24.951226832745554 31.749455991596008 21.039993997148645 55 22.20940267146931 25.982102656461052 30.951710941017556 20.85678373105208 56 22.270480192865115 25.17846589683217 31.79366434340497 20.75738956689774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 6.0 5 9.0 6 17.0 7 25.0 8 161.5 9 298.0 10 298.0 11 298.0 12 283.0 13 248.5 14 229.0 15 415.5 16 602.0 17 849.5 18 1097.0 19 1136.5 20 1176.0 21 1473.0 22 2083.5 23 2397.0 24 2874.0 25 3351.0 26 3449.0 27 3547.0 28 3968.0 29 4370.5 30 4352.0 31 3517.5 32 2683.0 33 2864.0 34 3045.0 35 2435.5 36 1826.0 37 1648.5 38 1591.5 39 1712.0 40 1779.5 41 1847.0 42 1963.5 43 2080.0 44 2253.5 45 2427.0 46 2471.5 47 2610.5 48 2705.0 49 3080.5 50 3456.0 51 4176.0 52 4896.0 53 5184.0 54 5277.0 55 5082.0 56 6017.5 57 6953.0 58 7290.5 59 7628.0 60 6958.5 61 6289.0 62 5870.0 63 4900.5 64 4350.0 65 4036.5 66 3723.0 67 3109.5 68 2496.0 69 2150.5 70 1805.0 71 1563.5 72 1246.0 73 1170.0 74 879.5 75 589.0 76 469.0 77 349.0 78 297.0 79 174.5 80 104.0 81 77.0 82 50.0 83 38.5 84 27.0 85 18.5 86 10.0 87 8.0 88 4.0 89 2.0 90 2.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05111524163568773 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.001858736059479554 25 0.041821561338289966 26 0.056691449814126396 27 0.11245353159851301 28 0.13289962825278812 29 0.16728624535315986 30 0.26022304832713755 31 0.2620817843866171 32 0.3252788104089219 33 0.36152416356877326 34 0.36895910780669144 35 0.39312267657992567 36 0.4237918215613383 37 1.358736059479554 38 0.5074349442379182 39 0.5176579925650557 40 0.7388475836431226 41 0.6013011152416357 42 0.6403345724907064 43 0.6905204460966543 44 0.7221189591078067 45 0.7342007434944238 46 0.7685873605947955 47 0.741635687732342 48 0.8782527881040891 49 0.8485130111524164 50 0.8559479553903345 51 0.828996282527881 52 0.8745353159851301 53 0.891263940520446 54 0.9144981412639406 55 0.9219330855018587 56 0.9265799256505576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 107600.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.965613382899626 #Duplication Level Percentage of deduplicated Percentage of total 1 72.11417233325756 32.42657992565056 2 11.708657999710642 10.529739776951672 3 5.264245706136453 7.101301115241636 4 2.9865861976313997 5.371747211895911 5 1.951098526341897 4.386617100371748 6 1.297976562015584 3.5018587360594795 7 0.9528140049190831 2.9990706319702602 8 0.6965256391707831 2.5055762081784385 9 0.48777463158547424 1.9739776951672863 >10 2.3251968666680445 17.907063197026023 >50 0.13847839117045244 4.377323420074349 >100 0.07233945807411694 5.171933085501858 >500 0.002066841659260484 0.5827137546468402 >1k 0.002066841659260484 1.1644981412639406 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1253 1.1644981412639406 No Hit CGTATTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 627 0.5827137546468402 No Hit CGTATTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 371 0.3447955390334572 No Hit CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 0.3224907063197026 No Hit CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGG 284 0.2639405204460966 No Hit CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGT 256 0.23791821561338292 No Hit CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.20539033457249073 No Hit CGTATTGGGGTATGCTAAGGCCCGCCCCTCACCCCTACTTTGGCCATCCGTAAGAT 215 0.19981412639405205 No Hit CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 205 0.19052044609665428 No Hit CGTATTGGGTCTTGGAATTTTCTTTTGTTTGCTCCTGCATTTGACTAACTGTAATA 184 0.17100371747211895 No Hit CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 182 0.16914498141263942 No Hit CGTATTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 182 0.16914498141263942 No Hit CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179 0.16635687732342005 No Hit CGTATTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 175 0.16263940520446096 No Hit CGTATTGGGACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAG 153 0.14219330855018586 No Hit CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 144 0.13382899628252787 No Hit CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.12732342007434944 No Hit CGTATTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 133 0.12360594795539034 No Hit CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 129 0.11988847583643122 No Hit CGTATTGGGACTGTAGCGTTCTGGCTGCTTAACAGCTGGCTCTGGTTTCGACGCTA 128 0.11895910780669146 No Hit CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.1171003717472119 No Hit CGTATTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 126 0.1171003717472119 No Hit CGTATTGGGGGTGTGTTTGCCTGGAAGTCCCTCGCCCACAGTGTGTGTTCACACCG 126 0.1171003717472119 No Hit CGTATTGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT 125 0.11617100371747212 No Hit CGTATTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 123 0.11431226765799257 No Hit CGTATTGGGGGAGCTGGCAACCTGAGGAAGCTGTACACGCTCCTGAATAACTGCAT 123 0.11431226765799257 No Hit CGTATTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 120 0.11152416356877323 No Hit CGTATTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 114 0.10594795539033458 No Hit CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.10594795539033458 No Hit CGTATTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 110 0.10223048327137546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.293680297397769E-4 0.0 0.0 0.0 0.0 8 0.006505576208178439 0.0 0.0 0.0 0.0 9 0.007434944237918215 0.0 0.0 0.0 0.0 10 0.020446096654275093 0.0 0.0 0.0 0.0 11 0.03531598513011153 0.0 0.0 0.0 0.0 12 0.0362453531598513 0.0 0.0 0.0 0.0 13 0.03903345724907063 0.0 0.0 0.0 0.0 14 0.04925650557620818 0.0 0.0 0.0 0.0 15 0.05018587360594796 0.0 0.0 0.0 0.0 16 0.05111524163568773 0.0 0.0 0.0 0.0 17 0.055762081784386616 0.0 0.0 0.0 0.0 18 0.062267657992565055 0.0 0.0 0.0 0.0 19 0.0687732342007435 0.0 0.0 0.0 0.0 20 0.09479553903345725 0.0 0.0 0.0 0.0 21 0.10223048327137546 0.0 0.0 0.0 0.0 22 0.12360594795539033 0.0 0.0 0.0 0.0 23 0.13568773234200743 0.0 0.0 0.0 0.0 24 0.13847583643122677 0.0 0.0 0.0 0.0 25 0.14684014869888476 0.0 0.0 0.0 0.0 26 0.15055762081784388 0.0 0.0 0.0 0.0 27 0.15985130111524162 0.0 0.0 0.0 0.0 28 0.1821561338289963 0.0 0.0 0.0 0.0 29 0.18866171003717472 0.0 0.0 0.0 0.0 30 0.20353159851301114 0.0 0.0 0.0 0.0 31 0.2053903345724907 0.0 0.0 0.0 0.0 32 0.2100371747211896 0.0 0.0 0.0 0.0 33 0.2137546468401487 0.0 0.0 0.0 0.0 34 0.22397769516728624 0.0 0.0 0.0 0.0 35 0.2537174721189591 0.0 0.0 0.0 0.0 36 0.2611524163568773 0.0 0.0 0.0 0.0 37 0.266728624535316 0.0 0.0 0.0 0.0 38 0.2732342007434944 0.0 0.0 0.0 0.0 39 0.27973977695167285 0.0 0.0 0.0 0.0 40 0.2853159851301115 0.0 0.0 0.0 0.0 41 0.2936802973977695 0.0 0.0 0.0 0.0 42 0.3020446096654275 0.0 0.0 0.0 0.0 43 0.3066914498141264 0.0 0.0 0.0 0.0 44 0.31691449814126393 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACAT 15 0.007952098 50.713135 37 ACGCACA 15 0.007952098 50.713135 37 GTCACCG 20 3.8852936E-4 50.713135 37 CAGTACG 25 1.9077757E-5 50.713135 37 TCAGTAC 25 1.9239049E-5 50.6411 36 TACGCAC 15 0.007996936 50.641098 36 GTTAAAA 15 0.007996936 50.641098 34 GAGGTCT 15 0.007996936 50.641098 36 CTACGCA 15 0.007996936 50.641098 35 AAAACTC 15 0.008072085 50.521492 33 CACTACG 15 0.008072085 50.521492 33 AGTTAAA 15 0.008072085 50.521492 33 GACACTA 15 0.008087177 50.49764 31 ACACTAC 15 0.008087177 50.49764 32 CACACGA 15 0.008087177 50.49764 31 ACCCGGA 15 0.008087177 50.49764 31 CGACTCA 25 1.9565023E-5 50.49764 32 CCAGTTA 15 0.008087177 50.49764 31 GTGCGAT 20 3.9678742E-4 50.49764 31 TTCACAC 15 0.0082239555 50.283966 48 >>END_MODULE