FastQCFastQC Report
Fri 17 Jun 2016
SRR1526505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78129
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6720.8601159620627423No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.7014040881106887No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3420.43773758783550276No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2660.3404625683165022No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.3340628959797258No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2490.31870368237146257No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2060.2636665002751859No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG1920.24574741773221212No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1880.24062767986279102No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1780.2278283351892383No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1750.2239885317871725No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.1958299735053565No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1430.18303062883180382No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1310.16767141522354057No Hit
GAACGCGGGGTTTGTTTTGTTTTATTTTAGGTTAGGGATCGGCGGGATACAAGGATGGACCCTA1270.16255167735411946No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1250.15999180841940894No Hit
GAACGCGGGTAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGATTTCATTTGTAA1210.15487207054998783No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1140.14591252927850096No Hit
GAACGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1120.14335266034379038No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1070.13695298800701405No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCA1040.13311318460494823No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1000.12799344673552715No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1000.12799344673552715No Hit
GAACGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG970.12415364333346134No Hit
GAACGCGGGATGGTATGTTACAATTAGGGTTTCCTTGCCTCTTTACAGTACACTAATCCGCCTA940.12031383993139551No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC940.12031383993139551No Hit
GAACGCGGGGCTCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACGATGGTCT920.11775397099668497No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGCACCCGACTGTTCTTCTGAAGGTCC900.11519410206197443No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG880.1126342331272639No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG880.1126342331272639No Hit
GAACGCGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCAT860.11007436419255336No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC860.11007436419255336No Hit
GAACGCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGCAGGCAC790.10111482292106645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAA258.4423755E-658.174558
CAACCAA351.576882E-858.174549
ATACAAG258.4423755E-658.174547
TCCGGCA150.004615601658.17449654
TTGATCT150.004615601658.17449647
GTGCACC150.004615601658.17449649
ACCGGCA150.004615601658.17449653
TACTTTA150.004615601658.17449651
TTAATGA150.004615601658.17449658
TTCAGGA150.004615601658.17449654
TCATTTG150.004615601658.17449655
ACTCTGT201.969208E-458.17449648
ATTAATG150.004615601658.17449657
GTAGGCC150.004615601658.17449655
GCTTGAT150.004615601658.17449647
AGTTGAG150.004615601658.17449657
CTTGATT150.004615601658.17449648
GTACTAC150.004615601658.17449647
ATGAATG150.004615601658.17449649
CCAAAGA150.004615601658.17449655
Produced by FastQC (version 0.11.5)