FastQCFastQC Report
Fri 17 Jun 2016
SRR1526504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76709
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5910.7704441460584808No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.557952782593959No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2970.38717751502431264No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2650.34546141912943723No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.28940541527069835No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1840.23986755139553378No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1710.22292038743824064No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1580.20597322348094746No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1460.1903296875203692No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGG1410.1838115475367949No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.17598977955650574No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1330.17338252356307604No Hit
GAACGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1220.15904261559921262No Hit
GAACGCGGGGTTTGTTTTGTTTTATTTTAGGTTAGGGATCGGCGGGATACAAGGATGGACCCTA1220.15904261559921262No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1220.15904261559921262No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1170.1525244756156383No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1060.1381845676517749No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG960.1251482876846263No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA930.12123740369448174No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT930.12123740369448174No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGCACCCGACTGTTCTTCTGAAGGTCC890.1160228917076223No Hit
GAACGCGGGTAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGATTTCATTTGTAA880.11471926371090745No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCA810.10559386773390345No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT800.1042902397371886No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC800.1042902397371886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG351.5572368E-858.264334
ATCGTAG201.9539932E-458.264338
ACGGATC201.9539932E-458.264334
GACGGAT258.363895E-658.264333
TCGTAGT201.9539932E-458.264339
GTTTTTA150.004587139558.264334
GATCGTA201.96036E-458.2260237
CGGATCG201.9667431E-458.1877935
ATATGGC150.004611088458.18778635
TAATACT201.979559E-458.11147751
TTGTAAT201.979559E-458.11147748
ATACTAT201.979559E-458.11147753
GTAATAC201.979559E-458.11147750
AATACTA201.979559E-458.11147752
AACCGTC150.00463513158.11147355
GCGTTAT150.00463513158.11147353
TTATCCA150.00463513158.11147356
CGTTATC150.00463513158.11147354
TGATTTC150.00463513158.11147350
CACTAAT150.00463513158.11147351
Produced by FastQC (version 0.11.5)