SRR1526503_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69014
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	721	1.0447155649578346	No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	569	0.8244703973106906	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	450	0.6520416147448344	No Hit
GAACGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	316	0.45787811168748366	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	290	0.42020459616889333	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	270	0.3912249688469006	No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	197	0.2854493291216275	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	174	0.25212275770133596	No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	173	0.25067377633523635	No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	161	0.23328599994204077	No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	156	0.22604109311154258	No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	151	0.2187961862810444	No Hit
GAACGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGA	151	0.2187961862810444	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	145	0.21010229808444666	No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	130	0.18836757759295217	No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	127	0.18402063349465325	No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	113	0.16373489436925842	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	111	0.16083693163705914	No Hit
GAACGCGGGTAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGATTTC	103	0.1492450807082621	No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	90	0.13040832294896687	No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	87	0.12606137885066798	No Hit
GAACGCGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTGTG	84	0.1217144347523691	No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	83	0.12026545338626946	No Hit
GAACGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	80	0.11591850928797055	No Hit
GAACGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77	0.11157156518967166	No Hit
GAACGCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA	76	0.11012258382357204	No Hit
GAACGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	75	0.1086736024574724	No Hit
GAACGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75	0.1086736024574724	No Hit
GAACGCGGGATGGTATGTTACAATTAGGGTTTCCTTGCCTCTTTACAGTACACTAA	74	0.10722462109137276	No Hit
GAACGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	72	0.1043266583591735	No Hit
GAACGCGGGGTTTGTTTTGTTTTATTTTAGGTTAGGGATCGGCGGGATACAAGGAT	71	0.10287767699307386	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTTCA	15	0.007963491	50.675037	37
AATCGGC	15	0.007963491	50.675037	37
AAATCGG	15	0.00798679	50.637638	36
TAGACAG	15	0.00801014	50.600296	34
AATATGG	15	0.00801014	50.600296	34
TAGGGCG	20	3.918224E-4	50.600296	34
AGTCAAG	20	3.975672E-4	50.451473	33
GTAGGGC	20	3.975672E-4	50.451473	33
ATAGACA	15	0.008104048	50.45147	33
TAGGGAT	15	0.008104048	50.45147	33
TTAGGGA	15	0.008127652	50.4144	32
GGAATAT	15	0.008127652	50.4144	32
TATAGAC	15	0.008127652	50.4144	32
CAGTCAA	20	3.990138E-4	50.4144	32
ACAGTCA	20	4.0046457E-4	50.377388	31
GTTAGGG	15	0.008151308	50.377388	31
GTATAGA	15	0.008151308	50.377388	31
ATCGGCT	15	0.008222581	50.266666	38
ATCGGCG	15	0.008222581	50.266666	38
TTGATCA	25	2.0114037E-5	50.22987	50

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content