FastQCFastQC Report
Fri 17 Jun 2016
SRR1526502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74101
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.8866277108271142No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.43724106287364545No Hit
TCAAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3110.419697439980567No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.4021538170874887No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2500.33737736332843016No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.29419306082239105No Hit
TCAAAGGGGAGATCTGGGCCCCTCACTCGGTCCTTATACTCAGTCATGGCCACAGGACCCCGTT1720.2321156259699599No Hit
TCAAAGGGGCAGCCCTGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCCTAGG1650.22266905979676388No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1560.2105234747169404No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1550.20917396526362667No Hit
TCAAAGGGGCTTCATGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGT1520.2051254369036855No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1390.18758181401060714No Hit
TCAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.18083426674403855No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1230.1659896627575876No Hit
TCAAAGGGGTCAGTGTTCAAGATGAAAATGAATACTTGTAAAAACTAGTTTAAAAAGTTCCCTA1190.16059162494433274No Hit
TCAAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1190.16059162494433274No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1160.15654309658439158No Hit
TCAAAGAGGGGAGTGGCACCGTGGATCCCCAGGAGCTACAGAAGGCCCTGACGACTATGGGATT1070.1443975115045681No Hit
TCAAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG1050.14169849259794065No Hit
TCAAAGGGGAGTGCGCAGTACTGGCCTTCAGCGTGCGCAGAAACTCCGTCTCAGTTGCTATATT1050.14169849259794065No Hit
TCAAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1040.14034898314462693No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.1389994736913132No Hit
TCAAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1030.1389994736913132No Hit
TCAAAGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT1010.13630045478468578No Hit
TCAAAGGGGGGGTTTTTTGTTTTTTTGTTTTTTTGCATTTTCCCTTCAGACAAAACTTTTCCAG1000.13495094533137203No Hit
TCAAAGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1000.13495094533137203No Hit
TCAAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG990.13360143587805834No Hit
TCAAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.1322519264247446No Hit
TCAAAGGGGGTTTTTTTTAACCAATCCTGACCCCTGACCCCTGACCTTCAGAAGTGGATGTAAA970.13090241697143087No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC960.12955290751811718No Hit
TCAAAGGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCTAAA930.125504379158176No Hit
TCAAAGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG860.11605781298497996No Hit
TCAAAGGGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCT850.11470830353166625No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG840.11335879407835252No Hit
TCAAAGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.11065977517172507No Hit
TCAAAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA780.1052617373584702No Hit
TCAAAGGGGTGAGAATCAAGGTCTGAGTTCTGGTCATCCTTGTCCCTTGTGGAACGTGTGGTGG770.10391222790515647No Hit
TCAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.10256271845184275No Hit
TCAAAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG760.10256271845184275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCT351.5812475E-858.14711855
CTTGTTA150.004623292558.14711445
GTCATTC150.004623292558.14711454
GTCATTA303.6488746E-758.14711455
GTTTGAT600.058.14711448
TAGAGTC150.004623292558.14711450
TTAGGAG150.004623292558.14711446
TCATTAC303.6488746E-758.14711456
AGCTTGG406.87578E-1058.14711452
CAACCTA150.004623292558.14711446
TAAAAGA201.9731418E-458.14711456
TGCGGGC150.004623292558.14711456
TTATATG150.004623292558.14711446
CATTACC303.6488746E-758.14711457
AACGGTA150.004623292558.14711449
TAACCGA150.004623292558.14711454
CTACGTG150.004623292558.14711456
CGAATGG150.004623292558.14711446
GAACGGT150.004623292558.14711448
AGTTCTT303.6488746E-758.14711447
Produced by FastQC (version 0.11.5)