FastQCFastQC Report
Fri 17 Jun 2016
SRR1526501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71526
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.7298045465984397No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.3551156222911948No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.33134804127170536No Hit
TCAAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2090.2922014372396052No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2020.2824147862315801No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.26423957721667646No Hit
TCAAAGGGGCAGCCCTGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCCTAGG1620.22649106618572268No Hit
TCAAAGGGGCTTCATGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAACATCTTGT1420.19852920616279393No Hit
TCAAAGGGGAGATCTGGGCCCCTCACTCGGTCCTTATACTCAGTCATGGCCACAGGACCCCGTT1320.18454827615132957No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1270.1775578111455974No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1190.1663730671364259No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1180.16497497413527948No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1170.16357688113413305No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1110.15518832312725442No Hit
TCAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.1426054861169365No Hit
TCAAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC970.13561502111120433No Hit
TCAAAGGGGTCAGTGTTCAAGATGAAAATGAATACTTGTAAAAACTAGTTTAAAAAGTTCCCTA960.1342169281100579No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG950.13281883510891146No Hit
TCAAAGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG950.13281883510891146No Hit
TCAAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG950.13281883510891146No Hit
TCAAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG940.13142074210776503No Hit
TCAAAGGGGGTTTTTTTTAACCAATCCTGACCCCTGACCCCTGACCTTCAGAAGTGGATGTAAA890.12443027710203282No Hit
TCAAAGAGGGGAGTGGCACCGTGGATCCCCAGGAGCTACAGAAGGCCCTGACGACTATGGGATT880.12303218410088639No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.12163409109973995No Hit
TCAAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG830.1160417190951542No Hit
TCAAAGGGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCT800.1118474400917149No Hit
TCAAAGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTCCCTCAGAT790.11044934709056847No Hit
TCAAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.10625506808712916No Hit
TCAAAGGGGGGGTTTTTTGTTTTTTTGTTTTTTTGCATTTTCCCTTCAGACAAAACTTTTCCAG740.10345888208483629No Hit
TCAAAGGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCTAAA740.10345888208483629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG201.957388E-458.2383736
TATGGAA150.00459394958.23836536
ATACTCA303.607547E-758.23836536
TAGCTCG201.9642303E-458.19732737
GTACGTA201.9642303E-458.19732739
GGCTAGC201.9642303E-458.19732734
TCTACGT303.6252095E-758.19732338
CTTATAC303.6252095E-758.19732333
AATTGTG150.004606801658.19732333
CTACGTA303.6252095E-758.19732339
AATTCTG150.004606801658.19732338
GCTAGGC303.6252095E-758.19732339
CTCTACG303.6252095E-758.19732337
TTCGACG150.004606801658.19732358
ATTCTGG150.004606801658.19732339
CTCAGAT150.004606801658.19732358
TGTTTCG150.004619681358.1563455
TCTTGTT150.004619681358.1563455
GTTTCGA150.004619681358.1563456
CTCTTGT150.004619681358.1563454
Produced by FastQC (version 0.11.5)