SRR1526500_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526500_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66389
Sequences flagged as poor quality	0
Sequence length	56
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCAAAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	599	0.9022579041708717	No Hit
TCAAAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	315	0.47447619334528307	No Hit
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	305	0.4594134570486075	No Hit
TCAAAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	246	0.37054331289822107	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	237	0.356986850231213	No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	232	0.3494554820828752	No Hit
TCAAAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	206	0.31029236771151847	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	184	0.27715434785883203	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	160	0.24100378074681048	No Hit
TCAAAGGGGCAGCCCTGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTT	128	0.19280302459744836	No Hit
TCAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.1912967509677808	No Hit
TCAAAGGGGCTTCATGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTGGGGAAAA	122	0.183765382819443	No Hit
TCAAAGGGGAGATCTGGGCCCCTCACTCGGTCCTTATACTCAGTCATGGCCACAGG	116	0.1747277410414376	No Hit
TCAAAGGGGGGGTGTACTGCTCGGAGACTGGGAGCAAGGGGTCCTATACAAGGAAC	110	0.1656900992634322	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	108	0.16267755200409706	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	105	0.15815873111509438	No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104	0.1566524574854268	No Hit
TCAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	103	0.15514618385575923	No Hit
TCAAAGGGGTCAGTGTTCAAGATGAAAATGAATACTTGTAAAAACTAGTTTAAAAA	101	0.1521336365964241	No Hit
TCAAAGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	100	0.15062736296675655	No Hit
TCAAAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	94	0.14158972118875113	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	94	0.14158972118875113	No Hit
TCAAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.14008344755908358	No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	93	0.14008344755908358	No Hit
TCAAAGGGGAACCTAAACAGGTTGATAGGTCAAATTGTGTCTTCCATCACTGCTTC	93	0.14008344755908358	No Hit
TCAAAGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	90	0.13556462667008087	No Hit
TCAAAGAGGGGAGTGGCACCGTGGATCCCCAGGAGCTACAGAAGGCCCTGACGACT	86	0.12953953215141062	No Hit
TCAAAGGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC	83	0.12502071126240794	No Hit
TCAAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	82	0.12351443763274035	No Hit
TCAAAGGGGAGTGCGCAGTACTGGCCTTCAGCGTGCGCAGAAACTCCGTCTCAGTT	82	0.12351443763274035	No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	81	0.1220081640030728	No Hit
TCAAAGGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACT	79	0.11899561674373767	No Hit
TCAAAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	79	0.11899561674373767	No Hit
TCAAAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	78	0.11748934311407011	No Hit
TCAAAGGGGGGGTTTTTTGTTTTTTTGTTTTTTTGCATTTTCCCTTCAGACAAAAC	77	0.11598306948440254	No Hit
TCAAAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.10694542770639714	No Hit
TCAAAGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	71	0.10694542770639714	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGAGCG	15	0.007975443	50.65361	37
TATACTC	15	0.007999712	50.614735	35
TTATACT	15	0.007999712	50.614735	34
AAAGGCT	25	1.921615E-5	50.614735	35
AAGGCTA	30	9.4913594E-7	50.614735	36
ATTGAGC	15	0.007999712	50.614735	36
CTTGCGG	15	0.007999712	50.614735	34
ATACTCA	15	0.007999712	50.614735	36
ATAGCCC	15	0.008024035	50.575924	33
GCTTGCG	15	0.008024035	50.575924	33
GCGAGAT	20	3.9413344E-4	50.537167	32
CGACATT	15	0.008048412	50.537167	32
GTTAAAG	25	1.939163E-5	50.537163	32
TCGACAT	15	0.008072846	50.49847	31
CAGGCGC	15	0.008072846	50.49847	31
CAAATTG	15	0.008072846	50.49847	31
GGCGAGA	20	3.9562822E-4	50.498466	31
CCAGGGC	15	0.008171131	50.344276	50
AATTCTC	20	4.0165242E-4	50.344273	50
TGCATAC	20	4.031698E-4	50.305874	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content