FastQCFastQC Report
Fri 17 Jun 2016
SRR1526497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291478
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC56451.9366813275787538No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC39891.3685423942801858No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC21700.7444815732233651No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT17870.6130822909447711No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC15130.5190786268603462No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC13840.47482142734614613No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13470.4621275019040888No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC12230.4195856977198965No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT10000.3430790660015507No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7780.26691551334920643No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC5820.1996720164129025No Hit
AACGCCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC5270.18080266778281723No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA5040.17291184926478156No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC4890.1677656632747583No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC4730.1622763982187335No Hit
AACGCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4560.15644405409670714No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCATTAGTTGGTCTTCCA4430.15198402623868698No Hit
AACGCCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT4160.1427208914566451No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT4150.14237781239064357No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA4110.14100549612663735No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3900.13380083574060478No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC3760.12899772881658306No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3690.1265961753545722No Hit
AACGCCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC3670.12591001722256911No Hit
AACGCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.12350846376055825No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG3580.12282230562855516No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCA3470.1190484359025381No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC2990.10258064073446367No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2950.10120832447045745No Hit
AACGCCGGGGCTGAAGAGTTGGCTCAGAGGTTAAGAACATTGACTGCTCTTCCGGA2940.10086524540445592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTG150.00804390450.58838334
TGCACGT203.944232E-450.58837534
GACGATT150.00811628650.4738933
ATACCTG150.00813305850.4475432
TATGCGT203.9988838E-450.44753632
CGGTATG203.9988838E-450.44753632
TCGTTAA150.00814985650.4212231
CCGGTAT204.0091976E-450.4212231
CACGGTA150.00826816550.2377448
TATATCC204.0819877E-450.2377448
CGGTACT150.00827383150.2290450
GTCATAT150.008279550.22033747
CTAATGA204.0889744E-450.22033749
TCGTACT150.0082851750.2116445
ATATTAC150.0082851750.2116444
CTCCCGT204.0959707E-450.2029439
CTATAAT204.0994724E-450.1942543
GTCGCAT150.00830788450.1768842
GTCGTTA150.00846241749.943530
AATCCGG204.2163703E-449.90910728
Produced by FastQC (version 0.11.5)