FastQCFastQC Report
Fri 17 Jun 2016
SRR1526496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124274
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.564880827848142No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.4216489370262484No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.31623670276968635No Hit
AAAACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3790.3049712731544812No Hit
AAAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2960.23818336900719378No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2800.22530859230410222No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.19151230345848688No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2330.1874889357387708No Hit
AAAACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2270.18266089447511147No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1960.15771601461287157No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1920.15449732043709868No Hit
AAAACGGGGAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCCAACTCTTTCC1900.15288797334921223No Hit
AAAACGGGGGGTGGGGAGCTAAGGCAAGAGGATCAGGGGGTTGAGGCCAGCTTGGGCTACATAG1770.14242721727795035No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1710.137599176014291No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1700.13679450247034777No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACGCGACTGCTCTTCTGAAGGTC1610.1295524405748588No Hit
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1550.12472439931119944No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1480.11909168450359689No Hit
AAAACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.11748233741571043No Hit
AAAACGGGGGGTCTGCAAAGCTTGTTTTAGGTGCTTGAAAAGGTTAAAAATGAGTTGGTTTCTT1450.11667766387176723No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCAT1410.11345896969599434No Hit
AAAACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1410.11345896969599434No Hit
AAAACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAATA1390.11184962260810788No Hit
AAAACGGGGTCCTCAGAGGGTCTGGAAACACACGGAGACTATAGAACCAGGCCCTTTCCCCCTT1310.1054122342565621No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC1310.1054122342565621No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1270.10219354008078922No Hit
AAAACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1260.101388866536846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATTA201.9685074E-458.20607858
TTGTATT201.9685074E-458.20607857
TGATTAC258.444673E-658.20607853
AACCCGC150.004612136658.2060757
TTATGAA150.004612136658.2060753
TGAACTT150.004612136658.2060756
ATCGTAC150.004612136658.2060756
TATGAAC150.004612136658.2060754
ATACGTT150.004612136658.2060754
AAATACG150.00461953858.18251852
ATTCCCG201.972453E-458.18251852
ATAATGG201.976405E-458.1589849
TATTCCC201.976405E-458.1589851
CGACTAA201.976405E-458.1589848
GACTAAC201.976405E-458.1589849
AGGTAGG150.00462694858.15897850
TTTACGG150.00462694858.15897850
ATTCCAC150.00462694858.15897851
AATGAGT150.00462694858.15897849
ACTGATA201.9803632E-458.13546845
Produced by FastQC (version 0.11.5)