FastQCFastQC Report
Fri 17 Jun 2016
SRR1526495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120798
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.4213645921290088No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.29139555290650504No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.24338151293895596No Hit
AAAACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2830.23427540191062768No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2270.18791701849368367No Hit
AAAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT2070.17136045298763225No Hit
AAAACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1800.14900908955446282No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1740.1440421199026474No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1710.1415586350767397No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1580.13079686749780628No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.1266577261212934No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1520.12582989784599083No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1440.11920727164357026No Hit
AAAACGGGGAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCCAACTCTTTCC1350.11175681716584711No Hit
AAAACGGGGGGTGGGGAGCTAAGGCAAGAGGATCAGGGGGTTGAGGCCAGCTTGGGCTACATAG1350.11175681716584711No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1290.10678984751403169No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACGCGACTGCTCTTCTGAAGGTC1270.10513419096342654No Hit
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1250.10347853441282141No Hit
AAAACGGGGGGTCTGCAAAGCTTGTTTTAGGTGCTTGAAAAGGTTAAAAATGAGTTGGTTTCTT1240.10265070613751884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA150.004602909658.23446337
CGACATT258.418794E-658.23445537
TCGTTAT150.004610521358.2101838
AAATCGC150.004610521358.2101838
TAGCATC150.004610521358.2101838
CGTAGCA150.004610521358.2101836
CATCGTT150.004610521358.2101836
ACCCGTT201.9675895E-458.21017536
AGCGTAG150.004618142358.18591334
CGTGAGG201.971652E-458.1859133
GTTGAGG201.9879683E-458.08905840
TCGCACA150.0046487258.08905846
TAGAATA150.0046487258.08905827
CGCATCG150.0046487258.08905842
ACACACA150.0046487258.08905846
ATATGGT150.0046487258.08905848
TTATCCG150.0046487258.08905841
AAAGTTA150.0046487258.08905848
GTAATAA150.0046487258.08905840
CTTATAT150.0046487258.08905845
Produced by FastQC (version 0.11.5)