FastQCFastQC Report
Fri 17 Jun 2016
SRR1526494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109348
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6510.5953469656509492No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.36214654131762813No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.3520869151699162No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3320.30361780736730437No Hit
AAAACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2940.2688663715842997No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.21948275231371403No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2260.20667959176208067No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2190.20027801148626403No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2150.1966199656143688No Hit
AAAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2100.19204740827449976No Hit
AAAACGGGGTCCTCAGAGGGTCTGGAAACACACGGAGACTATAGAACCAGGCCCTT1950.17832973625489262No Hit
AAAACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1620.14815085781175696No Hit
AAAACGGGGAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCCAA1530.1399202545999927No Hit
AAAACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1520.13900574313201886No Hit
AAAACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1460.13351867432417602No Hit
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1440.1316896513882284No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1370.12528807111241175No Hit
AAAACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1340.12254453670849032No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG1260.11522844496469986No Hit
AAAACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1230.11248491056077843No Hit
AAAACGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1210.11065588762483082No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1210.11065588762483082No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1190.10882686468888321No Hit
AAAACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.10791235322090938No Hit
AAAACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC1180.10791235322090938No Hit
AAAACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACGCGACTGCTCTTC1160.10608333028496178No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTATTCC1130.10333979588104034No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1120.10242528441306653No Hit
AAAACGGGGGGTGGGGAGCTAAGGCAAGAGGATCAGGGGGTTGAGGCCAGCTTGGG1120.10242528441306653No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC1120.10242528441306653No Hit
AAAACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTG1110.10151077294509273No Hit
AAAACGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA1100.10059626147711892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG150.00797387350.6786437
TTAGATC150.00797387350.6786437
GACTATA203.8986545E-450.67863537
TTCTTAG150.00800333650.6314134
ACGCACA309.682426E-750.51371433
GCAATAG150.00807734850.51371433
AGTCATC204.0076184E-450.39656431
GATTTAC150.00818181850.3498648
CGGATTT150.00818181850.3498646
ACAGACC150.00818181850.3498646
TATTCTA204.0260117E-450.34985746
TGCTATT204.0260117E-450.34985748
TCTTGAA150.0082118550.3032442
CGGCCAA150.0082118550.3032445
TTCAACC150.0082118550.3032443
CCTCGGA150.0082118550.3032443
GTTACCC150.0082118550.3032444
CTCGGAT150.0082118550.3032444
ACTATTC204.0444723E-450.30323844
ATCCGTG204.0444723E-450.30323845