FastQCFastQC Report
Fri 17 Jun 2016
SRR1526493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301912
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC22930.7594928323484988No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT13870.45940538964996425No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC12020.39812925620710665No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.32791011950502136No Hit
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC8640.28617610429529133No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8090.2679588754339013No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7860.2603407615464109No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC7680.2543787593735923No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC7330.24278597737088953No Hit
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.18846551312965365No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC5680.1881342907867193No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT5620.1861469567291131No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA4520.149712499006333No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4460.14772516494872678No Hit
GCGAATGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT4360.14441294151938314No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.11725270939876521No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC3420.11327804128355283No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC3360.11129070722594664No Hit
GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3350.11095948488301227No Hit
GCGAATGGGAGTTTAGGCTCCTGGGCAGGTTTACAGATGTATGTGACTTCGGCACTTTAAAAGC3250.10764726145366862No Hit
GCGAATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3130.10367259333845624No Hit
GCGAATGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGACCCGGT3120.10334137099552188No Hit
GCGAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3100.10267892630965315No Hit
GCGAATGGGAGGTTAACGCTGAGGAAAGCTGAGCTAAGTATTCTTCCTGTGAGTCAGTTAGGAC3100.10267892630965315No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGC3070.10168525928085007No Hit
GCGAATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA3040.10069159225204695No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3040.10069159225204695No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG3030.1003603699091126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCA150.00464564158.120250
CGACATT150.00464564158.120249
ACGACCG150.00464564158.120253
GTCTAAG150.004648758.1105444
ATTAGCG150.004651760758.10088340
AATCGTC150.004651760758.10088339
CTAGACT150.00466708658.05264337
ATAATCG150.00466708658.05264337
GCTAGAC150.00467629958.02373536
TGTCGTA150.00467629958.02373535
GATAGAC150.00467629958.02373535
ATGATTC150.0047040257.9371924
CACCTAG150.00470710857.92759320
AATGCGG150.004716379657.8988084
TACCGGA150.004716379657.89880810
ATGGGAT16400.055.4275175
ACGTACT1000.055.19583541
AATGGGG201500.055.125984
ATGGGGG88400.054.820485
GCGAATG317650.054.754611
Produced by FastQC (version 0.11.5)