FastQCFastQC Report
Fri 17 Jun 2016
SRR1526492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293131
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC18570.6335051563976515No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT15110.5154691929546859No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9760.3329569373420075No Hit
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC8200.2797384104717686No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.2749623888295677No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7030.23982451531908944No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6220.21219181867492692No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT5350.18251225561267828No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5320.18148882240363523No Hit
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.1545384145655014No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC4330.14771552650521438No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC4090.13952806083286995No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4040.13782233881779818No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA3620.12349427389119541No Hit
GCGAATGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT3550.12110626307009494No Hit
GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3180.10848392015856392No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2940.10029645448621947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAC150.004597474458.2728936
AGTATTC201.965154E-458.252837
ATTAGTA150.004610040758.2327358
CTTACCG150.004610040758.2327354
TAAGTAT201.9701823E-458.222735
ATCCGTA201.978586E-458.172650
AATTCGT201.9819554E-458.15258849
TACTAGA150.004644729758.12259341
CGTTAAA550.058.112631
ATATTAG258.624482E-658.0327927
CTTATGG150.004721089357.88373622
AACCGTA150.004743535557.81443812
GCTTATG150.004743535557.81443821
TACTATA150.004743535557.81443811
ATAGGAT150.004772512257.7255825
GGATAGC258.9001915E-657.7255828
AATGCGA150.004772512257.7255824
CGAATAG950.057.725582
GATTGTA202.0572443E-457.7157259
AATGGGG195550.055.304974
Produced by FastQC (version 0.11.5)