##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526492_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 293131 Sequences flagged as poor quality 0 Sequence length 64 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.02952604808089 39.0 38.0 40.0 35.0 40.0 2 37.4820506872354 39.0 38.0 40.0 33.0 40.0 3 37.81346565187578 39.0 38.0 40.0 35.0 40.0 4 36.410649163684496 39.0 36.0 39.0 31.0 40.0 5 37.990864152887276 39.0 38.0 40.0 35.0 40.0 6 37.81044652390911 39.0 38.0 40.0 35.0 40.0 7 38.05895657572894 39.0 38.0 40.0 35.0 40.0 8 38.01947252252406 39.0 38.0 40.0 35.0 40.0 9 38.18487638632557 39.0 38.0 40.0 36.0 40.0 10 37.4073230057551 39.0 38.0 40.0 33.0 40.0 11 37.40956773592694 39.0 38.0 39.0 33.0 40.0 12 37.156547755099254 39.0 36.0 39.0 33.0 40.0 13 37.115948159696515 39.0 36.0 39.0 32.0 40.0 14 37.080759114525584 39.0 36.0 39.0 33.0 40.0 15 36.93536336996087 39.0 36.0 39.0 33.0 40.0 16 36.60913038880228 38.0 36.0 39.0 32.0 40.0 17 36.1980309145058 38.0 36.0 39.0 30.0 40.0 18 36.48721902494107 38.0 36.0 39.0 31.0 40.0 19 36.38995193275362 38.0 35.0 39.0 31.0 40.0 20 36.212829758708565 38.0 35.0 39.0 30.0 40.0 21 36.224039763791616 38.0 35.0 39.0 31.0 40.0 22 36.16638294823816 38.0 35.0 39.0 30.0 40.0 23 35.996117776693694 38.0 35.0 39.0 30.0 40.0 24 35.80897619153211 38.0 35.0 39.0 29.0 40.0 25 35.740273120209054 38.0 35.0 39.0 29.0 40.0 26 35.938747522438774 38.0 35.0 39.0 30.0 40.0 27 35.41204785573685 38.0 35.0 39.0 28.0 40.0 28 35.28177504255776 38.0 35.0 39.0 28.0 40.0 29 35.144167624713866 38.0 35.0 39.0 27.0 40.0 30 35.033558374924525 38.0 35.0 39.0 27.0 40.0 31 34.52185200473509 38.0 34.0 39.0 26.0 40.0 32 34.70791898502717 38.0 34.0 39.0 26.0 40.0 33 34.639959608502686 38.0 34.0 39.0 26.0 40.0 34 33.85331814103592 38.0 33.0 39.0 23.0 40.0 35 33.99293489941357 38.0 33.0 39.0 23.0 40.0 36 33.083808945488535 37.0 32.0 39.0 18.0 40.0 37 32.72331142049118 37.0 32.0 39.0 17.0 40.0 38 32.67496443569598 37.0 32.0 39.0 17.0 40.0 39 32.2072315790551 36.0 31.0 39.0 11.0 40.0 40 32.229044352183834 36.0 31.0 39.0 11.0 40.0 41 31.590548253170084 36.0 30.0 39.0 4.0 40.0 42 31.2661949776721 36.0 30.0 39.0 4.0 40.0 43 31.203441464737608 36.0 30.0 39.0 4.0 40.0 44 31.040309622660175 36.0 30.0 39.0 4.0 40.0 45 30.771358198211722 36.0 29.0 39.0 4.0 40.0 46 30.270762901228462 35.0 29.0 39.0 4.0 40.0 47 30.083286994551923 35.0 28.0 39.0 4.0 40.0 48 29.84457119854263 35.0 28.0 38.0 4.0 40.0 49 29.615086770078907 35.0 27.0 38.0 4.0 40.0 50 29.239667588893703 35.0 27.0 38.0 4.0 39.0 51 28.69057179213389 35.0 25.0 38.0 4.0 39.0 52 28.53412296891151 34.0 25.0 38.0 4.0 39.0 53 28.251416602133517 34.0 24.0 38.0 4.0 39.0 54 27.970456894698955 34.0 23.0 38.0 4.0 39.0 55 27.75184132691527 34.0 22.0 38.0 4.0 39.0 56 27.120099887081203 33.0 16.0 38.0 4.0 39.0 57 26.69050356325329 33.0 14.0 37.0 4.0 39.0 58 26.38080243986477 33.0 10.0 37.0 4.0 39.0 59 26.049950363489362 33.0 4.0 37.0 4.0 39.0 60 25.85093013021482 33.0 4.0 37.0 4.0 39.0 61 25.50682118233827 33.0 4.0 37.0 4.0 39.0 62 25.10768905369954 32.0 4.0 36.0 4.0 39.0 63 24.886702532314903 32.0 4.0 36.0 4.0 39.0 64 24.783137914447806 32.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.027507194224916987 2 2 -0.25966496235702863 2 3 -0.23230074054941952 2 4 -0.25227509020571404 2 5 -0.2279596182977741 2 6 -0.1279875068649261 2 7 0.017376177648252167 2 8 0.38753878767822414 2 9 -0.14650522200906124 2 10 0.2661485353433406 2 11 -0.27956571700767796 2 12 -0.025099120045751988 2 13 0.07075984884897935 2 14 -0.0946107716281972 2 15 -0.01672123236809142 2 16 -0.29629255685209444 2 17 0.06195868066066623 2 18 -0.42785743780936514 2 19 -0.09454353096975154 2 20 -0.6083729285737576 2 21 -0.1158430503021961 2 22 -0.4535678855366001 2 23 -0.35203880411739874 2 24 -0.8859563233134153 2 25 -0.24284779191234662 2 26 -0.19133289193271708 2 27 0.09633607030686164 2 28 0.12725350318385864 2 29 0.5351808138685854 2 30 0.21069838749134107 2 31 0.34419338427063906 2 32 0.320749716685917 2 33 -0.12889274546667195 2 34 0.09339976687954987 2 35 0.021659297048387316 2 36 -0.7920454020861669 2 37 -1.2945826283030009 2 38 -1.3355091159099537 2 39 -4.760702156459853 2 40 -1.706829408631588 2 41 -1.9885603816804291 2 42 -2.270353621572621 2 43 -2.346891228962548 2 44 -1.7857291794907582 2 45 -2.3026147236257266 2 46 -0.7153326460406362 2 47 -0.4421390091705817 2 48 -0.344621165736541 2 49 -0.1420713273192682 2 50 -0.8398357813787918 2 51 -0.021721097704705983 2 52 -1.0609406076024044 2 53 -0.4657136153353427 2 54 -0.7387659236013349 2 55 -0.9627881676024508 2 56 -0.5788113554737677 2 57 -0.43857341118797777 2 58 -0.2500072532227051 2 59 -0.1279619008256141 2 60 0.06181093425495021 2 61 -0.04369698174321712 2 62 0.24970719038911682 2 63 -0.4302683572301227 2 64 0.13865327381089543 3 1 -0.19796678465279882 3 2 0.19785719907148547 3 3 0.12467144986213441 3 4 0.17388882660586802 3 5 0.009503445988038095 3 6 0.23338162406776775 3 7 0.24415085918816715 3 8 0.30563693417693116 3 9 0.1674023998257752 3 10 0.1429210961723939 3 11 -0.1402208752833971 3 12 -0.22930943801100057 3 13 -0.38491022509313666 3 14 -0.13617670452889996 3 15 0.10150132916786703 3 16 0.431492996865515 3 17 -0.0787010017982297 3 18 -0.11240876608127337 3 19 -0.33766182422165514 3 20 0.06844487408702804 3 21 -0.2016894260073272 3 22 -0.34396954817391645 3 23 -0.05994622801922844 3 24 -0.29604525178890384 3 25 -0.745965236879897 3 26 0.20631453281739454 3 27 0.7271627234009799 3 28 0.5008237906108732 3 29 0.44143125568334796 3 30 0.27195865526643814 3 31 0.5430170966456913 3 32 0.3529562456477535 3 33 0.10659098868101324 3 34 1.2774491935807148 3 35 0.4446704237113721 3 36 -3.71985640433412 3 37 -3.306840337086985 3 38 -3.690883704138596 3 39 -19.28610120355377 3 40 -4.8598599044537885 3 41 -3.6997195622377532 3 42 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4 19 -0.15584752690934067 4 20 -0.1476670203994317 4 21 -0.3986727187475765 4 22 -0.6642703643085994 4 23 -0.5072535532909157 4 24 -0.778917817984123 4 25 -0.07951221803615738 4 26 -0.003387926981204714 4 27 0.26038495986639276 4 28 0.5655448377700694 4 29 0.08278777692706285 4 30 -0.3149604006950497 4 31 -0.7755887800937558 4 32 -1.0469782356865451 4 33 -1.556059098914595 4 34 -1.006944545113754 4 35 -5.104432967591734 4 36 -0.7862154181357681 4 37 -0.9757716706766963 4 38 -0.9762174932539516 4 39 -0.7384933470111648 4 40 -0.42871859778370336 4 41 -0.47897863158783593 4 42 -0.17796552297044244 4 43 -0.07696983086380627 4 44 -0.07032841953520474 4 45 0.020270363961209625 4 46 -0.09048182504172075 4 47 0.33204170661426957 4 48 0.2983506171411392 4 49 -0.4655599897151852 4 50 -0.409061696686269 4 51 -0.2170598540662496 4 52 -0.060597667370025476 4 53 -0.18764395731534478 4 54 0.2820711249178487 4 55 0.12885918009779118 4 56 -0.26187971032088697 4 57 0.06545270714012119 4 58 0.04887201809788877 4 59 0.08024397305468511 4 60 0.12112587684675447 4 61 0.3250597913263711 4 62 0.2791314623270047 4 63 0.6147665540855343 4 64 0.06118993412173879 5 1 -0.28539147379521523 5 2 -0.09355843140323827 5 3 -0.49685855341379437 5 4 -0.10861056805863001 5 5 -0.4465634400237235 5 6 -0.07645044874341522 5 7 -0.2657552782016239 5 8 -0.1688748805476763 5 9 -0.2439551184411144 5 10 -0.26259671493443904 5 11 -1.5506668295057935 5 12 -1.693722832305177 5 13 -1.738853451744994 5 14 -1.4484016329568803 5 15 -1.3038170773042381 5 16 -18.030157218918788 5 17 -2.954927696381546 5 18 -3.356978550581836 5 19 -3.1855873038610127 5 20 -3.4004551244886514 5 21 -3.0455128674464618 5 22 -3.340850289959157 5 23 -3.4180342224358924 5 24 -3.5298471860138605 5 25 -2.8081367533521444 5 26 -0.28219833590313925 5 27 0.2306451829147207 5 28 0.37595375970315814 5 29 0.06048294421331235 5 30 0.23150800079194056 5 31 -0.35551443065138244 5 32 -0.4967923620805976 5 33 -0.5932338201041816 5 34 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0.3035803696888699 118 56 -0.00909160623166727 118 57 -0.09811606609407875 118 58 -0.21506850234820973 118 59 -0.29522071096018365 118 60 -0.046160368506402705 118 61 -0.10988444659184537 118 62 -0.18555255254288383 118 63 -0.4224081671040594 118 64 -0.36631935955855965 119 1 0.11962441974500138 119 2 0.4316153200110122 119 3 0.2275366839117794 119 4 0.3306437824612729 119 5 0.1343993313786811 119 6 0.3243361468063952 119 7 0.29420916336941616 119 8 0.5259018017431458 119 9 0.1960130944784524 119 10 0.09252962524119823 119 11 0.04947594297414071 119 12 0.18377568609361106 119 13 0.2391110975788564 119 14 0.17504268577594218 119 15 -0.1994315377320106 119 16 0.12589579546621366 119 17 0.7498430595044496 119 18 0.17661338950760097 119 19 0.2812787055134791 119 20 0.017262132124677976 119 21 0.93199373241346 119 22 0.07794449480459775 119 23 0.2308666988506758 119 24 0.1267849997519903 119 25 0.1761986811610825 119 26 0.49746062643725253 119 27 1.2563981057098275 119 28 0.7966342187301478 119 29 1.266102253517765 119 30 0.5784720652281194 119 31 0.7048944912817063 119 32 0.6750868470137377 119 33 0.4952716483511779 119 34 1.0643474111386055 119 35 0.6694746882457778 119 36 0.3875898725274496 119 37 0.06809719414739845 119 38 0.010057491930034246 119 39 0.47685065385086034 119 40 0.111904213487243 119 41 -0.324407218409668 119 42 -0.022639840086977614 119 43 0.08418797313382242 119 44 -0.03249371235347809 119 45 0.2802105611488592 119 46 0.050202944097872404 119 47 0.7131634134188616 119 48 -0.08519552800190056 119 49 0.3490378229026696 119 50 -0.021703795711108143 119 51 0.5556894590095567 119 52 0.40742668476617183 119 53 0.36942462735143167 119 54 0.7231438041915936 119 55 0.7142667551206898 119 56 0.4062012120432108 119 57 0.6575948331031611 119 58 0.4855133628530197 119 59 0.3611016854624225 119 60 0.3738062281209338 119 61 0.2280822577883086 119 62 -0.22959651569141926 119 63 -0.10702914657714757 119 64 -0.2504985524899759 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 255.0 5 39.0 6 102.0 7 132.0 8 344.0 9 317.0 10 503.0 11 601.0 12 714.0 13 1037.0 14 1306.0 15 1589.0 16 2019.0 17 2243.0 18 2316.0 19 2781.0 20 3184.0 21 4147.0 22 5674.0 23 4788.0 24 5548.0 25 5148.0 26 5228.0 27 5831.0 28 6556.0 29 7745.0 30 9048.0 31 10383.0 32 12473.0 33 15833.0 34 19593.0 35 24965.0 36 31404.0 37 38521.0 38 38756.0 39 21991.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.4637191689694 0.4124449902773514 0.055265581823764204 0.0685702589294852 8 99.49991636826635 0.21266355124235792 0.2034469928418063 0.08397308764947038 9 99.3535313562878 0.3387563921932515 0.09245013321688937 0.2152621183020561 10 64.34733958537309 24.077630820349945 4.172537193268538 7.402492401008423 11 39.51714421197349 27.324643248240548 15.859803296137223 17.298409243648745 12 39.28857746195387 15.813066512924257 23.59525263448765 21.30310339063422 13 27.91823450948552 15.221522118097369 27.951325516577914 28.9089178558392 14 24.699195922642094 18.274423380672804 32.09213628036612 24.934244416318986 15 27.71898913121421 17.154105928832163 30.62416128007867 24.502743659874955 16 32.92434096042692 18.683763366748785 24.358020731682352 24.033874941141946 17 35.063005332140065 18.28128148985664 22.031200363446317 24.624512814556983 18 33.601017974898596 19.103745424400696 22.41455185565498 24.88068474504573 19 31.253944482159852 21.189843448833457 22.132084289959096 25.424127779047595 20 29.13627940468837 24.70556918740167 21.6372093658177 24.52094204209226 21 29.418928738345656 21.524506108190536 25.965523946631368 23.09104120683244 22 29.223111851015414 18.595099119506294 27.31065632771696 24.87113270176133 23 31.205267287551596 21.015931497970183 24.372462729846823 23.40633848463139 24 30.98980277634681 24.17891709510472 20.625070363913633 24.206209764634842 25 30.833324799716 19.967299178383325 22.011612546465546 27.18776347543513 26 27.52656398217466 18.63744003057332 23.937952543113155 29.89804344413886 27 25.721620586867942 17.89044767139771 26.69585965488008 29.692072086854264 28 27.913898685037864 21.01547335903114 22.333511030919382 28.73711692501162 29 30.277720844435336 23.435130149620825 21.81697069071531 24.47017831522853 30 30.443076270925708 20.201830283430706 25.134092020114657 24.221001425528932 31 30.47671798659049 19.956304555198834 26.538999381152152 23.027978077058524 32 34.44259908552213 19.49519181480798 25.106108138032845 20.956100961637038 33 34.92708778528079 19.13018206684332 24.326181724933754 21.61654842294213 34 27.053054067752498 21.611005328694127 31.661895042533665 19.674045561019714 35 25.996833116165813 28.806673027794023 26.75709043402724 18.43940342201292 36 28.985715606034155 25.989176311114303 24.37691904825476 20.648189034596783 37 27.67707962420225 27.961256128625816 24.197800542332025 20.1638637048399 38 30.749008157243445 24.68498937127952 24.71750880592059 19.84849366555644 39 29.719056171595177 22.166437154228923 24.1784771134318 23.936029560744103 40 29.05671230906719 21.249567715448908 25.231721639565418 24.46199833591848 41 24.466375946752354 23.34072122746378 26.28674392554099 25.906158900242875 42 23.249801113762928 20.901914794392777 28.651619894110226 27.196664197734066 43 24.03148882770633 19.778577036882112 30.851782389708532 25.33815174570303 44 26.43742374182581 19.11766721960983 28.026335631931783 26.41857340663258 45 25.896453954202762 20.210369689759563 26.656198466111487 27.236977889926184 46 24.17845446907738 21.83563945613223 26.921111739793908 27.064794334996485 47 23.53616926426967 21.21427224251153 29.04960307254428 26.19995542067452 48 25.211124178390072 23.424754759937322 28.812664364844526 22.55145669682808 49 28.510185883805473 20.84127855134097 27.78551340969888 22.863022155154674 50 23.332703895078367 20.973340428819117 27.954955109608086 27.739000566494433 51 22.621604374944248 24.359299844242106 28.80285990709419 24.21623587371946 52 23.3303482894326 25.158773455754446 27.16595814762586 24.344920107187093 53 25.749370233854307 21.841044965646 28.228280789901778 24.181304010597916 54 23.96636925188744 20.88984214138641 28.803363074811255 26.340425531914896 55 26.697216215288506 20.728383619949888 26.065286788178355 26.509113376583255 56 23.78110955851807 22.42051162327608 24.599253610923235 29.199125207282616 57 25.234209523547968 25.05810181291388 24.802350849127528 24.905337814410622 58 23.84164877629884 28.2713610991842 24.12056676685273 23.766423357664234 59 27.149781354878378 26.471689493011258 24.18321533710509 22.195313815005274 60 30.016082805831047 23.701519618961218 22.812841501886638 23.469556073321098 61 28.109646608048394 22.436423438610465 23.171497924556157 26.28243202878498 62 24.80752721403798 22.35706191408262 24.670169293636896 28.165241578242505 63 23.949011339207836 22.2347373987816 24.13547983873737 29.680771423273196 64 24.490399122912535 21.58556242546303 24.372514718366254 29.55152373325818 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 4.0 6 8.0 7 14.0 8 20.0 9 161.5 10 313.5 11 324.0 12 297.0 13 277.0 14 284.0 15 444.0 16 812.5 17 1021.0 18 1116.0 19 1211.0 20 1359.5 21 1847.5 22 2187.0 23 2557.0 24 3301.5 25 3676.0 26 3851.0 27 4240.0 28 4454.0 29 4103.0 30 3942.5 31 4133.0 32 3773.0 33 3413.0 34 3259.5 35 3066.0 36 3026.0 37 3083.0 38 3252.0 39 3364.0 40 3528.5 41 3730.0 42 3767.0 43 3958.0 44 4149.0 45 4474.0 46 5009.5 47 5220.0 48 5929.0 49 6761.5 50 6885.0 51 7178.5 52 8164.5 53 8857.0 54 10569.0 55 13636.0 56 14991.0 57 15184.5 58 15378.0 59 17729.5 60 22206.0 61 24331.0 62 25382.0 63 23186.0 64 19939.0 65 18405.0 66 15020.0 67 13169.0 68 11291.5 69 9414.0 70 8157.0 71 5936.0 72 4972.0 73 4502.0 74 3231.0 75 2430.0 76 1962.0 77 1208.0 78 922.0 79 736.5 80 429.0 81 307.0 82 242.5 83 178.0 84 144.5 85 82.5 86 54.0 87 39.0 88 20.0 89 16.0 90 12.0 91 6.0 92 4.0 93 3.0 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 3.4114440301435196E-4 8 0.06140599254258335 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.09347356642593244 16 0.01808065335976065 17 0.12895258433942505 18 0.0 19 0.0 20 0.03684359552555001 21 0.0 22 0.0 23 3.4114440301435196E-4 24 0.004093732836172223 25 0.05867683731846853 26 0.02217438619593288 27 0.20298091979353944 28 0.17023105710416161 29 0.13338746157861162 30 0.207415797032726 31 0.22242615076535746 32 0.17227792352224774 33 0.22379072837741487 34 0.3844697421971746 35 0.24903541420047692 36 0.33909753659626585 37 0.3612719227921987 38 0.34046211420832323 39 0.6597732754297566 40 0.3677536664494714 41 0.41346701645339456 42 0.5141046153426284 43 0.502164561237126 44 0.4636152436965043 45 0.4960239619828677 46 0.5171749149697575 47 0.5171749149697575 48 0.5042114276552122 49 0.5291149690752599 50 0.6365754560247807 51 0.5639116981827238 52 0.5724403082580826 53 0.598026138484159 54 0.5905209616178432 55 0.6144010698288478 56 0.6352108784127233 57 0.6249765463222928 58 0.6843356724467899 59 0.689111694088991 60 0.7290255892416702 61 0.6372577448308094 62 0.6553383981905702 63 0.6580675534146849 64 0.739601065735115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 293131.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.387162970609694 #Duplication Level Percentage of deduplicated Percentage of total 1 82.86006447300255 44.23663765775413 2 6.88765175668999 7.354243736384292 3 3.090890393982408 4.950416075634925 4 1.8439392747525998 3.9377074627649957 5 1.2295267806495802 3.282047330763411 6 0.8440890438854827 2.7038111608572226 7 0.6143243843443325 2.2957925216667237 8 0.4470200880230203 1.9092107432337013 9 0.3525411942124984 1.693905677034841 >10 1.6565471069483715 15.362798899336186 >50 0.10837843969326243 3.9576359169247426 >100 0.059608141831294335 5.627866279525536 >500 0.004214717099182428 1.6052983893049193 >1k 0.0012042048854806937 1.0826281488143707 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 1857 0.6335051563976515 No Hit GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 1511 0.5154691929546859 No Hit GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 976 0.3329569373420075 No Hit GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 820 0.2797384104717686 No Hit GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 806 0.2749623888295677 No Hit GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 703 0.23982451531908944 No Hit GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 622 0.21219181867492692 No Hit GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 535 0.18251225561267828 No Hit GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 532 0.18148882240363523 No Hit GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 453 0.1545384145655014 No Hit GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC 433 0.14771552650521438 No Hit GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC 409 0.13952806083286995 No Hit GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 404 0.13782233881779818 No Hit GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA 362 0.12349427389119541 No Hit GCGAATGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCT 355 0.12110626307009494 No Hit GCGAATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 318 0.10848392015856392 No Hit GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG 294 0.10029645448621947 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0030702996271291675 0.0 0.0 0.0 0.0 9 0.0030702996271291675 0.0 0.0 0.0 0.0 10 0.007164032463301391 0.0 0.0 0.0 0.0 11 0.009552043284401854 0.0 0.0 0.0 0.0 12 0.010916620896459263 0.0 0.0 0.0 0.0 13 0.01228119850851667 0.0 0.0 0.0 0.0 14 0.016374931344688894 0.0 0.0 0.0 0.0 15 0.0177395089567463 0.0 0.0 0.0 0.0 16 0.019786375374832414 0.0 0.0 0.0 0.0 17 0.02115095298688982 0.0 0.0 0.0 0.0 18 0.023197819404975933 0.0 0.0 0.0 0.0 19 0.027632696644162506 0.0 0.0 0.0 0.0 20 0.03172642948033473 0.0 0.0 0.0 0.0 21 0.0354790179134926 0.0 0.0 0.0 0.0 22 0.04025503955569353 0.0 0.0 0.0 0.0 23 0.04366648358583705 0.0 0.0 0.0 0.0 24 0.048442505228037974 0.0 0.0 0.0 0.0 25 0.052195093661195846 0.0 0.0 0.0 0.0 26 0.05390081567626761 0.0 0.0 0.0 0.0 27 0.056971115303396776 0.0 0.0 0.0 0.0 28 0.06686430299081299 0.0 0.0 0.0 0.0 29 0.0695934582149278 0.0 0.0 0.0 0.0 30 0.07641634627521483 0.0 0.0 0.0 0.0 31 0.0788043570963153 0.0 0.0 0.0 0.0 32 0.08085122351440141 0.0 0.0 0.0 0.0 33 0.08426266754454494 0.0 0.0 0.0 0.0 34 0.08630953396263104 0.0 0.0 0.0 0.0 35 0.10097874329224818 0.0 0.0 0.0 0.0 36 0.10473133172540605 0.0 0.0 0.0 0.0 37 0.10677819814349215 0.0 0.0 0.0 0.0 38 0.11121307538267873 0.0 0.0 0.0 0.0 39 0.11462451941282226 0.0 0.0 0.0 0.0 40 0.11803596344296577 0.0 0.0 0.0 0.0 41 0.12349427389119541 0.0 0.0 0.0 0.0 42 0.13099945075751115 0.0 0.0 0.0 0.0 43 0.1340697503846403 0.0 0.0 0.0 0.0 44 0.1385046276238269 0.0 0.0 0.0 0.0 45 0.1439629380720565 0.0 0.0 0.0 0.0 46 0.14942124852028615 0.0 0.0 0.0 0.0 47 0.15283269255042967 0.0 0.0 0.0 0.0 48 0.1569264253866019 0.0 0.0 0.0 0.0 49 0.16067901381975977 0.0 0.0 0.0 0.0 50 0.16920762389511856 0.0 0.0 0.0 0.0 51 0.17193677911923338 0.0 0.0 0.0 0.0 52 0.17807737837349172 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGTAC 15 0.0045974744 58.27289 36 AGTATTC 20 1.965154E-4 58.2528 37 ATTAGTA 15 0.0046100407 58.23273 58 CTTACCG 15 0.0046100407 58.23273 54 TAAGTAT 20 1.9701823E-4 58.2227 35 ATCCGTA 20 1.978586E-4 58.1726 50 AATTCGT 20 1.9819554E-4 58.152588 49 TACTAGA 15 0.0046447297 58.122593 41 CGTTAAA 55 0.0 58.1126 31 ATATTAG 25 8.624482E-6 58.03279 27 CTTATGG 15 0.0047210893 57.883736 22 AACCGTA 15 0.0047435355 57.814438 12 GCTTATG 15 0.0047435355 57.814438 21 TACTATA 15 0.0047435355 57.814438 11 ATAGGAT 15 0.0047725122 57.725582 5 GGATAGC 25 8.9001915E-6 57.725582 8 AATGCGA 15 0.0047725122 57.725582 4 CGAATAG 95 0.0 57.72558 2 GATTGTA 20 2.0572443E-4 57.715725 9 AATGGGG 19555 0.0 55.30497 4 >>END_MODULE