FastQCFastQC Report
Fri 17 Jun 2016
SRR1526491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274826
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC21740.7910459709052273No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC17240.6273060045265004No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12600.45847190586043535No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC11740.4271793789525008No Hit
GCGAATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10190.37078005719982826No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC9700.35295059419414465No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT8250.30018993836099933No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC7560.27508314351626123No Hit
GCGAATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6550.23833261772903586No Hit
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA6540.23796875113708307No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC6150.22377795405092676No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC4870.17720303028097778No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4470.16264836660286872No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA4220.15355170180405056No Hit
GCGAATGGGAGTTTAGGCTCCTGGGCAGGTTTACAGATGTATGTGACTTCGGCACT3930.1429995706374215No Hit
GCGAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3920.14263570404546877No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.13790543835008334No Hit
GCGAATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3720.13535837220641425No Hit
GCGAATGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3520.12808104036735973No Hit
GCGAATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3440.12517010763173791No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG3140.11425410987315611No Hit
GCGAATGGGAGGTTAACGCTGAGGAAAGCTGAGCTAAGTATTCTTCCTGTGAGTCA3080.11207091032143975No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2890.10515744507433794No Hit
GCGAATGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGA2880.10479357848238523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTA309.663927E-750.56939334
TACGACG203.95114E-450.56939334
CTACGAC204.0055707E-450.42941733
AATCCGA150.00816791150.3922232
TATGCGT150.00816791150.3922232
CGTCATT150.00817386850.38292731
CAACCTA150.00823360950.2902150
AATCTGG150.00823360950.2902150
CCAATTA150.00825159450.26246349
TAGCTTC150.00825159450.26246347
TAATTCA150.00825759650.2532238
CGTAAAT150.00825759650.2532238
CTTATGC150.00826360150.2439844
AATGCAA252.0241112E-550.24397743
CGATCCA204.07907E-450.24397745
CCGACTA150.00848807649.90449529
ATATTGG204.2407232E-449.84987324
ACGTGTA204.2636783E-449.79537223
ATAGGGT252.1482756E-549.740995
TCCGATC150.00861128649.7228913
Produced by FastQC (version 0.11.5)