FastQCFastQC Report
Fri 17 Jun 2016
SRR1526490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68278
Sequences flagged as poor quality0
Sequence length64
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.6868976830018454No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4660.682503881191599No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3170.4642783912826972No Hit
GTCCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2340.34271654119921496No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2250.32953513576847593No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2240.3280705351650605No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2130.31195992852749055No Hit
GTCCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTTCCTCATGTTGGGATTACAG1940.284132517062597No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1680.24605290137379537No Hit
GTCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1600.23433609654647178No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1560.22847769413280997No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.2196900905123173No Hit
GTCCCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1480.21676088930548643No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1400.2050440844781628No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.199185682064501No Hit
GTCCCGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1150.1684290693927766No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1130.1654998681859457No Hit
GTCCCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1100.16110606637569935No Hit
GTCCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1090.1596414657722839No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC1080.15817686516886845No Hit
GTCCCGGGGGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACCCTAGCTGCAGTGGTGCCT1070.156712264565453No Hit
GTCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1060.15524766396203754No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC1030.1508538621517912No Hit
GTCCCGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1020.14938926154837576No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1000.14646006034154485No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT1000.14646006034154485No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC950.13913705732446763No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG930.13620785611763672No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA880.12888485310055947No Hit
GTCCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG870.12742025249714403No Hit
GTCCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTTC850.12449105129031313No Hit
GTCCCGGGGAACTACAAGCCTCCAGCCCAGAAGAGCATCCAGGAGATCCAGGAACTGGACAAGG850.12449105129031313No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC810.11863264887665133No Hit
GTCCCGGGGCAAGTGGAGTATATTGAATATTGAAGTAGACTTCAGGTTGTTTTTTGGTTTTGTT810.11863264887665133No Hit
GTCCCGGGGATGTTTTCTAGGTCTGAAGTGTTCCCAAATGTAGGAAAGGGACCTGGGGCAGAAA790.11570344766982045No Hit
GTCCCGGGGCAAGCAGAAAATGAGTCCACTCGACAGTATGAAGGTCCACTGCATCATTATCGGT790.11570344766982045No Hit
GTCCCGGGGACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCA770.11277424646298954No Hit
GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC770.11277424646298954No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCTGAAGGTCT730.10691584404932775No Hit
GTCCCGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCCGGAGCTT730.10691584404932775No Hit
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA730.10691584404932775No Hit
GTCCCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC690.10105744163566596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAG303.5678022E-758.3259458
CTAAAGA150.004565796358.3259458
TCGACGC303.5678022E-758.3259458
TATTTCC150.00457918658.28286752
AACGTCA150.00457918658.28286749
CAACCAA452.910383E-1158.28286749
TGTCTGA150.00457918658.28286753
ACGTCAT150.00457918658.28286750
GCGTGAG150.00457918658.28286754
TGGGATT303.5861194E-758.28286754
CGCAAGC351.5499609E-858.28286748
GATTACA303.5861194E-758.28286757
ATTTCCT150.00457918658.28286753
ATCCTCG201.9493788E-458.28286448
TTGTAGT201.9493788E-458.28286456
CTCATGT351.558874E-858.23985347
CAGGTTG150.004633035558.11119543
AGGTTGT150.004633035558.11119544
CACCCTA201.9780532E-458.1111943
TACGGCA150.004646571458.0684341
Produced by FastQC (version 0.11.5)