##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526489_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66644 Sequences flagged as poor quality 0 Sequence length 64 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.04786627453334 39.0 38.0 40.0 35.0 40.0 2 37.86603445171358 39.0 38.0 40.0 35.0 40.0 3 38.099888962247164 39.0 38.0 40.0 35.0 40.0 4 37.96857931696777 39.0 38.0 40.0 35.0 40.0 5 38.09355680931517 39.0 38.0 40.0 35.0 40.0 6 37.51646059660285 39.0 38.0 39.0 33.0 40.0 7 38.06675769761719 39.0 38.0 40.0 35.0 40.0 8 37.940129644078986 39.0 38.0 40.0 35.0 40.0 9 38.049051677570375 39.0 38.0 40.0 35.0 40.0 10 37.36603445171358 39.0 38.0 39.0 33.0 40.0 11 37.06356161094772 39.0 36.0 39.0 32.0 40.0 12 36.92913090450753 39.0 35.0 39.0 32.0 40.0 13 36.98631534721805 39.0 36.0 39.0 32.0 40.0 14 36.804228437668804 39.0 36.0 39.0 32.0 40.0 15 36.81035051917652 39.0 36.0 39.0 32.0 40.0 16 36.426715083128265 38.0 36.0 39.0 31.0 40.0 17 36.009123101854634 38.0 35.0 39.0 30.0 40.0 18 36.32116919752716 38.0 35.0 39.0 31.0 40.0 19 36.06774803433167 38.0 35.0 39.0 30.0 40.0 20 35.90584298661545 38.0 35.0 39.0 29.0 40.0 21 35.936378368645336 38.0 35.0 39.0 30.0 40.0 22 35.85569593661845 38.0 35.0 39.0 30.0 40.0 23 35.71940459756317 38.0 35.0 39.0 29.0 40.0 24 35.54573555008703 38.0 35.0 39.0 29.0 40.0 25 35.5135346017646 38.0 35.0 39.0 29.0 40.0 26 35.71740891903247 38.0 35.0 39.0 29.0 40.0 27 35.1969869755717 38.0 35.0 39.0 27.0 40.0 28 35.03317627993518 38.0 35.0 39.0 27.0 40.0 29 34.91309045075326 38.0 35.0 39.0 27.0 40.0 30 34.70608006722286 38.0 34.0 39.0 26.0 40.0 31 34.12898385451054 38.0 33.0 39.0 24.0 40.0 32 34.29328971850429 38.0 33.0 39.0 25.0 40.0 33 34.1768201188404 38.0 33.0 39.0 25.0 40.0 34 33.354960686633454 37.0 33.0 39.0 21.0 40.0 35 33.44191525118541 38.0 33.0 39.0 22.0 40.0 36 32.232624092191344 37.0 31.0 39.0 8.0 40.0 37 31.88747674209231 36.0 30.0 39.0 4.0 40.0 38 31.843556809315167 36.0 31.0 39.0 4.0 40.0 39 31.348388452073706 36.0 30.0 39.0 4.0 40.0 40 31.335544084988896 36.0 30.0 39.0 4.0 40.0 41 30.819083488386052 36.0 29.0 39.0 4.0 40.0 42 30.46419782726127 36.0 29.0 39.0 4.0 40.0 43 30.421298241402077 36.0 29.0 39.0 4.0 40.0 44 30.254786627453335 35.0 28.0 39.0 4.0 40.0 45 30.01569533641438 35.0 28.0 39.0 4.0 40.0 46 29.552157733629436 35.0 27.0 38.0 4.0 40.0 47 29.34399195726547 35.0 27.0 38.0 4.0 40.0 48 29.049891963267513 35.0 26.0 38.0 4.0 40.0 49 28.92173338935238 35.0 26.0 38.0 4.0 40.0 50 28.405167757037393 34.0 23.0 38.0 4.0 39.0 51 27.988761178800793 34.0 23.0 38.0 4.0 39.0 52 27.830472360602606 34.0 22.0 38.0 4.0 39.0 53 27.529109897365103 33.0 21.0 38.0 4.0 39.0 54 27.33350339115299 33.0 19.0 38.0 4.0 39.0 55 27.126823119860752 33.0 18.0 38.0 4.0 39.0 56 26.58261809015065 33.0 4.0 38.0 4.0 39.0 57 26.188839205329813 33.0 4.0 37.0 4.0 39.0 58 25.84115599303763 33.0 4.0 37.0 4.0 39.0 59 25.48263909729308 32.0 4.0 36.0 4.0 39.0 60 25.31842326390973 32.0 4.0 36.0 4.0 39.0 61 24.93477282275974 32.0 4.0 36.0 4.0 39.0 62 24.50904807634596 32.0 4.0 36.0 4.0 39.0 63 24.42294880259288 32.0 4.0 36.0 4.0 39.0 64 24.225721745393432 32.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.1724583462110445 2 2 0.406505777787757 2 3 -0.36790219511959776 2 4 0.3497494000844128 2 5 -0.16980252762846249 2 6 0.11162515560150155 2 7 -0.16113282211304636 2 8 -0.6507387214237923 2 9 -0.6293450369285836 2 10 0.09989401996700309 2 11 -1.302245762247594 2 12 -0.6969110270591088 2 13 -0.8319645189004561 2 14 -1.854867723900945 2 15 -1.7941520611820891 2 16 -1.8649836950910768 2 17 -0.6780810333647622 2 18 -2.06070107775426 2 19 -2.682873466315179 2 20 -0.7876076018678617 2 21 -1.372535137236504 2 22 -1.7146683324870509 2 23 -1.4899077567424754 2 24 -0.8432892580109339 2 25 -1.7414942453674769 2 26 -2.7174182677985925 2 27 -1.816572193469618 2 28 -3.2537515965133075 2 29 -2.542082954914946 2 30 -2.822613432280903 2 31 -2.2083752240478027 2 32 -2.3064924936549964 2 33 -2.181086288476198 2 34 -1.9890888294413323 2 35 -2.642461784364844 2 36 -2.00025795642604 2 37 -2.215929797096834 2 38 -3.2565385446696915 2 39 -5.440357585326424 2 40 -3.7496951565155356 2 41 -4.536189698122545 2 42 -3.9062437594281647 2 43 -3.9468140750833953 2 44 -3.930552718060426 2 45 -4.583471769602642 2 46 -2.8141232947435277 2 47 -3.761823963304032 2 48 -2.7474619151485875 2 49 -2.911988677465022 2 50 -3.4042213877046805 2 51 -1.7725357270267175 2 52 -2.4242396079737425 2 53 -2.1079114021491634 2 54 -1.5311023121798186 2 55 -2.4003492237294672 2 56 -3.609950280255344 2 57 -3.0753673634461407 2 58 -2.8518686627806176 2 59 -3.2275327365612867 2 60 -4.185990745877575 2 61 -2.5795460527449023 2 62 -2.8650389391473716 2 63 -3.0286423028876364 2 64 -3.317597839686691 3 1 0.40824340817491844 3 2 0.5424706900684626 3 3 0.3557820154067173 3 4 0.784837119382658 3 5 0.3442325600908376 3 6 -0.659427475977445 3 7 -0.01815036597269426 3 8 -0.240212405634324 3 9 -0.09601170359525213 3 10 -0.29747440108562984 3 11 -0.32417558680899816 3 12 -0.6574373428485814 3 13 -1.1574031153916806 3 14 -0.7855694782869094 3 15 0.2391812721512423 3 16 -0.059720537196341184 3 17 0.3341996683896227 3 18 -0.15806949880689558 3 19 -0.0872594312274586 3 20 0.22467309988652318 3 21 0.20378065223717812 3 22 0.8800685096182121 3 23 0.232899260801382 3 24 0.39092126830485796 3 25 0.5225408423518232 3 26 0.8527571707979007 3 27 2.2851821924952915 3 28 1.0488799824340589 3 29 1.1079170450850526 3 30 0.48879007649102846 3 31 1.381975653145183 3 32 0.9338583835379879 3 33 1.3768084483659067 3 34 0.2424901179270904 3 35 0.22332768931936187 3 36 -3.7520123423909517 3 37 -3.7404912006056072 3 38 -3.4074157376521477 3 39 -19.29298916427379 3 40 -7.618993402129572 3 41 -5.123908996368158 3 42 -6.138699899779041 3 43 -6.196814075083395 3 44 -6.0331842970077965 3 45 -5.243998085392114 3 46 -0.7614917157961578 3 47 -0.23550817383034683 3 48 -0.5886899853240273 3 49 -0.42339218623695274 3 50 -0.7252740192836278 3 51 -1.9085006393074195 3 52 -1.35143259042988 3 53 -2.587735963552671 3 54 -1.3626812595482392 3 55 -1.3836825570628015 3 56 -1.0678450170974472 3 57 -0.3437884160777216 3 58 -1.0781844522543018 3 59 -0.7678836137542682 3 60 -0.7166925002635374 3 61 0.7037872805884327 3 62 0.9384698327824523 3 63 0.902059451498328 3 64 -0.2790013484586211 4 1 0.374910074841587 4 2 0.3591373567351255 4 3 -0.010884651259949862 4 4 0.6348371193826594 4 5 0.444232560090839 4 6 -0.14276080931077928 4 7 0.13184963402730432 4 8 0.05978759436568026 4 9 -0.6126783702619178 4 10 0.28585893224770587 4 11 0.5258244131910033 4 12 0.025895990484748665 4 13 -0.6074031153916835 4 14 -0.018902811620243654 4 15 0.322514605484578 4 16 0.15694612947032738 4 17 0.4341996683896241 4 18 -0.0914028321402256 4 19 0.62940723543921 4 20 -0.14199356678014396 4 21 -0.7795526810961491 4 22 0.13006850961821215 4 23 0.616232594134722 4 24 -0.5090787316951406 4 25 -0.32745915764817823 4 26 0.5027571707978993 4 27 0.9851821924952944 4 28 0.5488799824340589 4 29 0.6245837117517183 4 30 0.1054567431576956 4 31 -0.4680243468548184 4 32 -0.8828082831286821 4 33 -0.5731915516340891 4 34 -0.37417654873957673 4 35 -3.493338977347303 4 36 -0.5020123423909517 4 37 -1.1738245339389373 4 38 -0.7574157376521455 4 39 -0.42632249760712426 4 40 -0.9189934021295727 4 41 0.8427576702985071 4 42 0.677966766887625 4 43 0.8698525915832711 4 44 0.9334823696588721 4 45 0.7226685812745508 4 46 0.33850828420384005 4 47 0.681158492836321 4 48 0.6613100146759727 4 49 0.3932744804297137 4 50 -0.25860735261696277 4 51 1.5414993606925798 4 52 1.2152340762367864 4 53 0.7789307031139927 4 54 1.503985407118428 4 55 0.6663174429371992 4 56 0.8988216495692178 4 57 0.08954491725561198 4 58 1.188482214412364 4 59 0.8321163862457297 4 60 0.9166408330697955 4 61 1.5871206139217655 4 62 2.35513649944912 4 63 2.768726118164995 4 64 1.7709986515413796 5 1 0.04475134468285802 5 2 0.4115183091160759 5 3 0.058162967787666275 5 4 0.41658315112869104 5 5 0.17439129024956657 5 6 -0.00784017439013951 5 7 0.15883376101142943 5 8 0.3534383880164711 5 9 0.11668670910316337 5 10 0.26363671002548017 5 11 -2.1852866979201124 5 12 -2.2566436920549293 5 13 -2.2296253376139035 5 14 -2.1831885259059547 5 15 -1.5719298389598677 5 16 -20.53749831497412 5 17 -4.083260649070688 5 18 -3.6906091813465736 5 19 -4.199164193132219 5 20 -3.7800888048753833 5 21 -3.862092363635835 5 22 -3.7088203792706764 5 23 -3.0813864534843276 5 24 -3.730507303123712 5 25 -3.1274591576481754 5 26 -0.3615285434878146 5 27 0.26613457344767255 5 28 0.5766577602118375 5 29 -0.17700358983558573 5 30 -0.19771786001690828 5 31 -1.2108814897119657 5 32 -1.7216971720175707 5 33 -1.2454137738563134 5 34 -0.9130654376284681 5 35 -4.263180247188572 5 36 -0.6702663106449229 5 37 -1.4849356450500508 5 38 -0.8137649440013526 5 39 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1.9726927407281565 6 59 1.70229182484222 6 60 2.112254868157514 6 61 2.437997806904221 6 62 1.6086452713789434 6 63 0.8660945392176274 6 64 1.9455600550501515 7 1 -1.0663597664282563 7 2 -0.6678467702489996 7 3 -0.7989798893551878 7 4 -0.39294065839511916 7 5 -0.7144975986393192 7 6 -0.4522846188345895 7 7 -0.111007508829843 7 8 0.03597807055615476 7 9 -0.10553551311906517 7 10 -0.0855696391808678 7 11 -0.02655653918994716 7 12 0.3147848793736401 7 13 -0.5312126392012075 7 14 -0.6593790020964292 7 15 0.6661653991353731 7 16 -0.12479990227570426 7 17 1.123088557278514 7 18 -0.03981553055292153 7 19 0.2770262830582553 7 20 0.6484826236960473 7 21 0.20139969985623196 7 22 0.16419549374518994 7 23 -0.35122772332560004 7 24 -1.000348572964981 7 25 -0.6671416973307203 7 26 0.1146619327026599 7 27 0.07565838297148275 7 28 0.5607847443388252 7 29 -0.22462263745462963 7 30 0.9451392828402305 7 31 -3.718817997648472 7 32 -2.689951140271539 7 33 -3.5470010754436174 7 34 -21.563859088422117 7 35 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0.4635303990868991 119 13 0.5732420458986383 119 14 0.471957403433521 119 15 0.996170519463071 119 16 -0.10380655870172006 119 17 0.9169953673143567 119 18 0.7069842646339666 119 19 0.3960739021058757 119 20 0.01176987408007335 119 21 0.7989419425597646 119 22 0.018778187037561622 119 23 0.5253723790809559 119 24 0.055975031745717274 119 25 -0.12208281356215878 119 26 0.2721120095075804 119 27 0.6916338053985172 119 28 0.8902778318964266 119 29 0.769745002074302 119 30 0.3409406141254365 119 31 1.4319756531451802 119 32 0.9306325770863708 119 33 0.460679416107844 119 34 0.8360385050238648 119 35 0.7055857538354928 119 36 0.4323962597595852 119 37 -0.25554496404646443 119 38 -1.091286705394083 119 39 0.7919570722853493 119 40 0.21917864088118222 119 41 0.5373813262124862 119 42 0.21345063785536667 119 43 1.2198525915832725 119 44 -0.03157139378198792 119 45 0.9156793339627249 119 46 1.8137771014081423 119 47 1.861266019718041 119 48 1.1430304447834985 119 49 1.0642422223651984 119 50 1.5510700667378785 119 51 0.3683810811226884 119 52 -0.19928205279547484 119 53 0.01602747730754217 119 54 -0.6465522272901758 119 55 -2.050886858138071 119 56 -0.5958020063447584 119 57 -0.23948734080890333 119 58 -0.09861455978118272 119 59 -0.14207716214136568 119 60 -0.6011011024140771 119 61 -0.18707293446533058 119 62 -0.21959468334657828 119 63 0.1660379461219854 119 64 0.4812137053048211 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 80.0 5 15.0 6 39.0 7 31.0 8 93.0 9 61.0 10 137.0 11 186.0 12 182.0 13 276.0 14 341.0 15 417.0 16 539.0 17 629.0 18 661.0 19 819.0 20 886.0 21 1284.0 22 1692.0 23 1092.0 24 1359.0 25 1034.0 26 1157.0 27 1286.0 28 1419.0 29 1708.0 30 2028.0 31 2245.0 32 2675.0 33 3466.0 34 4359.0 35 5463.0 36 7125.0 37 8471.0 38 8375.0 39 5003.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.50249084688795 1.0248484484724807 0.3601224416301542 0.11253826300942321 8 98.52579114874197 0.8316819792229628 0.35429051822494445 0.2882363538101243 9 94.73020827081207 3.4151611547926293 1.0533581417682012 0.8012724326270932 10 58.77348298421463 26.658063741672166 4.432507052397815 10.135946221715383 11 42.3518996458796 24.74791429085889 14.158813996758898 18.74137206650261 12 43.20268891423084 14.834043574815436 21.227717423924133 20.73555008702959 13 27.962007082408018 14.209831342656503 24.72390612808355 33.10425544685193 14 21.93745873597023 21.094172018486283 32.54756617249865 24.420803073044837 15 31.329028382640033 17.580717825499324 27.88857185763628 23.201681934224357 16 36.07425861447953 17.512606555408812 25.297154520350585 21.115980309761078 17 37.42733103997356 19.097477804984305 20.715347984797727 22.75984317024441 18 38.926234919872755 19.536642458435868 19.436108276814117 22.10101434487726 19 32.72612688314027 23.586519416601647 21.065662325190566 22.621691375067524 20 31.007728671118784 26.85525624671719 20.19809409469498 21.93892098746905 21 30.36432387011584 21.446791909249143 27.501350459156114 20.687533761478903 22 27.427825460656624 18.60932717123822 27.616889742512452 26.345957625592703 23 30.296800912310186 21.533821499309767 26.21391273032831 21.955464858051737 24 32.6905762304922 25.28061224489796 19.569327731092436 22.459483793517407 25 31.144169568872343 19.061486729914733 18.536087426444098 31.258256274768826 26 28.26439334814192 18.661823857837547 22.92129435072342 30.15248844329711 27 24.037464106920037 18.305094938136115 23.92170422598734 33.7357367289565 28 24.716365876748764 25.62249237381099 19.64175695373195 30.019384795708298 29 31.18098643390472 25.12056247464808 19.096194582575908 24.602256508871296 30 30.648167633707125 22.304061569912513 23.79370471695277 23.254066079427595 31 32.5087595302185 19.574730447074394 24.944736011067835 22.971774011639273 32 37.06286349769315 19.39255496611112 23.541876437083904 20.002705099111825 33 35.992422647863606 19.880025257840455 23.42664702167965 20.70090507261629 34 27.592851873598 21.64461105340018 31.34305888020716 19.419478192794664 35 25.451154956689127 30.973291626564002 24.724795476419633 18.850757940327238 36 28.93631752434564 28.710546516353347 20.748355634491787 21.604780324809223 37 29.317299298636406 29.658950663736793 20.769994882754883 20.253755154871918 38 32.76497765203389 26.576773164381706 21.107917350148234 19.550331833436168 39 30.3110333066719 22.48162624692513 21.645563889350015 25.561776557052955 40 30.21600060209227 21.548882366222625 22.081734025739443 26.153383005945663 41 24.387452374139723 24.816649850157372 22.14658976251073 28.649308013192176 42 22.609141341051618 22.143330921369994 25.85624698504583 29.391280752532563 43 22.694661320953788 19.87308956078739 28.548819823350314 28.88342929490851 44 27.08207785946728 19.70591780161504 25.7532843196336 27.458720019284076 45 27.159693754521342 20.573607427055705 24.076139377863516 28.190559440559444 46 24.74787825798574 23.149976634457392 24.05444925154891 28.04769585600796 47 24.14905484036299 19.99155838282734 27.999035243751695 27.860351533057976 48 25.785300856143735 23.27716146147353 27.52622693838177 23.411310744000964 49 30.507401489342456 19.47752419427779 24.649522144170763 25.36555217220899 50 22.297001614631277 21.0037875929922 26.91756326487498 29.781647527501548 51 22.67764507178188 24.711967667993726 27.105199662202917 25.50518759802147 52 23.08852360126678 23.890815864877094 26.593274015985525 26.42738651787061 53 25.364690974369804 21.15735642414277 28.416479355549185 25.06147324593824 54 23.562776244135705 20.67701497940897 26.66877856722632 29.091430209229006 55 27.233085883275997 20.74506608727141 24.328565393204176 27.69328263624842 56 24.159422914402995 23.15887963298322 23.003440782325242 29.678256670288544 57 26.93317638536762 25.140347700108656 22.801219364964386 25.12525654955934 58 25.340138622532958 30.300650831282184 21.201093275750114 23.158117270434744 59 28.368216000724832 27.23566186463713 22.229772583129474 22.166349551508564 60 32.023261082999774 24.01480250736349 21.08451023336606 22.877426176270674 61 29.294316501162054 22.820017506263017 21.50402945881501 26.381636533759924 62 24.678853382039943 21.883255090796563 23.14821803251468 30.289673494648817 63 25.098116169544742 21.402910276536648 21.813488709093104 31.685484844825506 64 25.603492552645097 21.269524759056164 23.032417897821684 30.09456479047705 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.5 8 5.0 9 13.0 10 28.0 11 35.0 12 75.0 13 92.0 14 69.0 15 58.5 16 57.5 17 67.0 18 97.5 19 128.0 20 134.5 21 129.5 22 118.0 23 159.5 24 217.0 25 233.0 26 308.0 27 461.5 28 540.0 29 583.5 30 677.0 31 727.0 32 693.0 33 659.0 34 800.5 35 802.5 36 663.0 37 657.5 38 658.0 39 664.0 40 629.0 41 623.5 42 653.0 43 706.5 44 760.0 45 772.0 46 871.5 47 959.0 48 1051.5 49 1199.0 50 1254.0 51 1384.0 52 1577.5 53 1641.0 54 1741.5 55 2028.0 56 2214.0 57 2493.5 58 2773.0 59 3263.5 60 3899.5 61 4045.0 62 4886.0 63 5958.5 64 6190.0 65 6097.5 66 5275.5 67 4546.0 68 4103.5 69 3661.0 70 3296.5 71 2451.5 72 1971.0 73 1719.0 74 1230.0 75 993.0 76 903.0 77 666.0 78 519.0 79 414.5 80 263.5 81 217.0 82 170.5 83 124.0 84 106.5 85 72.0 86 55.0 87 41.0 88 20.0 89 13.0 90 10.5 91 6.0 92 4.0 93 3.5 94 3.0 95 1.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.04801632555068723 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08102754936678472 16 0.018006122081507713 17 0.11253826300942321 18 0.0 19 0.0 20 0.013504591561130784 21 0.0 22 0.0 23 0.0 24 0.006002040693835904 25 0.042014284856851325 26 0.024008162775343615 27 0.19056479202928994 28 0.14554948682552069 29 0.12154132405017706 30 0.17706020046815918 31 0.2175739751515515 32 0.15455254786627454 33 0.19506632254966688 34 0.3316127483344337 35 0.22207550567192846 36 0.3076045855590901 37 0.30310305503871315 38 0.29259948382450035 39 0.5716943760878699 40 0.313606626252926 41 0.3616229518036132 42 0.46215713342536463 43 0.4471520316907749 44 0.4021367264870056 45 0.43814897065002106 46 0.46065662325190565 47 0.45915611307844667 48 0.45015305203769285 49 0.45915611307844667 50 0.562691315047116 51 0.49816937758838004 52 0.5011703979352979 53 0.5326811115779365 54 0.5296800912310186 55 0.5521877438329031 56 0.5701938659144109 57 0.5701938659144109 58 0.6317147830262289 59 0.6332152931996878 60 0.6587239661484905 61 0.5731948862613289 62 0.5957025388632134 63 0.5942020286897545 64 0.6692275373627034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 66644.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.59540243682852 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47556071361097 44.740711842026286 2 8.866157458638094 9.858351839625474 3 3.4843863863323525 5.811475901806614 4 2.0809154948584383 4.627573374947482 5 1.330598364416615 3.698757577576376 6 0.8636743947531781 2.8809795330412338 7 0.6045720763272247 2.352799951983674 8 0.41024533750775954 1.8246203709261148 9 0.3022860381636123 1.512514254846648 >10 1.449353593695177 14.604465518276214 >50 0.08366845699171413 3.2666106476201913 >100 0.04858168470486626 4.8211391873236895 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 426 0.6392173338935238 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.5942020286897545 No Hit GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 249 0.37362703319128504 No Hit GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 222 0.3331132585078927 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 185 0.27759438208991055 No Hit GTCCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 179 0.26859132104915673 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC 157 0.23558009723305923 No Hit GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 155 0.23257907688614127 No Hit GTCCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTTCCTCATGTTGGGATTACAG 150 0.22507652601884642 No Hit GTCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC 139 0.20857091411079767 No Hit GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.20857091411079767 No Hit GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC 129 0.19356581237620793 No Hit GTCCCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG 128 0.19206530220274892 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 121 0.1815617309885361 No Hit GTCCCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 116 0.17405918012124122 No Hit GTCCCGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT 111 0.16655662925394635 No Hit GTCCCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 110 0.16505611908048737 No Hit GTCCCGGGGGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACCCTAGCTGCAGTGGTGCCT 101 0.15155152751935658 No Hit GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 96 0.1440489766520617 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT 94 0.14104795630514375 No Hit GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.13954744613168477 No Hit GTCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC 90 0.13504591561130785 No Hit GTCCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 88 0.1320448952643899 No Hit GTCCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG 86 0.12904387491747193 No Hit GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG 84 0.12604285457055397 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 82 0.12304183422363604 No Hit GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC 82 0.12304183422363604 No Hit GTCCCGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC 80 0.1200408138767181 No Hit GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC 78 0.11703979352980012 No Hit GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC 74 0.11103775283596423 No Hit GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC 73 0.10953724266250527 No Hit GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA 70 0.10503571214212833 No Hit GTCCCGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG 67 0.1005341816217514 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGAAGGTCA 67 0.1005341816217514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.001500510173458976 0.0 0.0 0.0 0.0 10 0.010503571214212833 0.0 0.0 0.0 0.0 11 0.03001020346917952 0.0 0.0 0.0 0.0 12 0.04201428485685133 0.0 0.0 0.0 0.0 13 0.045015305203769285 0.0 0.0 0.0 0.0 14 0.07952703919332572 0.0 0.0 0.0 0.0 15 0.0810275493667847 0.0 0.0 0.0 0.0 16 0.08702959006062061 0.0 0.0 0.0 0.0 17 0.08702959006062061 0.0 0.0 0.0 0.0 18 0.09303163075445652 0.0 0.0 0.0 0.0 19 0.11103775283596423 0.0 0.0 0.0 0.0 20 0.12754336474401295 0.0 0.0 0.0 0.0 21 0.12904387491747193 0.0 0.0 0.0 0.0 22 0.1440489766520617 0.0 0.0 0.0 0.0 23 0.15605305803973352 0.0 0.0 0.0 0.0 24 0.15905407838665145 0.0 0.0 0.0 0.0 25 0.1635556089070284 0.0 0.0 0.0 0.0 26 0.1695576496008643 0.0 0.0 0.0 0.0 27 0.17706020046815918 0.0 0.0 0.0 0.0 28 0.21607346497809254 0.0 0.0 0.0 0.0 29 0.2190744853250105 0.0 0.0 0.0 0.0 30 0.2325790768861413 0.0 0.0 0.0 0.0 31 0.23558009723305923 0.0 0.0 0.0 0.0 32 0.24608366844727209 0.0 0.0 0.0 0.0 33 0.24758417862073104 0.0 0.0 0.0 0.0 34 0.2565872396614849 0.0 0.0 0.0 0.0 35 0.3421163195486465 0.0 0.0 0.0 0.0 36 0.35412040093631836 0.0 0.0 0.0 0.0 37 0.35562091110977734 0.0 0.0 0.0 0.0 38 0.3616229518036132 0.0 0.0 0.0 0.0 39 0.3631234619770722 0.0 0.0 0.0 0.0 40 0.3691255026709081 0.0 0.0 0.0 0.0 41 0.37362703319128504 0.0 0.0 0.0 0.0 42 0.378128563711662 0.0 0.0 0.0 0.0 43 0.3826300942320389 0.0 0.0 0.0 0.0 44 0.3886321349258748 0.0 0.0 0.0 0.0 45 0.3961346857931697 0.0 0.0 0.0 0.0 46 0.4006362163135466 0.0 0.0 0.0 0.0 47 0.4021367264870056 0.0 0.0 0.0 0.0 48 0.4066382570073825 0.0 0.0 0.0 0.0 49 0.40963927735430045 0.0 0.0 0.0 0.0 50 0.4126402977012184 0.0 0.0 0.0 0.0 51 0.4201428485685133 0.0 0.0 0.0 0.0 52 0.4261448892623492 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGATC 25 8.331419E-6 58.288097 33 AGGATCT 25 8.331419E-6 58.288097 34 TGCGATC 15 0.004590803 58.24394 32 AGTTGCA 15 0.004590803 58.24394 35 TAGGGGC 15 0.004590803 58.24394 35 AGTTAAG 25 8.368997E-6 58.24394 31 GTGCGAT 15 0.004590803 58.24394 31 AGGTTAA 20 1.970176E-4 58.155827 30 CGTCCGT 20 1.970176E-4 58.155827 50 AGTGCGA 15 0.0046184333 58.155823 30 CGTCAGA 15 0.0046184333 58.155823 30 AGGTCAT 15 0.0046322946 58.11187 48 AGCACCA 15 0.0046322946 58.11187 38 AAGGTTT 15 0.0046322946 58.11187 51 CCGCTAA 15 0.0046322946 58.11187 58 GCGATCT 15 0.0046322946 58.11187 39 ACGTCCG 15 0.0046322946 58.11187 49 GCAATTT 15 0.0046322946 58.11187 39 GTGAAAA 15 0.0046322946 58.11187 48 TTCGACT 15 0.0046322946 58.11187 58 >>END_MODULE