FastQCFastQC Report
Fri 17 Jun 2016
SRR1526488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64463
Sequences flagged as poor quality0
Sequence length56
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5530.8578564447822783No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4320.6701518700649985No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4140.6422288754789569No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3120.48399857282472114No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2770.42970386112964026No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2330.36144765214153857No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2060.3195631602624761No Hit
GTCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1920.2978452755844438No Hit
GTCCCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1880.2916401656764345No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1790.27767866838341376No Hit
GTCCCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.259063338659386No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1380.214076291826319No Hit
GTCCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTTCCTCATGTTGG1290.20011479453329817No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT1190.18460201976327506No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1190.18460201976327506No Hit
GTCCCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1100.17064052247025427No Hit
GTCCCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1090.16908924499325195No Hit
GTCCCGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG1050.1628841350852427No Hit
GTCCCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1050.1628841350852427No Hit
GTCCCGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAA1030.15978158013123805No Hit
GTCCCGGGGGGTGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAG1010.15667902517723345No Hit
GTCCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1010.15667902517723345No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC960.1489226377922219No Hit
GTCCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC920.14271752788421263No Hit
GTCCCGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC910.14116625040721034No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA900.13961497293020803No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG880.1365124179762034No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.12875603059119184No Hit
GTCCCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG800.12410219816018492No Hit
GTCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCG750.11634581077517335No Hit
GTCCCGGGGGCTGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCG750.11634581077517335No Hit
GTCCCGGGGGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACCCTAGCTGCAG750.11634581077517335No Hit
GTCCCGGGGATCTTCCTGACCTATTGTAGAACACAGGGGCACTGCATTCTGGGAAT740.11479453329817103No Hit
GTCCCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG730.11324325582116872No Hit
GTCCCGGGGCAAGCAGAAAATGAGTCCACTCGACAGTATGAAGGTCCACTGCATCA700.1085894233901618No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG700.1085894233901618No Hit
GTCCCGGGGACTGTACAACGCAGAGTCGACTTTTTTTTTGTTTGTTTCCTTTCTTT670.10393559095915486No Hit
GTCCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC660.10238431348215254No Hit
GTCCCGGGGAACTACAAGCCTCCAGCCCAGAAGAGCATCCAGGAGATCCAGGAACT660.10238431348215254No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGATTACTCTTCC650.10083303600515026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAAGA150.00800981350.59700437
TAAGAAC150.0080348450.55713334
ACATTGA150.0080348450.55713335
GCATAAG150.0080348450.55713336
TCGGACA150.00811027250.43789333
AGGAAAG150.00813553250.3982731
GGCTAGG204.0883812E-450.16184638
TAAGACC150.00828832750.16184239
TCGTGGG150.00828832750.16184239
AACACTG150.00828832750.16184238
CAACCAA204.1042012E-450.12265849
AACCAAA204.1042012E-450.12265850
ATTCTGG204.1042012E-450.12265846
GCATTCT204.1042012E-450.12265844
CACGTTT150.00831399950.12265450
AGGAACT150.00831399950.12265450
CCCACTT150.00831399950.12265450
GCCAGCG301.0150034E-650.12265449
TATCCAA150.00831399950.12265444
TTCTACA150.00831399950.12265445
Produced by FastQC (version 0.11.5)