FastQCFastQC Report
Fri 17 Jun 2016
SRR1526487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229452
Sequences flagged as poor quality0
Sequence length64
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.8720778201976883No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG17250.7517912243083521No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC13490.5879225284591113No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9800.4271045796070638No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA9160.3992120356327249No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9050.3944180046371354No Hit
TATGTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC8250.3595523246692119No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8070.35170754667642906No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.3429911266844482No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.24057319177867267No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG5040.21965378379791853No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.17650750483761307No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.17302093684082073No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.14861496086327422No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC3320.14469257186688284No Hit
TATGTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3170.13815525687289715No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.13510450987570385No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.1316179418789115No Hit
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2960.1290030158813172No Hit
TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2860.12464480588532678No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10328957690497358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACA150.004628617358.1695953
AATCGCC258.499099E-658.1695951
TACGATG201.9781881E-458.16958654
TTACGAT201.9781881E-458.16958653
CCACTTA150.004632634658.1568545
CTAATCG258.510147E-658.15684549
TAATCGC258.510147E-658.15684550
ACGCGAG150.004640677558.1313842
CGCATCT150.004644702758.11865241
ATTACAC201.9932367E-458.0805138
TTGTCGG150.0046608358.06780237
CCGCAAC150.0046608358.06780237
TAGTTGT150.00466890958.04241634
TTACCGC150.00469320957.96637728
AAACGTA150.004697268457.95372427
CGATACT202.0148924E-457.95372427
TGCGAAG150.004701330357.94107426
CCGATGT150.004721678357.8779220
GTAAATC202.0279754E-457.87791420
TAGGTCG150.00472983657.852695
Produced by FastQC (version 0.11.5)