FastQCFastQC Report
Fri 17 Jun 2016
SRR1526486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222334
Sequences flagged as poor quality0
Sequence length64
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.7137909631455378No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG15030.6760099669866058No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC10860.4884543074833359No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT10260.46146788165552727No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7500.33733032284760767No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA7100.3193393722957352No Hit
TATGTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC6950.3125927658387831No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.29415204152311386No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.28335747119199045No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.18800543326706665No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4140.18620633821187943No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.14167873559599523No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.1412289618321984No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.1196398211699515No Hit
TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2600.11694117858717064No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.11514208353198341No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.10884525083882807No Hit
TATGTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.10524706072845358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATG201.9563935E-458.29920636
TCGCTAG150.00459192558.28597634
CGATAAC201.9585909E-458.28597334
CGGGACG150.00459605458.27274733
GCGATAA201.9607901E-458.27274733
TAACGGC351.5823389E-858.2331137
CGCTTGG150.00460845758.23310539
TGCGTCG150.004612596758.21990238
ACGCTCC201.9873347E-458.11450652
GCGGATT258.546058E-658.11450652
TCGAACT303.6969868E-758.11450653
ATCGAAC303.6969868E-758.11450652
AGCGATA201.9895595E-458.1013632
ACCGTGA150.00465414758.08821549
ATTTCGA150.00465414758.08821549
ACGTTAG150.004658317758.07507741
TAGCGAT201.9940152E-458.07507731
ATTCCCG150.004658317758.07507744
CACGTTA150.004658317758.07507740
CGGGTAG150.00466666758.04882430
Produced by FastQC (version 0.11.5)