FastQCFastQC Report
Fri 17 Jun 2016
SRR1526485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198420
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT21311.0739844773712326No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA13080.6592077411551255No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC12750.6425763531902026No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9640.4858381211571414No Hit
TATGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.46618284447132347No Hit
TATGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.3936095151698417No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT7240.3648825723213386No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6570.3311158149380103No Hit
TATGTAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG6260.3154923898800524No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC5060.25501461546215104No Hit
TATGTAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG5010.2524947081947384No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.1960487854046971No Hit
TATGTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.1955448039512146No Hit
TATGTAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.1839532305211168No Hit
TATGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.170345731277089No Hit
TATGTAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG2970.149682491684306No Hit
TATGTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.14665860296341093No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2590.13053119645197056No Hit
TATGTAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.12549138191714546No Hit
TATGTAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.12498740046366294No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.12347545610321539No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC2440.12297147464973288No Hit
TATGTAGGGATCCATAGCTGTACATCCGATACTGCTAATTACACACATGTGATGTT2250.11339582703356517No Hit
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2230.11238786412660015No Hit
TATGTAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA2190.11037193831267009No Hit
TATGTAGGGATCACCTCTCAAGCCTCTACTTTGTTTCCGTGTGTGTGTGTGTGTGT2150.10835601249874005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT150.00801050750.6355537
ACTCACA150.00801050750.6355536
GCCGTTC150.00801050750.6355537
ACATGCG203.9279912E-450.62256235
CGAACAC150.00812484450.45438433
TGTGAGC150.008133057550.44149432
TGCGATC150.008133057550.44149432
GTGCGAT203.998095E-450.4414931
AACATCG252.033069E-550.19781549
GCTACTA204.0948222E-450.1978140
CGGCCCA150.00829862250.18505546
TTCCGTA150.00829862250.18505545
TACGGAC150.00829862250.18505539
CGTTCAC150.00829862250.18505539
ACGGCGG150.00829862250.18505546
TAACACG150.00829862250.18505539
GAAACTA150.00829862250.18505546
CACGGTT204.099964E-450.1850544
AACGATA204.099964E-450.1850544
TGCGACT150.00830696650.17230243
Produced by FastQC (version 0.11.5)