FastQCFastQC Report
Fri 17 Jun 2016
SRR1526484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32386
Sequences flagged as poor quality0
Sequence length64
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6271.9360217377879332No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCACTTTTTATA3331.0282220712653616No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.93867720620021No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.7410609522633237No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.5434446983264374No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1640.5063916507132712No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.3489161983573149No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.327301920582968No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1060.327301920582968No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG970.2995121348730933No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.2902488729698018No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG820.2531958253566356No Hit
GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.13894892854937319No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC440.13586117458160935No Hit
GGTACAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCCGAAAGA410.1265979126783178No Hit
GGTACAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.11733465077502625No Hit
GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.11115914283949854No Hit
GGTACAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCGAAAGA360.11115914283949854No Hit
GGTACAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.11115914283949854No Hit
GGTACAGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1080713888717347No Hit
GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1080713888717347No Hit
GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.10498363490397085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCAC303.6060737E-758.09612750
CCTACTA303.6060737E-758.09612737
CAGCAGA150.004615754458.09612742
TCCTACT303.6060737E-758.09612736
ACCATCC303.6060737E-758.09612746
CATCCTC303.6060737E-758.09612748
TGATCCT150.004615754458.09612751
TTTTATA303.6060737E-758.09612758
AGTACGT406.730261E-1058.09612738
AACCATC303.6060737E-758.09612745
TACGTAG406.730261E-1058.09612740
CCATCCT303.6060737E-758.09612747
CCTCACT303.6060737E-758.09612751
TTGATCC150.004615754458.09612750
CTAAAAC303.6060737E-758.09612741
CATGGCT258.39805E-658.09612755
CTCACTT303.6060737E-758.09612752
AGTTTGA406.730261E-1058.09612747
CTACTAA303.6060737E-758.09612738
GTACGTA406.730261E-1058.09612739
Produced by FastQC (version 0.11.5)