FastQCFastQC Report
Fri 17 Jun 2016
SRR1526483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31581
Sequences flagged as poor quality0
Sequence length64
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5591.7700516133118012No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCACTTTTTATA2880.9119407238529496No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.8201133592983122No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.6206263259554795No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1380.436971596846205No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.4084734492258003No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG960.30398024128431655No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.25015040689021883No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.24381748519679552No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG750.23748456350337227No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.20898641588296762No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG600.18998765080269783No Hit
GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.15515658148886988No Hit
GGTACAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.12982489471517683No Hit
GGTACAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCGAAAGA370.11715905132833032No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.11082612963490707No Hit
GGTACAGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.11082612963490707No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC330.10449320794148381No Hit
GGTACAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.10449320794148381No Hit
GGTACAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.10132674709477218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG150.00443202858.68932339
ACCGTAG150.00443202858.68932339
GCTACCG150.00443202858.68932336
TACCGTA150.00443202858.68932338
CTACCGT150.00443202858.68932337
GTTGCCG150.00443202858.68932337
TTGCCGT150.00443202858.68932338
CCTACTA550.058.6893237
TCCTACT550.058.6893236
CTACTAA550.058.6893238
TACTAAA550.058.6893239
TTCGCTA150.004460559758.5945133
TTTCCTA600.058.5945134
ATGCGTT150.004460559758.5945133
TTCCTAC600.058.5945135
TCGCTAC150.004460559758.5945134
GCGTTGC150.004460559758.5945135
TTTTATA501.8189894E-1258.405858
ATCCTGG201.9128494E-458.40579653
AAATTCG150.00451803358.40579648
Produced by FastQC (version 0.11.5)