FastQCFastQC Report
Fri 17 Jun 2016
SRR1526482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28203
Sequences flagged as poor quality0
Sequence length56
%GC35

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7092.513916959188739No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3381.198454065170372No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCAC2831.0034393504237138No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.9608906853880793No Hit
GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1590.5637698117221572No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.5318583129454314No Hit
GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1030.3652093748891962No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.3545722086302876No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.32620643193986454No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA830.29429493316313865No Hit
GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC810.28720348899053294No Hit
GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.21983476935077828No Hit
GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.2021061589192639No Hit
GGTACAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA550.19501471474665816No Hit
GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.170194660142538No Hit
GGTACAGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.14182888345211503No Hit
GGTACAGGGGGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCAGTCCACA390.13828316136581215No Hit
GGTACAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC390.13828316136581215No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA380.1347374392795093No Hit
GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1311917171932064No Hit
GGTACAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC350.12410027302060064No Hit
GGTACAGGGTCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.12410027302060064No Hit
GGTACAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.12410027302060064No Hit
GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.12055455093429776No Hit
GGTACAGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT320.11346310676169202No Hit
GGTACAGGGGGCATGGCAACGCAGAGTTCGACCTTTTTTTTTTTTTTTTTTTTTTT300.10637166258908627No Hit
GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.10637166258908627No Hit
GGTACAGGGATGCCGTGCTCCTCAAACCTCGAATTTCTGTAAGCTAGATGGAGAGA290.10282594050278339No Hit
GGTACAGGGAAGTGGTCAAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.10282594050278339No Hit
GGTACAGGGCAGTTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT290.10282594050278339No Hit
GGTACAGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC290.10282594050278339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACC203.8422097E-450.6949535
GCTACCG203.8422097E-450.6949536
CGGGCAA203.8422097E-450.6949534
TCGCTAC203.8422097E-450.6949534
CCTACTA700.050.69494637
TTTCCTA700.050.69494634
TTCCTAC700.050.69494635
GCAAACT251.8751382E-550.69494637
TGTTCGG150.00790185150.69494235
GTTCGGC150.00790185150.69494236
TTCGCTA203.9113947E-450.51259233
TTCCGGG203.9113947E-450.51259231
GGTTCGC203.9113947E-450.51259231
GAATGTT150.00801552350.51259232
TCCGGGC203.9113947E-450.51259232
CCGGGCA203.9113947E-450.51259233
AATGTTC150.00801552350.51259233
GTTCGCT203.9113947E-450.51259232
AACTGGA204.052734E-450.15178740
CTACTAA700.050.15178738