##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526482_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28203 Sequences flagged as poor quality 0 Sequence length 56 %GC 35 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.69060029074921 38.0 38.0 40.0 34.0 40.0 2 37.33063858454774 38.0 38.0 40.0 33.0 40.0 3 34.890579016416694 36.0 33.0 39.0 28.0 40.0 4 37.143601744495264 38.0 38.0 40.0 33.0 40.0 5 37.08931673935397 38.0 38.0 40.0 33.0 40.0 6 37.477679679466725 38.0 38.0 40.0 33.0 40.0 7 37.696982590504554 38.0 38.0 40.0 35.0 40.0 8 37.968762188419674 38.0 38.0 40.0 35.0 40.0 9 37.89192639080949 38.0 38.0 40.0 35.0 40.0 10 36.632308619650395 38.0 36.0 40.0 31.0 40.0 11 37.87185760380101 39.0 38.0 40.0 35.0 40.0 12 37.78246995000532 39.0 38.0 40.0 34.0 40.0 13 37.71003084778215 39.0 38.0 40.0 34.0 40.0 14 37.70464135021097 39.0 38.0 40.0 34.0 40.0 15 37.718044179697195 39.0 38.0 40.0 33.0 40.0 16 37.641314753749604 39.0 38.0 40.0 34.0 40.0 17 37.707761585646914 39.0 38.0 40.0 34.0 40.0 18 37.60330461298443 39.0 38.0 40.0 33.0 40.0 19 37.378328546608515 39.0 37.0 40.0 33.0 40.0 20 37.53192922738716 39.0 38.0 40.0 33.0 40.0 21 37.33553168102684 39.0 37.0 40.0 33.0 40.0 22 37.15707548842322 39.0 36.0 40.0 33.0 40.0 23 36.98042761408361 39.0 36.0 40.0 33.0 40.0 24 36.86597170513775 39.0 36.0 40.0 32.0 40.0 25 36.92600078005886 39.0 36.0 40.0 32.0 40.0 26 36.64954082898982 39.0 36.0 40.0 32.0 40.0 27 36.68804737084707 39.0 36.0 40.0 32.0 40.0 28 36.47962982661419 39.0 36.0 40.0 31.0 40.0 29 36.525972414282165 39.0 36.0 40.0 31.0 40.0 30 36.46066021345247 39.0 36.0 40.0 31.0 40.0 31 36.37045704357693 39.0 36.0 40.0 31.0 40.0 32 35.99719887955182 39.0 35.0 40.0 30.0 40.0 33 36.32663191859022 39.0 36.0 40.0 31.0 40.0 34 36.38460447470127 39.0 36.0 40.0 31.0 40.0 35 36.065028543062795 39.0 35.0 40.0 30.0 40.0 36 35.92022125305819 39.0 35.0 40.0 30.0 40.0 37 35.50214516186222 39.0 35.0 40.0 29.0 40.0 38 35.66517746339042 39.0 35.0 40.0 29.0 40.0 39 35.64354855866397 39.0 35.0 40.0 29.0 40.0 40 35.295996879764566 39.0 35.0 40.0 28.0 40.0 41 35.25667482182747 39.0 35.0 40.0 28.0 40.0 42 35.14282168563628 39.0 35.0 40.0 27.0 40.0 43 35.01322554338191 39.0 35.0 40.0 26.0 40.0 44 34.46108570010283 39.0 35.0 40.0 23.0 40.0 45 34.55320355990497 39.0 35.0 40.0 24.0 40.0 46 34.04453426940396 39.0 35.0 40.0 18.0 40.0 47 33.96443640747438 39.0 35.0 40.0 17.0 40.0 48 33.5675637343545 39.0 34.0 40.0 10.0 40.0 49 33.42825231358366 39.0 34.0 40.0 9.0 40.0 50 33.2319965961068 39.0 34.0 40.0 4.0 40.0 51 32.59280927560898 39.0 33.0 40.0 4.0 40.0 52 32.51239229869163 39.0 34.0 39.0 4.0 40.0 53 32.06244016593979 38.0 33.0 39.0 4.0 40.0 54 31.608197709463532 38.0 32.0 39.0 4.0 40.0 55 30.831826401446655 37.0 31.0 39.0 4.0 40.0 56 18.2231677481119 21.0 15.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.0805889633772878 1 2 1.915269348698466 1 3 2.9352284801203723 1 4 1.955327851298513 1 5 2.170135114319102 1 6 1.4212601953516781 1 7 1.1229861763422164 1 8 0.870651091950208 1 9 0.7114041078350013 1 10 1.9621034453036046 1 11 0.3654637992386327 1 12 0.6554392494564212 1 13 0.7465777655218702 1 14 1.0960617187145942 1 15 0.8116092729761135 1 16 1.0332829955812315 1 17 1.2080242395285055 1 18 1.3521920164346284 1 19 1.0012319731834864 1 20 1.2267448780638475 1 21 1.3832526823858942 1 22 1.7457239885090061 1 23 0.6810254586001747 1 24 1.688677260751028 1 25 1.9267742511457442 1 26 1.7828212993828032 1 27 1.658431657696461 1 28 2.029284351848851 1 29 2.1911509749056464 1 30 0.27982673481270837 1 31 1.0064925665751545 1 32 -0.24999817890317644 1 33 -0.039420458105063005 1 34 -0.15437312334248077 1 35 0.25441392682327546 1 36 -0.4172099266557723 1 37 0.3618472631591487 1 38 0.6470080995412175 1 39 -0.24908447165647374 1 40 0.4844221419152248 1 41 0.5259214877545233 1 42 0.02709150554397155 1 43 0.7398562872542627 1 44 1.284018646091674 1 45 0.8598004198489448 1 46 1.1783843238588076 1 47 1.778348838771933 1 48 0.9680747769543103 1 49 2.2110651063171076 1 50 1.063061880345714 1 51 -1.0486003340277605 1 52 -1.0113841809746305 1 53 -0.7963897493914125 1 54 -0.3397606108359348 1 55 0.28142845725729515 1 56 0.6100373643744419 2 1 1.0088648254462527 2 2 1.6835452107674342 2 3 2.06350434218934 2 4 1.3622244030226511 2 5 1.4446178729397943 2 6 1.579880885006851 2 7 1.2871241073766981 2 8 0.920306264364001 2 9 1.1445075561108666 2 10 1.8545172384070483 2 11 0.6137396613075978 2 12 0.6113013184219369 2 13 1.1107156965563547 2 14 0.6574410290594201 2 15 0.22816099711404547 2 16 0.6056967886846749 2 17 1.2066449291836818 2 18 0.897019602641528 2 19 1.1805423180110708 2 20 0.6557103953052277 2 21 0.990149234110028 2 22 0.00917226437107388 2 23 0.7872323551519003 2 24 0.45005657109585684 2 25 1.0867742511457408 2 26 0.887648885589698 2 27 0.5963626921792198 2 28 1.2499740070212653 2 29 0.7001164921470249 2 30 1.2770681141230469 2 31 1.0189063596786028 2 32 1.322415614200267 2 33 1.3978209212052803 2 34 1.2594199801057968 2 35 1.8116553061336163 2 36 1.9234797285166394 2 37 2.0721920907453537 2 38 1.5201115478170806 2 39 2.0391913904124976 2 40 2.2237324867428114 2 41 2.4307490739614224 2 42 2.595367367612937 2 43 2.2653735286335746 2 44 3.3322945081606434 2 45 1.9342831784696344 2 46 1.7176946686863914 2 47 1.9755902180822744 2 48 2.267385121781899 2 49 2.154513382179182 2 50 2.827199811380197 2 51 3.1776065625239625 2 52 2.841029612128814 2 53 2.6463688712982467 2 54 2.729204906405446 2 55 3.5517732848435024 2 56 2.7810718471330595 3 1 -0.11588162485801234 3 2 -0.9706130042427077 3 3 -0.06124210811492503 3 4 -0.37408391340736813 3 5 0.6160174672602778 3 6 0.0565543129987347 3 7 0.2688685292833881 3 8 -0.034054790402734625 3 9 -1.354478245106172 3 10 0.03504462177419043 3 11 0.2242873286503979 3 12 -0.4292666328965211 3 13 -0.8487163521251873 3 14 -0.9957029871677605 3 15 -0.6095671976121153 3 16 -0.9490699455952409 3 17 0.03625953364615242 3 18 -0.6536903365065427 3 19 -1.1987680268165164 3 20 -1.5897257101714501 3 21 -1.3932179058494043 3 22 -1.1530995409027653 3 23 -0.6519157178704091 3 24 -1.5560286216019108 3 25 -1.2661669253248462 3 26 -0.8689434064995538 3 27 -0.7768624599505998 3 28 -1.6648332952099736 3 29 -0.8394372603884719 3 30 -1.7989967945990628 3 31 -0.6535074334248421 3 32 -1.0852922965502358 3 33 -1.4723616345756483 3 34 -1.9779025351071837 3 35 -3.8232331320002544 3 36 -2.4030922795969474 3 37 -1.9734468544879107 3 38 -1.4200507239881972 3 39 -2.2326138834211733 3 40 -1.9791072698494787 3 41 -2.5611373357748874 3 42 -2.235261435632502 3 43 -1.553084889216322 3 44 -0.8512754715553825 3 45 -0.864905462503998 3 46 -1.8674980290823733 3 47 0.07599589759546177 3 48 -0.8178075759868619 3 49 -0.801876070153476 3 50 -0.8381145902425189 3 51 -3.6144826869689375 3 52 -3.0960900633275728 3 53 -1.6305073964502341 3 54 -2.7515253167182863 3 55 -1.758571542742704 3 56 -1.0029038120961467 4 1 0.3105889633772847 4 2 0.9138407772698969 4 3 1.222371337263226 4 4 0.6553278512985159 4 5 0.9101351143191039 4 6 1.035545909637392 4 7 1.1344147477707835 4 8 0.6235082348073533 4 9 0.9228326792635713 4 10 1.2178177310178881 4 11 0.12974951352434516 4 12 0.5854392494564209 4 13 0.6722920512361554 4 14 0.4874902901431639 4 15 0.43875213011897074 4 16 0.11185442415266067 4 17 -0.48054718904292315 4 18 0.3021920164346312 4 19 0.372660544612053 4 20 -0.0203979790790072 4 21 0.6761098252430386 4 22 0.7271525599375721 4 23 0.23673974431446254 4 24 0.8158201178938853 4 25 -0.02036860599711332 4 26 -0.4928929863314835 4 27 -0.4344254851606877 4 28 -0.19214421957971695 4 29 -0.11884902509435591 4 30 -0.9187446937587254 4 31 -0.5820788619962727 4 32 0.11428753538253744 4 33 -0.1908490295336307 4 34 0.13134116237180393 4 35 0.365842498251844 4 36 1.6956472162013725 4 37 1.297561548873432 4 38 1.7984366709697852 4 39 1.7337726712006756 4 40 2.130136427629509 4 41 1.5502072020402409 4 42 1.4685200769725384 4 43 1.4784277158256955 4 44 2.0983043603773908 4 45 2.399800419848944 4 46 2.8026700381445195 4 47 3.029777410200502 4 48 3.638074776954312 4 49 2.908207963459965 4 50 3.460204737488567 4 51 1.0556853802579553 4 52 1.454330104739654 4 53 -0.33638974939141164 4 54 0.30309653202120757 4 55 0.7057141715430113 4 56 0.38575165008872503 5 1 0.3192096530324591 5 2 0.5456141762846727 5 3 1.8221250318445072 5 4 0.3967071616433415 5 5 1.341169597077723 5 6 0.7178119194896055 5 7 0.9767792797904917 5 8 0.5754786781571042 5 9 0.4893351423177634 5 10 0.5786551694415323 5 11 0.19994655785932025 5 12 -0.04387109537116629 5 13 -0.37204292413330364 5 14 0.07123413250769772 5 15 0.021264445389910236 5 16 -0.08395838372911157 5 17 -0.5864585190921829 5 18 0.034950637124289585 5 19 -0.88842319923031 5 20 -0.8615309840051211 5 21 -0.5615749038210041 5 22 -1.1977242873530685 5 23 -0.7644917827791318 5 24 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3.7953695726534065 6 51 4.3221688967414735 6 52 2.7455388959484495 6 53 2.509764096762435 6 54 3.044854773779452 6 55 1.202966918795756 6 56 -0.8807318663947896 7 1 0.4534461062344306 7 2 0.9495550629841816 7 3 0.2580856229775179 7 4 0.6196135655842312 7 5 0.9458494000333886 7 6 0.785545909637392 7 7 1.0272718906279295 7 8 0.7306510919502074 7 9 0.8514041078350019 7 10 1.360674873875034 7 11 0.772606656381484 7 12 0.2282963923135668 7 13 0.6722920512361554 7 14 0.7017760044288792 7 15 0.7601807015475472 7 16 0.7189972812955148 7 17 0.9123099538142228 7 18 0.12362058786320063 7 19 1.265517687469199 7 20 0.9081734494924163 7 21 0.8903955395287468 7 22 1.0842954170804333 7 23 0.6295968871716084 7 24 0.7443915464653159 7 25 1.1939171082885949 7 26 -0.4928929863314835 7 27 0.3512888005536041 7 28 -0.08500136243686285 7 29 -0.11884902509435591 7 30 0.6883981633841358 7 31 0.7750639951465885 7 32 1.6500018210968221 7 33 1.8091509704663693 7 34 2.02419830522895 7 35 1.5444139268232746 7 36 2.731361501915657 7 37 2.904704406016293 7 38 2.5127223852555005 7 39 2.6980583854863838 7 40 2.5587078562009395 7 41 2.657350059183095 7 42 2.754234362686823 7 43 2.3355705729685496 7 44 1.812590074663106 7 45 2.899800419848944 7 46 1.124098609573089 7 47 1.0654916959147869 7 48 0.24521763409716613 7 49 0.44392224917425693 7 50 -0.5755095482257175 7 51 -1.8014574768849023 7 52 -1.5813841809746307 7 53 -2.4078183208199846 7 54 -2.30404632512165 7 55 -1.6157143998855616 7 56 -1.4713912070541326 8 1 0.4605889633772833 8 2 1.1019360153651334 8 3 1.2818951467870363 8 4 0.521994517965183 8 5 0.8768017809857724 8 6 0.7545935286850067 8 7 1.0963195096755456 8 8 0.8639844252835402 8 9 0.9847374411683347 8 10 0.9154367786369377 8 11 -0.20120286742803728 8 12 -0.2645607505435805 8 13 -0.04675556781145929 8 14 0.8160617187145931 8 15 0.16494260630945234 8 16 0.03328299558123149 8 17 0.21469090619517317 8 18 0.31885868310129695 8 19 0.034565306516817884 8 20 0.6200782113971783 8 21 0.01658601571922702 8 22 0.072390655175667 8 23 1.1343587919335079 8 24 0.5420105940843669 8 25 -0.5132257488542606 8 26 -0.35717870061719736 8 27 0.2917649910297939 8 28 -0.037382314817811846 8 29 0.2978176415723084 8 30 0.19316006814603526 8 31 -0.5201741000915092 8 32 0.283335154430155 8 33 -0.15275379143839274 8 34 0.07896020999085351 8 35 0.8610805934899446 8 36 -0.4972099266557706 8 37 0.4951805964924816 8 38 -0.906325233792117 8 39 -0.675751138323136 8 40 -0.4555778580847729 8 41 -0.08074517891213873 8 42 -0.9529084944560324 8 43 -1.400143712745738 8 44 0.21735197942501117 8 45 -0.03353291348438603 8 46 0.8717176571921357 8 47 0.6250155054385971 8 48 1.5880747769543149 8 49 1.6510651063171125 8 50 1.3697285470123788 8 51 2.52473299930557 8 52 1.935282485692035 8 53 1.6636102506085884 8 54 1.7269060558307352 8 55 1.1414284572572946 8 56 1.5167040310411082 9 1 -0.6179824651941459 9 2 0.19955506298418157 9 3 0.615228480120372 9 4 0.26247070844137 9 5 0.267277971461958 9 6 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>>Per sequence quality scores pass #Quality Count 4 15.0 5 4.0 6 2.0 7 8.0 8 13.0 9 13.0 10 17.0 11 23.0 12 24.0 13 31.0 14 39.0 15 55.0 16 64.0 17 68.0 18 87.0 19 107.0 20 112.0 21 145.0 22 146.0 23 160.0 24 236.0 25 254.0 26 242.0 27 270.0 28 340.0 29 433.0 30 559.0 31 806.0 32 893.0 33 1161.0 34 1578.0 35 1957.0 36 2614.0 37 4563.0 38 5867.0 39 5297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 98.91817117724257 0.8158053417514987 0.170255027843791 0.09576845316213244 8 97.79101514023331 0.7304187497783924 0.20919760309186966 1.2693685068964293 9 95.62103322341595 2.1238875296954225 1.567209162145871 0.6878700847427578 10 22.84154167996312 47.99843988228203 10.154948055171435 19.005070382583416 11 23.469134489238733 39.78654753040456 18.437754848774954 18.306563131581747 12 46.356770556323795 19.49438003049321 19.86313512746871 14.285714285714285 13 24.493848172180265 18.356203240789988 30.450661277169093 26.699287309860654 14 27.64954082898982 30.418749778392367 21.299152572421374 20.63255682019643 15 34.84735666418466 22.90891039960288 25.518561855121796 16.725171081090664 16 20.288621777825053 27.514803389710313 26.19934049569195 25.997234336772685 17 25.160443924405207 28.355139524164098 18.537035067191436 27.947381484239266 18 26.926213523384035 28.390596745027125 22.806084459100095 21.87710527248874 19 18.792327057405238 30.525121440981458 26.94394213381555 23.738609367797753 20 22.674892741906888 29.879800021274335 19.685849023153565 27.759458213665212 21 23.03301067262348 24.72077438570365 26.543275538063327 25.70293940360954 22 23.94426124880332 26.16388327482892 24.02935857887459 25.862496897493177 23 28.493422685529907 30.010991738467542 24.188916072758218 17.306669503244336 24 20.35599049746481 33.87937453462398 26.568095592667447 19.196539375243766 25 22.463459628210586 22.761458776784448 31.733361714204626 23.04171988080034 26 22.200532386867792 21.817213842058564 34.78970718722271 21.192546583850934 27 17.386363636363637 20.454545454545457 37.28338068181819 24.875710227272727 28 14.850992789400774 26.12510212055554 41.686498774553336 17.337406315490355 29 19.01293348493462 19.16216600341103 44.052018192154634 17.772882319499715 30 14.160204880130895 22.995660525005334 48.506082378885964 14.338052215977804 31 14.856837986839766 18.69820380579762 54.095678463453666 12.349279743908946 32 8.444539340237002 16.422903099533826 63.75929682217715 11.373260738052027 33 9.662489319282255 17.01438336656223 59.794218171461125 13.52890914269439 34 9.45989247694663 19.389753266635807 62.142628262185355 9.007725994232207 35 7.6745014245014245 21.655982905982906 61.90883190883191 8.76068376068376 36 7.944707684634294 17.560297837471943 63.53628558195874 10.958708895935017 37 7.1541615711099915 15.862168189338895 65.04568016689447 11.937990072656643 38 7.5014261266400455 17.12778094694809 66.7997718197376 8.571021106674273 39 7.094474153297682 17.693404634581107 66.3921568627451 8.819964349376114 40 7.554380826517127 18.223381076543916 66.342822445262 7.879415651676966 41 7.571000428143285 18.856143856143856 64.75667189952904 8.816183816183816 42 8.070100296248706 16.154477638576576 66.49891137523647 9.27651068993825 43 9.55798343330477 17.78063410454156 65.31348186232505 7.347900599828621 44 7.663451232583066 18.674526616648805 66.0128617363344 7.649160414433727 45 8.671263710743506 20.768873486012364 63.77148165350673 6.788381149737396 46 7.806412410194088 19.87704185581013 63.452121385423744 8.864424348572042 47 9.13963127054452 19.165356581392025 63.802343861655 7.892668286408461 48 10.17822632596092 19.722997637964355 61.75649559802447 8.342280438050247 49 7.148990983254616 23.22169743809933 62.963360526692426 6.665951051953628 50 8.747137950772753 20.510160274756725 63.61977676016027 7.122925014310247 51 7.350837029617971 21.637573329517814 63.15281156102447 7.858778079839748 52 8.525411596277738 21.01646385110952 60.34359341445955 10.114531138153184 53 8.885229469463736 22.614018758502183 60.44605140688767 8.054700365146417 54 10.18903050264929 22.433051696978374 59.00400973793498 8.373908062437348 55 8.958751074190777 25.3258378688055 57.175594385562874 8.539816671440848 56 9.419978517722878 24.042248478338703 56.308628714643746 10.229144289294666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 3.0 7 3.0 8 5.5 9 8.0 10 34.0 11 60.0 12 53.5 13 65.0 14 83.0 15 116.0 16 149.0 17 383.0 18 617.0 19 729.5 20 842.0 21 898.5 22 1015.0 23 1075.0 24 1360.5 25 1646.0 26 2010.5 27 2375.0 28 2642.5 29 2906.0 30 2902.0 31 2741.5 32 2581.0 33 2022.5 34 1464.0 35 1519.0 36 1574.0 37 1250.5 38 664.0 39 401.0 40 370.5 41 340.0 42 370.0 43 400.0 44 378.0 45 356.0 46 376.5 47 389.5 48 382.0 49 395.5 50 409.0 51 464.0 52 519.0 53 742.0 54 885.0 55 805.0 56 723.5 57 642.0 58 600.0 59 558.0 60 522.0 61 486.0 62 426.0 63 324.5 64 283.0 65 246.5 66 210.0 67 188.5 68 167.0 69 147.5 70 128.0 71 104.5 72 62.0 73 43.0 74 32.5 75 22.0 76 17.5 77 13.0 78 9.0 79 3.5 80 2.0 81 2.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.03545722086302876 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.053185831294543136 26 0.09928021841648052 27 0.15246604971102365 28 0.1772861043151438 29 0.20919760309186966 30 0.31556926568095595 31 0.31202354359465306 32 0.3616636528028933 33 0.40775803992483073 34 0.41130376201113356 35 0.4361238166152537 36 0.4751267595645853 37 1.421834556607453 38 0.5495869233769457 39 0.5424954792043399 40 0.7304187497783924 41 0.6205013651030032 42 0.6595043080523348 43 0.6914158068290608 44 0.7552388043825126 45 0.7587845264688153 46 0.8013331915044499 47 0.7623302485551183 48 0.9254334645250505 49 0.9041591320072333 50 0.8899762436620218 51 0.8757933553168102 52 0.9325249086976563 53 0.9537992412154737 54 0.9608906853880793 55 0.9750735737332908 56 0.967982129560685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 28203.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.724993794986354 #Duplication Level Percentage of deduplicated Percentage of total 1 73.75239923224568 38.14842392653264 2 12.167534960241294 12.587313406375209 3 5.237181244858788 8.12679502180619 4 2.762544557170277 5.715704003120235 5 1.7343021661639706 4.4853384391731375 6 1.055662188099808 3.276247207743857 7 0.7746092678914176 2.8046661702655746 8 0.527831094049904 2.1841648051625713 9 0.39758705785577186 1.850866929050101 >10 1.4943789415958322 11.803708825302273 >50 0.04798464491362764 1.879232705740524 >100 0.04112969564025226 4.62362160053895 >500 0.006854949273375377 2.513916959188739 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 709 2.513916959188739 No Hit GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 338 1.198454065170372 No Hit GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCCTCAC 283 1.0034393504237138 No Hit GGTACAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.9608906853880793 No Hit GGTACAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 159 0.5637698117221572 No Hit GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.5318583129454314 No Hit GGTACAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 103 0.3652093748891962 No Hit GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.3545722086302876 No Hit GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.32620643193986454 No Hit GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 83 0.29429493316313865 No Hit GGTACAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 81 0.28720348899053294 No Hit GGTACAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.21983476935077828 No Hit GGTACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.2021061589192639 No Hit GGTACAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.19501471474665816 No Hit GGTACAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.170194660142538 No Hit GGTACAGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.14182888345211503 No Hit GGTACAGGGGGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCAGTCCACA 39 0.13828316136581215 No Hit GGTACAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC 39 0.13828316136581215 No Hit GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 38 0.1347374392795093 No Hit GGTACAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.1311917171932064 No Hit GGTACAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 35 0.12410027302060064 No Hit GGTACAGGGTCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.12410027302060064 No Hit GGTACAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.12410027302060064 No Hit GGTACAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.12055455093429776 No Hit GGTACAGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 32 0.11346310676169202 No Hit GGTACAGGGGGCATGGCAACGCAGAGTTCGACCTTTTTTTTTTTTTTTTTTTTTTT 30 0.10637166258908627 No Hit GGTACAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.10637166258908627 No Hit GGTACAGGGATGCCGTGCTCCTCAAACCTCGAATTTCTGTAAGCTAGATGGAGAGA 29 0.10282594050278339 No Hit GGTACAGGGAAGTGGTCAAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.10282594050278339 No Hit GGTACAGGGCAGTTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.10282594050278339 No Hit GGTACAGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC 29 0.10282594050278339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0070914441726057514 0.0 0.0 0.0 0.0 7 0.0070914441726057514 0.0 0.0 0.0 0.0 8 0.0070914441726057514 0.0 0.0 0.0 0.0 9 0.014182888345211503 0.0 0.0 0.0 0.0 10 0.02482005460412013 0.0 0.0 0.0 0.0 11 0.04254866503563451 0.0 0.0 0.0 0.0 12 0.046094387121937384 0.0 0.0 0.0 0.0 13 0.046094387121937384 0.0 0.0 0.0 0.0 14 0.06027727546714889 0.0 0.0 0.0 0.0 15 0.06382299755345176 0.0 0.0 0.0 0.0 16 0.06736871963975463 0.0 0.0 0.0 0.0 17 0.06736871963975463 0.0 0.0 0.0 0.0 18 0.07091444172605751 0.0 0.0 0.0 0.0 19 0.07800588589866327 0.0 0.0 0.0 0.0 20 0.08864305215757189 0.0 0.0 0.0 0.0 21 0.10282594050278339 0.0 0.0 0.0 0.0 22 0.12055455093429777 0.0 0.0 0.0 0.0 23 0.13473743927950926 0.0 0.0 0.0 0.0 24 0.13828316136581215 0.0 0.0 0.0 0.0 25 0.13828316136581215 0.0 0.0 0.0 0.0 26 0.14892032762472077 0.0 0.0 0.0 0.0 27 0.15955749388362941 0.0 0.0 0.0 0.0 28 0.18083182640144665 0.0 0.0 0.0 0.0 29 0.19146899266035527 0.0 0.0 0.0 0.0 30 0.20919760309186966 0.0 0.0 0.0 0.0 31 0.20919760309186966 0.0 0.0 0.0 0.0 32 0.21274332517817254 0.0 0.0 0.0 0.0 33 0.21274332517817254 0.0 0.0 0.0 0.0 34 0.22338049143708116 0.0 0.0 0.0 0.0 35 0.2623834343864128 0.0 0.0 0.0 0.0 36 0.2730206006453214 0.0 0.0 0.0 0.0 37 0.2801120448179272 0.0 0.0 0.0 0.0 38 0.2801120448179272 0.0 0.0 0.0 0.0 39 0.2801120448179272 0.0 0.0 0.0 0.0 40 0.28720348899053294 0.0 0.0 0.0 0.0 41 0.29429493316313865 0.0 0.0 0.0 0.0 42 0.31202354359465306 0.0 0.0 0.0 0.0 43 0.31911498776725883 0.0 0.0 0.0 0.0 44 0.32620643193986454 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTACC 20 3.8422097E-4 50.69495 35 GCTACCG 20 3.8422097E-4 50.69495 36 CGGGCAA 20 3.8422097E-4 50.69495 34 TCGCTAC 20 3.8422097E-4 50.69495 34 CCTACTA 70 0.0 50.694946 37 TTTCCTA 70 0.0 50.694946 34 TTCCTAC 70 0.0 50.694946 35 GCAAACT 25 1.8751382E-5 50.694946 37 TGTTCGG 15 0.007901851 50.694942 35 GTTCGGC 15 0.007901851 50.694942 36 TTCGCTA 20 3.9113947E-4 50.512592 33 TTCCGGG 20 3.9113947E-4 50.512592 31 GGTTCGC 20 3.9113947E-4 50.512592 31 GAATGTT 15 0.008015523 50.512592 32 TCCGGGC 20 3.9113947E-4 50.512592 32 CCGGGCA 20 3.9113947E-4 50.512592 33 AATGTTC 15 0.008015523 50.512592 33 GTTCGCT 20 3.9113947E-4 50.512592 32 AACTGGA 20 4.052734E-4 50.151787 40 CTACTAA 70 0.0 50.151787 38 >>END_MODULE