FastQCFastQC Report
Fri 17 Jun 2016
SRR1526481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23996
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.42923820636772797No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.4250708451408568No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAGTTCCTGGG850.3542257042840473No Hit
CATCCCGGGATGTCCGAGCTGGAGAAGGCCATGGTTGCCCTCATTGATGTCTTCCACCAGTACT690.28754792465410905No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA690.28754792465410905No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG670.2792132022003667No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA570.23753958993165528No Hit
CATCCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA570.23753958993165528No Hit
CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTTGGTGTGGC550.22920486747791297No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC530.22087014502417068No Hit
CATCCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.20420070011668612No Hit
CATCCCGGGGGATTCTCTCTGCGTTTGCTGTCGCTGCAGCAGCGGACGACCTCCTTAGGGCCAG490.20420070011668612No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGACCTGGACAA450.18753125520920155No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT450.18753125520920155No Hit
CATCCCCCTCCAGATCTGCTTGTCCTCTGGTCTCAGGTCAAGGGCCCTTTGTGGCCACAGGAGC410.17086181030171696No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC360.15002500416736123No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC340.14169028171361892No Hit
CATCCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA320.13335555925987663No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC310.1291881980330055No Hit
CATCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.1291881980330055No Hit
CATCCCGGGAGGGCTTGTTTTCTTAGACTAATAGGGGCAGTAAAGTAGACGGTGGCAGGATATC300.12502083680613435No Hit
CATCCCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC270.11251875312552093No Hit
CATCCCGGGATGAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.11251875312552093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCC150.004487922458.46088858
CATCAAT201.8946025E-458.46088453
ATAATTA201.8946025E-458.46088458
CAATAAT201.8946025E-458.46088456
ATCAATA201.8946025E-458.46088454
TTATCTC201.9145703E-458.3375547
ATTATCT201.9145703E-458.3375546
TATCTCA201.9145703E-458.3375548
AATTATC201.9347052E-458.21473745
ATCACAA201.9347052E-458.21473740
TTTATCA201.9347052E-458.21473737
ATTTATC201.9347052E-458.21473736
TGATTTA201.9754803E-457.97064634
AAGATCC150.004679313457.84937321
GATCCCC150.004679313457.84937323
AGATCCC150.004679313457.84937322
ACATGAT201.9961224E-457.8493731
GATACAT201.9961224E-457.8493722
TACATCA201.9961224E-457.8493724
ATACATC201.9961224E-457.8493723
Produced by FastQC (version 0.11.5)