FastQCFastQC Report
Fri 17 Jun 2016
SRR1526480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23179
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.33219724750852064No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.30199749773501877No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG560.241597998188015No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT550.23728374822037188No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAGTTCCTGGG510.2200267483497994No Hit
CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTTGGTGTGGC480.20708399844687003No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA460.19845549851158378No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA460.19845549851158378No Hit
CATCCCGGGATGTCCGAGCTGGAGAAGGCCATGGTTGCCCTCATTGATGTCTTCCACCAGTACT440.1898269985762975No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC430.18551274860865438No Hit
CATCCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA410.17688424867336813No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGACCTGGACAA400.172569998705725No Hit
CATCCCGGGGGATTCTCTCTGCGTTTGCTGTCGCTGCAGCAGCGGACGACCTCCTTAGGGCCAG390.16825574873808188No Hit
CATCCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.14668449889986626No Hit
CATCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.1337417489969369No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC290.12511324906165064No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC290.12511324906165064No Hit
CATCCCGGGAGGGCTTGTTTTCTTAGACTAATAGGGGCAGTAAAGTAGACGGTGGCAGGATATC270.11648474912636438No Hit
CATCCCGGGATGAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11217049915872125No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC250.10785624919107814No Hit
CATCCCCCTCCAGATCTGCTTGTCCTCTGGTCTCAGGTCAAGGGCCCTTTGTGGCCACAGGAGC250.10785624919107814No Hit
CATCCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA240.10354199922343502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTGC150.00447245458.5054735
TAAGCAA150.004511454658.3777331
AAGCAAC150.004511454658.3777332
TGCCAAT150.00455070858.25054639
GCTGTAG150.00462997757.9978351
GGCCACA150.00462997757.9978353
TGAGGAG150.00462997757.9978311
AATGAAT150.00462997757.9978317
CTCCCAA258.515384E-657.87229522
ACTCCCA258.515384E-657.87229521
AATCCTT150.00466999557.8722921
GGATGAT258.625802E-657.7473038
GGATGAA258.625802E-657.7473038
CGGGGCT258.625802E-657.7473036
CCCGGGG6100.057.7473034
CGGGGAT258.625802E-657.7473036
CATCCCA258.625802E-657.7473031
GGGATCA258.625802E-657.7473037
ATGAGGA258.625802E-657.74730310
ATCCCGC150.0047102757.74732
Produced by FastQC (version 0.11.5)